We narrowed to 5,564 results for: chia

1. tsPurple chromoprotein promoter-less

   Plasmid

   #118007

   Purpose

   For course in "Synthetic Biology: A Lab Manual"

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   RBS, tsPurple
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBMN YFP-ltDHFR(DD)

   Plasmid

   #47080

   Purpose

   ecDHFR based destable domain function at low temperature for regulating protein stability in Caenorhabditis elegans

   Depositor

   Thomas Wandless

   Article

   Cho et al PLoS One. 2013 Aug 22;8(8):e72393. doi:

   Insert

   YFP-ltDHFR(DD)
   

   Use

   Retroviral

   Tags

   HA and YFP

   Expression

   Mutation

   amino acids mutations: R12H, N37D, N59D, G67S, D1…

   Promoter

   LTR

   Available since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSCL.180

   Plasmid

   #185001

   Purpose

   Express -Eco1 AAVS1 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
   

   Use

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   AAVS1 donor, a1/a2 length extended for 27 bp

   Promoter

   H1

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM6-RgTALsyn-HpaBC

   Plasmid

   #100949

   Purpose

   Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Inserts

   RgTALsyn
   HpaB
   HpaC
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   T7

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDGB1alpha2_SulR

   Plasmid

   #186425

   Purpose

   transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter

   Depositor

   Lukas Fischer

   Article

   Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

   Insert

   SulR
   

   Use

   Synthetic Biology; Binary vector for escherichia …

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Pnos

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCK354

   Plasmid

   #129698

   Purpose

   For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120010799

   Depositor

   John Heap

   Article

   Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

   Inserts

   PrhaBAD
   yfp
   rhaS
   Terminator ECK120010799
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. K401-Micro

   Plasmid

   #122485

   Purpose

   Component of optically-induced motor dimerization system

   Depositor

   Matt Thomson

   Article

   Ross et al Nature. 2019 Aug;572(7768):224-229. doi:

   Insert

   His tag MBP Kinesin 1-401 SspB micro
   

   Use

   Tags

   H6-MBP-TEV and SspB micro

   Expression

   Bacterial

   Mutation

   Kinesin amino acids 1-401

   Promoter

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. BAT-gal

   Plasmid

   #20889

   Depositor

   Stefano Piccolo

   Article

   Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

   Insert

   7XTCF binding sites, Siamois minimal promoter, NLS-lacz
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   7 multimerized TCF-binding elements cloned upstre…

   Promoter

   Available since

   May 7, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pCK389.AAV

   Plasmid

   #177327

   Purpose

   aTc inducible MCP-SoxS double mutant expression for CRISPRa in E.coli. Contains constitutive dCas9 and scRNA expression

   Depositor

   James Carothers

   Article

   Tickman et al Cell Syst. 2021 Nov 17. pii: S2405-4712(

   Inserts

   SoxS
   dCas9
   

   Use

   CRISPR

   Tags

   MCP (MS2 Coat Protein)

   Expression

   Mutation

   R93A,S101A

   Promoter

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQCascade-IS186

    Plasmid

    #140627

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS186. The crRNA-IS186 targets IS186 loci in Escherchia coli.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS186
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. promoter J23110 (upstream of RBS, mRFP1)

    Plasmid

    #117852

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    medium-high promoter, RBS, mRFP1
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSBK.013

    Plasmid

    #187213

    Purpose

    Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT]), and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT])
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7/Lac

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMJA104

    Plasmid

    #128520

    Purpose

    Expresses amyloid human Lysozyme in Pichia pastoris

    Depositor

    Marcos Alcocer

    Article

    Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

    Insert

    Artificial gene for human lysozyme I56T (LYZ Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSKA397

    Plasmid

    #80380

    Purpose

    mini-Tn7 delivery plasmid with metE under the cpcG2Δ59 promoter.

    Depositor

    Mustafa Khammash

    Article

    Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

    Inserts

    cat
    metE
    

    Use

    Synthetic Biology; Mini-tn7 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cat promoter and cpcG2Δ59

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Affibody EGFR:1907.BirA*-pET301/NT-DEST

    Plasmid

    #174691

    Purpose

    Plasmid encoding for the bacterial expression of a bispecific coding for the anti-EGFR affibody ZEGFR:1907 fused to the mutated form of BirA enzyme, BirA*

    Depositor

    Jean Gariepy

    Article

    Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2

    Insert

    Anti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*
    

    Use

    Tags

    HIS tag

    Expression

    Bacterial

    Mutation

    R118G mutation in BirA

    Promoter

    T7 promoter

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSBK.014

    Plasmid

    #187215

    Purpose

    Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 6 [CAG-CTG]), and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v35 (long a1/a2, barcode 6 [CAG-CTG])
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7/Lac

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAT-9208

    Plasmid

    #124221

    Purpose

    Cloning plasmid for a self-targeting gRNA library

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    self-targeting gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEA02

    Plasmid

    #172193

    Purpose

    Suicide vector carrying the site attL of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.

    Depositor

    Jean-Luc Pernodet

    Article

    Santos et al Microorganisms. 2022 Apr 16;10(4). pii:

    Insert

    attL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. aav-CAG-FLEX-tdNfsB(F124W)-mCherry (eNTR)

    Plasmid

    #113762

    Purpose

    AAV-mediated conditional expression of bacterial tdNfsB-mCherry (eNTR) under the CAG promoter

    Depositor

    Luke Lavis

    Article

    Gruber et al ACS Chem Biol. 2018 Aug 29. doi: 10.1021

    Insert

    tdNfsB(F124W)-mCherry
    

    Use

    AAV and Cre/Lox

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    F124W

    Promoter

    CAG

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQCascade-IS3

    Plasmid

    #140626

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS3. The crRNA-IS3 targets IS3 loci in Escherchia coli.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS3
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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