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pU6-pegRNA-HEK3+24

Plasmid

#140577

Purpose

Expresses a pegRNA in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

pegRNA HEK3 +24

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-DNMT1+38

Plasmid

#140578

Purpose

Expresses a pegRNA in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

pegRNA DNMT1 +38

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAC1818_pCR8-gRG6-SA

Plasmid

#118652

Purpose

Transient transfection; Expressed RG6-SA CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gRG6-SA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHS18 (mANGPTL3-Strep)

Plasmid

#109111

Purpose

Expresses StrepII-tagged mouse ANGPTL3 in mammalian cells

Depositor

Brandon Davies

Article

Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

Insert

ANGPTL3 (Angptl3 Mouse)

Tags

StrepII

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEB31 (hANGPTL8-V5-His lenti)

Plasmid

#109116

Purpose

V5 and His-tagged human ANGPTL8 in a lentiviral vector

Depositor

Brandon Davies

Article

Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

Insert

ANGPTL8 (ANGPTL8 Human)

Use

Lentiviral

Tags

6x His and V5

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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p416TEF-NYFP

Plasmid

#99553

Purpose

Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

Depositor

Liming Li

Article

Du et al Mol Cell Biol. 2010 Oct;30(19):4644-55.

Insert

Swi1 N-rich region (AA1-327) (SWI1 Budding Yeast)

Tags

YFP

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pBMN-neo-Flag-TNFR1 L380A Del229-244

Plasmid

#44098

Depositor

Martin Kluger

Article

D'Alessio et al J Biol Chem. 2010 Jul 30;285(31):23868-7

Insert

human tumor necrosis factor receptor 1 (TNFRSF1A Human)

Use

Retroviral

Tags

Flag

Mutation

L380A

Available Since

April 8, 2013

Availability

Academic Institutions and Nonprofits only
GST-Cav (135-178)

Plasmid

#22448

Depositor

William Sessa

Article

Garcia-Cardena et al J Biol Chem. 1997 Oct 10. 272(41):25437-

Insert

Caveolin 1

Tags

GST

Expression

Bacterial

Mutation

aa 135-178

Available Since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only
GST-Cav (61-101)

Plasmid

#22447

Depositor

William Sessa

Article

Garcia-Cardena et al J Biol Chem. 1997 Oct 10. 272(41):25437-

Insert

Caveolin1 (61-101)

Tags

GST

Expression

Bacterial

Available Since

Jan. 26, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-M2-TLR2-V660N

Plasmid

#12292

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I, H202Y and V660N; signal peptide (aa1-23) re…

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pBdox-MEF2C-T2A-CEBPB-P2A-IRF8_PuroR

Plasmid

#250292

Purpose

PiggyBac integration of 3 transcription factors (MEF2C, CEBPB, IRF8) under a dox-inducible promoter

Depositor

George Church

Article

Liu et al Nat Commun. 2025 Jun 10;16(1):5136. doi:

Insert

MEF2C-T2A-CEBPB-P2A-IRF8

Expression

Mammalian

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SARS2-S-D614 (NC-flag)

Plasmid

#156418

Purpose

Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

Depositor

Hyeryun Choe, Michael Farzan

Article

Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

Insert

SARS-CoV-2 S protein (S SARS CoV2)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-YE1-BE3-FNLS-CMV-mCherry

Plasmid

#154005

Purpose

Express YE1-BE3-FNLS

Depositor

Hui Yang

Article

Zuo et al Nat Methods. 2020 May 18. pii: 10.1038/s

Insert

YE1-BE3-FNLS

Use

CRISPR

Tags

FLAG

Promoter

CMV

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR207 SARS-CoV-2 ORF3A_nostop

Plasmid

#149319

Purpose

Gateway-compatible Entry vector, with insert of ORF3A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF3A (ORF3a SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR207 SARS-CoV-2 NSP1_nostop

Plasmid

#149304

Purpose

Gateway-compatible Entry vector, with insert of NSP1 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP1 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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tdTomato/pTREX-b

Plasmid

#68709

Purpose

Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.

Depositor

Roberto Docampo

Article

Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

Insert

tdTomato

Use

Expression of tdtomato red fluorescence protein i…

Available Since

Oct. 8, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pHTN-HaloTag_FBXO3:1-471

Plasmid

#211071

Purpose

Mammalian expression of full-length human FBXO3. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

FBXO3:M1-F471 (FBXO3 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBiT3.1-N_DCAF7:1-342

Plasmid

#211118

Purpose

Mammalian expression of full-length human DCAF7. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF7:M1-V342 (DCAF7 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCART0017_CROPseq-CAR-Puro_GD2-BBz

Plasmid

#242985

Purpose

GD2-BBz 14g2a CAR

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

GD2-BBz 14g2a CAR

Use

Lentiviral

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-MCL1

Plasmid

#202663

Purpose

Tet-ON 3rd generation expression vector with inducible expression of MCL1 and a puromycin selection cassette

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MCL1 apoptosis regulator, BCL2 family member (MCL1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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