We narrowed to 154,128 results for: ins;
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Plasmid#210965PurposeMammalian expression of full-length human CORO1A. C-terminal NanoLuc luciferase tag.DepositorInsertCORO1A:M1-K461 (CORO1A Human)
TagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-N_DCAF1:1038-1400
Plasmid#210962PurposeMammalian expression of human DCAF1 fragment E1038 to E1400. N-terminal NanoLuc luciferase tag.DepositorInsertDCAF1:E1038-E1400 (DCAF1 Human)
TagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_FBXW11:1-542
Plasmid#210952PurposeMammalian expression of full-length human FBXW11. C-terminal NanoLuc luciferase tag.DepositorInsertFBXW11:M1-R542 (FBXW11 Human)
TagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_SKIC8:1-305
Plasmid#211038PurposeMammalian expression of full-length human SKIC8. N-terminal HiBiT tag.DepositorInsertSKIC8:M1-I305 (SKIC8 Human)
TagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_COPB2:1-597
Plasmid#211055PurposeMammalian expression of human COPB2 fragment M1 to A597. C-terminal HiBiT tag.DepositorInsertCOPB2:M1-A597 (COPB2 Human)
TagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMW363
Plasmid#238208PurposeExpresses TasR with an N-terminal Twin-StrepII tag, followed by 3xRNA1 TIGR array. AmpRDepositorInsertTaTasR
TagsTwinStrepIIExpressionBacterialAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRVL-5
Plasmid#104583PurposeContains human IgG1 CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functionsDepositorAvailable SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEM318 - sgRNA mosTI unc-119 single-copy
Plasmid#159822PurposeSingle copy or array insertion by MosTI using split unc-119 selectionDepositorInsertsgRNA mosTI unc-119 single-copy
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM272 - MCS mosTI Pmlc-2_GFP target
Plasmid#159834PurposeSingle copy or array insertion by MosTI using split Pmlc-2 GFP selectionDepositorInsertMCS mosTI Pmlc-2_GFP target
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM255 - sgRNA mosTI Pmlc-2_GFP
Plasmid#159835PurposeSingle copy or array insertion by MosTI using split Pmlc-2 GFP selectionDepositorInsertsgRNA mosTI Pmlc-2_GFP
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
CRY2olig-mCherry
Plasmid#60032PurposeExpresses a fusion of CRY2PHR E490G with mCherryDepositorAvailable SinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
BPK1520
Plasmid#65777PurposeHuman expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNADepositorInsertSpCas9 gRNA backbone, without spacer sequence
UseCRISPRExpressionMammalianPromoterU6Available SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES (M)
Plasmid#182436PurposeYeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS(M)-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-FAM134B-LIRmut
Plasmid#109027Purposestable lentiviral expression of cDNADepositorInsertFAM134B-LIRmut (RETREG1 Human)
UseLentiviralTagseGFPExpressionMammalianMutationDDFELL to DDAEAL aa 453-458 to interrupt FAM134B …PromoterEIF-1a promoterAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
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Lambda Phosphatase
Plasmid#79748PurposeIntended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.DepositorInsertLambda Phosphatase
ExpressionBacterialPromoterT7Available SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVP144
Plasmid#222081PurposeSingle component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and eforRED for plasmid eviction.DepositorInsertdCas9-PmCDA1, sfGFP, sacB and eforRED
UseCRISPRExpressionBacterialAvailable SinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChRger2-TS-EYFP
Plasmid#127237PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.DepositorInsertChRger2-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianPromoterhSyn1Available SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tn7 helper plasmid
Plasmid#141161PurposeTemperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoterDepositorInsertTn7 transposon machinery
ExpressionBacterialPromoterArabinose-inducible pBADAvailable SinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIRE4-Azi
Plasmid#105829PurposePlasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cellsDepositorInsertsHumanized EAziRS
4 copies of the U6-BstYam tRNA cassette
ExpressionMammalianMutation(Y37L, D182S, F183M, L186A, D265R)TyrRSPromoterPGK and U6Available SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only