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pcold-6xHis-HRV3Csite-HsPACT

Plasmid

#41097

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

PACT (PRKRA Human)

Tags

6xHis-HRV3Csite

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
pminiTol2

Plasmid

#31829

Depositor

Stephen Ekker

Article

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Type

Empty backbone

Use

Cloning vector

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pME-2PH-PLCd

Plasmid

#71283

Purpose

Multisite gateway entry clone for adding C-terminal fusions of human 2PH-PLC-delta

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

2PH-PLC delta (Human)

Use

Gateway multisite middle entry clone

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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FUW-M2-PPP1R12C S452A

Plasmid

#31679

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

Protein Phosphatase 1 Regulatory Subunit 12C S452A (PPP1R12C Human)

Use

Lentiviral

Tags

M2

Expression

Mammalian

Mutation

changed Serine 452 to Alanine

Available Since

April 12, 2012

Availability

Academic Institutions and Nonprofits only
Nluc CD9

Plasmid

#247322

Purpose

This plasmid can be used to quantify CD9 by luminescence signal upon providing substrate, in particular in the secretome, especially in the extracellular vesicles of cells expressing this construct.

Depositor

Clotilde Thery

Article

Grisard et al J Extracell Vesicles. 2022 Jul;11(7):e12

Insert

CD9 (CD9 Human)

Use

Luciferase

Tags

Nanoluciferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pSHARK2

Plasmid

#248168

Purpose

pBBR1 plasmid with constituitive lambda-CI (J23101-Bujard-Lambda-CI) and PirWT (PTet-B0033-PirWT) expression

Depositor

Thomas Gorochowski

Article

Hina-Nilesh Joshi et al ACS Synth Biol. 2026 Jan 13. doi: 10.102

Insert

LambdaCI-PirWT

Expression

Bacterial

Mutation

None

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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NanoLuc-ERN1 Fusion Vector

Plasmid

#238856

Purpose

Express NanoLuc-ERN1 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

ERN1 (ERN1 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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IKKε v2-V5

Plasmid

#169770

Purpose

Express IKKε v2 in mammalian cells

Depositor

Sung-Liang Yu

Article

Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1

Insert

Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 2

Tags

V5 tag

Expression

Bacterial and Mammalian

Available Since

July 30, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-Grik1-GFP

Plasmid

#233645

Purpose

Expresses GFP in OFF cone bipolar cells

Depositor

Connie Cepko

Article

Avilés et al J Gen Physiol. 2025 Mar 3;157(2):e202413

Insert

Grik1 promoter (Grik1 Mouse)

Use

AAV

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pPB9P2

Plasmid

#210030

Purpose

Contains PE2 prime editing gRNA cloning site, inducible prime editor 2, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

prime editor 2
rtta-T2A-puro

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF-1α and TRE3G

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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SOPP3-HA-nes

Plasmid

#229071

Purpose

Expresses SOPP3 in the cytosol of mammalian cells

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express SOPP3 in the cytosol of mammalian cells

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-EYFP

Plasmid

#231926

Purpose

Cre-on/Flp-on EYFP under the Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV

Promoter

Ef1a

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_HDAC1

Plasmid

#183297

Purpose

All-in-One CRISPRko system with a guide RNA that targets HDAC1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

HDAC1

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-KGRQtoSYAA-shRNAres_W

Plasmid

#147901

Purpose

Mammalian Expression of HsNot1iso1-KGRQtoSYAA-shRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1-KGRQtoSYAA-shRNAres (CNOT1 Human)

Tags

GFP (G4C mutation)

Expression

Mammalian

Mutation

NM_016284.4, K1426S, G1451Y, R1458A, Q1549A

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144~451-CMV2-mir1

Plasmid

#164664

Purpose

Express miR-144~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144~451 (MIR451A, MIR144 Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pmEmerald-ERGIC-53

Plasmid

#170717

Purpose

Expresses mEmerald-tagged human ERGIC-53 at high level in mammalian cells.

Depositor

Ke Xu

Article

Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

Insert

ERGIC-53 (LMAN1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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mCerulean3-N1

Plasmid

#54730

Purpose

Localization: N1 Cloning Vector, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Type

Empty backbone

Tags

mCerulean3

Expression

Mammalian

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pTP1183

Plasmid

#104163

Purpose

Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (1x Cysteine)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-rabbit IgG Fc specific nanobody TP897 (1xCysteine)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ApoE2

Plasmid

#197146

Purpose

Expresses ApoE2 in Mammalian cells

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

ApoE2 (APOE Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBABE GFP

Plasmid

#10668

Purpose

Empty backbone for retroviral gene expression. Select recipient cells using GFP.

Depositor

William Hahn

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

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