We narrowed to 5,641 results for: pcas

1. pUDP012

   Plasmid

   #101167

   Purpose

   E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Insert

   HH-gRNA-HDV targetting SeILV6 in S. pastorianus
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry-PHR-iSH-T2A-CIBNcaax

   Plasmid

   #173860

   Purpose

   Mammalian expression of mCherry-PHR-iSH-T2A-CIBNcaax (opto-PI3K)

   Depositor

   Takao Nakata

   Article

   Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

   Insert

   mCherry-PHR-iSH-T2A-CIBNcaax
   

   Use

   Tags

   C-terminal CAAX-box and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. VRQR-HF1 variant

   Plasmid

   #138563

   Purpose

   Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   VRQR-HF1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUG-T2A-Cas9

   Plasmid

   #75346

   Purpose

   Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

   Depositor

   Bryan Luikart

   Article

   Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP (via t2a)

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330 N-FKBP-Cas9

   Plasmid

   #162727

   Purpose

   Mammalian expression

   Depositor

   Amit Choudhary

   Article

   Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

   Insert

   FKBP F36V
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. VIPR1

   Plasmid

   #51865

   Purpose

   Expresses full-length Vasoactive intestinal polypeptide receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   VIPR1 (VIPR1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. L-CRISPR-CTN (MLL-ctrl)

   Plasmid

   #69215

   Purpose

   Advanced lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; control, MLL-sgRNA + luciferase-sgRNA

   Depositor

   Dirk Heckl

   Article

   Reimer et al Haematologica. 2017 Jun 1. pii: haematol

   Inserts

   SpCas9
   Sp sgRNA scaffold
   SFFV promoter
   P2A-mNeonGreen
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRI018-pKW20088-PelcA-mCherry-TtrpC-PU3-E1-Cas9Scaffold-8xT

   Plasmid

   #140205

   Purpose

   Proof-of-concept of CRISPR/dSpCas9-VPR activation, encoding PelcA-mCherry along with the sgRNA E1 expression cassette, targeting PelcA.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Inserts

   mCherry
   sgRNA E1
   

   Use

   CRISPR and Synthetic Biology; Proof-of-concept te…

   Tags

   Expression

   Mutation

   G174D

   Promoter

   PelcA and U3

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGMC00034 (aka pMC0235)

   Plasmid

   #210751

   Purpose

   Non-targeting guide RNA

   Depositor

   Raj Chari

   Article

   Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

   Insert

   Non-targeting guide RNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Polymerase expression - pCMV-B103 (LM2907)

    Plasmid

    #208968

    Purpose

    Unfused B103 DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    B103-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. SIN-PGK-Cas9-V5-WPRE

    Plasmid

    #87876

    Purpose

    To produce lentiviral vector for Cas9 editing

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    spCas9
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Human codon-optimized

    Promoter

    mouse PGK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. myc-hRAD18 dSAP

    Plasmid

    #68830

    Purpose

    Expresses c-myc-tagged human RAD18 deleting SAP domain

    Depositor

    Satoshi Tateishi

    Article

    Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

    Insert

    c-myc tagged human RAD18 deleting SAP domain (RAD18 Human)
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

13. myc-hRAD18 dC2

    Plasmid

    #68831

    Purpose

    Expresses c-myc-tagged human RAD18 deleting Polymerase eta binding domain

    Depositor

    Satoshi Tateishi

    Article

    Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

    Insert

    c-myc tagged human RAD18 deleting Polymerase eta binding domain (RAD18 Human)
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pX459_HR_Prnp_3

    Plasmid

    #78623

    Purpose

    pX459 vector encoding SpCas9-2A-Puro and a chimeric guide RNA targeting Prnp coding sequence

    Depositor

    Walker Jackson

    Article

    Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

    Insert

    HR_Prnp_3 (Prnp Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330 NC-FKBP-Cas9

    Plasmid

    #162725

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGB3 alpha1 Pnos:PhiC31int:Tnos (GB1531)

    Plasmid

    #160616

    Purpose

    TU for the constitutive expression of Streptomyces phage PhiC31 integrase.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. CAG-RORb2

    Plasmid

    #73999

    Purpose

    Overexpression of RORb2

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    RORb2 ORF (Rorb Mouse)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. FL MALT1 Catalytic Mutant in pET29b-Strep

    Plasmid

    #48969

    Purpose

    Expresses Full length catalytic mutant MALT1 in e.coli

    Depositor

    Guy Salvesen

    Article

    Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

    Insert

    MALT1 isoform A (MALT1 Human)
    

    Use

    Tags

    His and Strep

    Expression

    Bacterial

    Mutation

    Catalytic Mutant C464A

    Promoter

    T7

    Available since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

19. myc-hRAD18 d6BD

    Plasmid

    #68832

    Purpose

    Expresses c-myc-tagged human RAD18 deleting hRAD6 binding domain

    Depositor

    Satoshi Tateishi

    Article

    Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

    Insert

    c-myc tagged human RAD18 deleting hRAD6 binding domain (RAD18 Human)
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

20. lentiGuideFB-Puro-A

    Plasmid

    #192506

    Purpose

    For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a nd a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

    Insert

    hU6-sgRNA-CS1-BsmBI-EFS-Puro-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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