We narrowed to 20,388 results for: ACE;

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MTK2_015

Plasmid

#123707

Purpose

Encodes pSynTF (miniCMV core) as a Type 2 part to be used in the MTK sytem

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSynTF

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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B0032m_BC

Plasmid

#66020

Purpose

MoClo Basic Part: RBS - Weiss RBS, medium strength. Modified from Bba_B0032 to adjust spacing in MC system. [B:B0032m:C]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

RBS

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pGREAT4

Plasmid

#170892

Purpose

GoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NOSt+pNOS_Red-F_NOSt

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

E-Luc

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

c.375C>T silent mutation to remove BbsI site (…

Promoter

pG10-90

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pVH028

Plasmid

#80393

Purpose

Contains constitutive histidine kinase (pCon-Taz) with 4x SH3 scaffold domains + salicylate inducible phosphatase (Psal-CusSmut)

Depositor

Richard Murray

Article

Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

Inserts

Taz chimeric histine kinase
CusS (cusS )
NahR (nahR )

Use

Synthetic Biology

Tags

Fused by GS linkers to 4 SH3 domains

Expression

Bacterial

Mutation

Glycine 448 changed to Alanine, makes it primaril…

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pJ02B2Gm_EF

Plasmid

#66064

Purpose

MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

FACS Controls

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST15-Nedd4.2F823A

Plasmid

#165104

Purpose

bacterial expression of a GST fusion of Human Nedd4.2d F823A

Depositor

Arthur Haas

Article

Todaro et al J Biol Chem. 2018 Nov 23;293(47):18192-1

Insert

Nedd4.2d F823A (NEDD4L Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

F823A; mutation for destabilization of trimer fo…

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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MTK3_004

Plasmid

#123720

Purpose

Encodes hph (hygromycin B phosphotransferase) as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Hygro Resistance

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3_005

Plasmid

#123721

Purpose

Encodes ble (Zeocin resistance) as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Zeocin Resistance

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:sgRNA-Ds

Plasmid

#119069

Purpose

To clone sgRNA spacer sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_026

Plasmid

#123718

Purpose

Encodes crippled pSFFV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSFFVc

Expression

Mammalian

Mutation

Crippled Kozac sequence

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pFusX4-35

Plasmid

#63444

Purpose

Encodes RVD positions 10-12 in TALEN assembly

Depositor

Stephen Ekker

Article

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

NN NI NN

Use

TALEN

Mutation

none

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
MTK1_018

Plasmid

#123681

Purpose

Encodes ConL8 as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L8

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBEST_OR2-OR1-Pr_UTR1-deGFP-T500 BsaI, BbsI-safe (105p)

Plasmid

#70194

Purpose

Derived from 21 with silent mutations to make BsaI, BbsI compatible

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBTK205

Plasmid

#110587

Purpose

BTK Type 3 coding sequence for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP optim-1

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK305

Plasmid

#110594

Purpose

BTK Type 4 terminator for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

T7 terminator

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MTK5_011

Plasmid

#123855

Purpose

Encodes ConR8 as a Type 5 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector R8

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_014

Plasmid

#123706

Purpose

Encodes pUAS (miniCMV core) as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pUAS (mini CMV)

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK4_004

Plasmid

#123813

Purpose

Encodes spacer sequence as a Type 4 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_018

Plasmid

#123710

Purpose

Encodes truncated pCMV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCMV-C

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pML2(+)BR

Plasmid

#108336

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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