We narrowed to 14,390 results for: cas9
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Plasmid#53188PurposeExpresses the S. pyogenes sgRNA from the human U6 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRExpressionMammalianPromoterhuman U6Available SinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only
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pAsp-AMA-gpdA-ptr
Plasmid#191016PurposeCRISPR vector for cloning sgDNAs to target genes of several Aspergillus species (especially section Flavi)DepositorTypeEmpty backboneUseUnspecified; AspergillusAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
p458 VRER
Plasmid#101728PurposeExpresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifsDepositorInsertSpCas9 VRER
Tags3xFLAG-NLS and NLSExpressionMammalianMutationVRER (D1135V, G1218R, R1335E, and T1337R mutation…Available SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmU6-gRNA
Plasmid#53187PurposeExpresses the S. pyogenes sgRNA from the mouse U6 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRExpressionMammalianPromotermouse U6Available SinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-hRad51
Plasmid#125570PurposeExpresses the Rad51 protein in mammalian cells under CMV promoterDepositorAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tet-inducible Luciferase reporter
Plasmid#64127PurposePhotoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.DepositorInsertluciferase
UseLuciferaseExpressionMammalianPromoterTetOx13-CMVAvailable SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV GG hUbC-dsRED
Plasmid#84034PurposeContains Golden Gate cassette to express up to four gRNAs and dsREDDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRU41
Plasmid#167663PurposeENTRY vector for gRNA1 cloning under pAtU6 promoterDepositorInsertpAtU6
UseCRISPRAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU42
Plasmid#167664PurposeENTRY vector for gRNA2 cloning under pAtU3 promoterDepositorInsertpAtU3
UseCRISPRAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKM197
Plasmid#132665PurposepyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.DepositorInsertUpstream & Downstream pyrE deletion region
UseE. coli - c. difficile shuttle vectorAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX-sgRNA
Plasmid#50662PurposeLentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)DepositorInsertsgAAVS1
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141B
Plasmid#69291PurposeGateway entry vector; single gRNA under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRAvailable SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
GAL4UAS-Luciferase reporter
Plasmid#64125PurposePhotoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.DepositorInsertGAL4UAS
UseLuciferaseTagsluciferaseExpressionMammalianPromoterGAL4UASAvailable SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
NM1-5S-tRNA-SgH
Plasmid#128178PurposeExpresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoterDepositorInsertgRNA cloning cassette
UseCRISPRExpressionYeastPromoter5S rRNA-tRNA(Arg)Available SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRU43
Plasmid#167665PurposeENTRY vector for gRNA3 cloning under pAtU6 promoterDepositorInsertpAtU6
UseCRISPRAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU44
Plasmid#167666PurposeENTRY vector for gRNA4 cloning under pAtU3 promoterDepositorInsertpAtU3
UseCRISPRAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAT30
Plasmid#204294PurposeFor marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.DepositorInsertMHC-DsRed
UseCRISPRPromoterMHCAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
U6.3>Control.gRNA.f+e
Plasmid#99140PurposeControl gRNA under the regulation of an optimized chicken specific U6 promoterDepositorInsertControl gRNA
UseCRISPRPromoterChicken U6.3Available SinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJAT11
Plasmid#204290PurposeFor inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.DepositorInsertie1-DsRed
UseCRISPRPromoterie1Available SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:SEP-GluA1 TKIT donor
Plasmid#169418PurposeDonor DNA for TKIT knockin of SEP to GluA1 in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRExpressionMammalianAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only