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pRL648

Plasmid

#70283

Purpose

pUC plasmid with inverted polylinker flanking PpsbA-Km marker

Depositor

Peter Wolk

Article

Black et al J Bacteriol. 1994 Apr;176(8):2282-92.

Type

Empty backbone

Use

Bacterial shuttle vector

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pNJB98

Plasmid

#51520

Purpose

Gateway entry vector with attL5 and attL2 sites flanking us:NPTII repair template sequence

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Insert

us:nptII repair template

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pBN27

Plasmid

#35120

Depositor

John McCusker

Article

Hartzog et al Yeast. 2005 Jul 30;22(10):789-98.

Insert

fcy1delta1

Use

Yeast replicating

Mutation

amplicon has a complete deletion of FCY1 ORF, fla…

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pLG320

Plasmid

#124370

Purpose

Vanillic acid inducible system for expression in B. subtilis

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

gfpmut2

Expression

Bacterial

Promoter

Pspank(V)

Available Since

Jan. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pSGDlucV3.0

Plasmid

#119760

Purpose

dual luciferase with FMDV StopGo sequences flanking MCS

Depositor

John Atkins

Article

Loughran et al Mol Cell. 2022 Oct 6;82(19):3745-3749.e2

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Promoter

SV40 and T7

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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FoRC.attBGm

Plasmid

#84588

Purpose

Drosophila PhiC31 transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive GFP reporter.

Depositor

Francesca Pignoni

Article

Zhou et al Genesis. 2016 Oct 3. doi: 10.1002/dvg.22

Type

Empty backbone

Expression

Insect

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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No-repeats

Plasmid

#122487

Purpose

A control plasmid that is similar to PRExpress but lacks poly-PRE sequence. (GenBank: MF058599.1)

Depositor

Renato Paro

Article

Akmammedov et al Sci Rep. 2017 Jul 31;7(1):6899. doi: 10.

Insert

LacZ

Expression

Insect

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCJ162

Plasmid

#175055

Purpose

lox66-72 flanked gentamycin resistance cassette integrates into aquI integration site

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

lox66::gmR::lox71

Use

Unspecified

Promoter

n/a

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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HisMS2_VLP_RSV

Plasmid

#175958

Purpose

Generation of MS2 Virus-Like Particles packaged with the sequence for the RSV P and M genes [GenBank: M74568.1]

Depositor

Paul Freemont

Article

Unruh et al medRxiv 2024.04.17.24305965

Inserts

P gene
M gene

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pTXB1-sfGFP

Plasmid

#51562

Purpose

Expression of superfolder GFP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone)

Depositor

Sankar Adhya, Francisco Malagon

Insert

superfolder GFP

Tags

Intein-Chiting Binding Domanin

Expression

Bacterial

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pDonor_hU6

Plasmid

#69312

Purpose

sgRNA scaffold and human U6 promoter

Depositor

Andrea Ventura

Article

Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

Insert

hU6 promoter and sgRNA scaffold flanked by BbsI sites

Use

CRISPR

Promoter

human U6 promoter

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pSC142

Plasmid

#122835

Purpose

SUC2 promoter with GreenGate A-B flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

SUC2 promoter

Use

Golden gate compatible cloning vector

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pPEE7

Plasmid

#128357

Purpose

Recycable marker for use in Cryptococcus neoformans. Contains the amdS1 counterselectable marker flanked by direct repeats.

Depositor

James Fraser

Article

Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

Type

Empty backbone

Use

Unspecified

Promoter

ACT1

Available Since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pPEE8

Plasmid

#128358

Purpose

Recycable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by direct repeats.

Depositor

James Fraser

Article

Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

Type

Empty backbone

Use

Unspecified

Promoter

TEF1

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pNJB80

Plasmid

#51518

Purpose

Gateway entry vector with attL5 and attL2 sites flanking a CmR and ccdB gene. In our hands, the multisite reaction still proceeds efficiently.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Type

Empty backbone

Use

Gateway entry vector

Available Since

March 6, 2014

Availability

Academic Institutions and Nonprofits only

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pPL5617_pUG_PuroR

Plasmid

#60928

Purpose

PCR template vector for generating puromycin resistance cassette.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

puromycin N-Acetyltransferase

Use

Loxp flanked deletion cassette template

Promoter

TEF1

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pDG058

Plasmid

#122840

Purpose

SRDX repressor domain with GreenGate D-E flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

SRDX transcriptional repressor domain

Use

Golden gate compatible cloning vector

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMH185

Plasmid

#122839

Purpose

VP64 activator domain with GreenGate D-E flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

VP64 transcriptional activator domain

Use

Golden gate compatible cloning vector

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pNIC-TolB-NHSTIIT-GG

Plasmid

#215792

Purpose

Cloning of target gene with N terminal TolB SRP pathway signal peptide N terminal His6 Twin Strep tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-PelB-NH10T-AVITAG-GG

Plasmid

#215793

Purpose

Cloning of target gene with N terminal PelB SEC pathway signal peptide His10 and AVITAG and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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