We narrowed to 10,337 results for: plasmids 101

1. pGuide

   Plasmid

   #64711

   Purpose

   Cloning and expression of Streptococcus pyogenes guide RNA

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Streptococcus pyogenes guide RNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. CD4-MKII-hLOV1-TEVcs(ENLYFQ/M)- Gal4

   Plasmid

   #163026

   Purpose

   FLiCRE TF for HEK293T expression

   Depositor

   Alice Ting

   Article

   Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

   Insert

   CD4-MKII-hLOV1-TEVcs-Gal4
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZac2.1-GfaABC1D -IgK-TurboID-HA

   Plasmid

   #219810

   Purpose

   Secreted HA-tagged TurboID for extracellular proximity labeling

   Depositor

   Cagla Eroglu

   Article

   Irala et al Neuron. 2024 Mar 27:S0896-6273(24)00161-

   Insert

   IgK (Igk Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-SpySrtA

   Plasmid

   #51139

   Purpose

   expression plasmid for N-terminal his tagged S.pyogenes SrtA

   Depositor

   Hidde Ploegh

   Article

   Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

   Insert

   S.pyogenes SrtA
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-80

   Promoter

   T7

   Available Since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. p12x601

   Plasmid

   #157785

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with central TetO following PEG removal of plasmid backbone

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   pMD2.G(spei/spei)&12x601_tetO
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAJT194

   Plasmid

   #202293

   Purpose

   Type IIS cloning plasmid for LPattB5 integrations w/ OriT

   Depositor

   Christopher Voigt

   Article

   Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNB22

   Plasmid

   #103059

   Purpose

   medium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the nitric oxide-inducible promoter hmpAp

   Depositor

   Dirk Bumann

   Article

   Burton et al Cell Host Microbe. 2014 Jan 15;15(1):72-

   Insert

   GFP-OVA
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   Promoter

   hmpAp

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFW2000

   Plasmid

   #224211

   Purpose

   E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin)

   Depositor

   Ophelia Venturelli

   Article

   Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-OTp-mCherry

   Plasmid

   #184754

   Purpose

   To drive mCherry under the control of mouse oxytocin promoter

   Depositor

   Kazunari Miyamichi

   Article

   Inada et al Neuron. 2022 Apr 12. pii: S0896-6273(22)

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   mouse Oxytocin promoter

   Available Since

   May 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. (SUMO)10

    Plasmid

    #126948

    Purpose

    Bacterial expression plasmid for (SUMO)10 client to assess SUMO-SIM scaffolds.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    10 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    unknown

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-ConVERGD-N2cG-WPRE

    Plasmid

    #218757

    Purpose

    Provides AND intersectional (Cre+Flp) expression of N2C glycoprotein

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    N2cG
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See Depositor Comments

    Promoter

    hSyn

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB_TRE_Z18-PDGFR_EF1a_eGFP

    Plasmid

    #186304

    Purpose

    Plasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    Z18-PDGFR
    eGFP
    

    Use

    Tags

    HA, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV2/1-CAG-Flex-FlpO-WPRE

    Plasmid

    #171690

    Purpose

    Cre-dependent expression of Flp-recombinase

    Depositor

    Tobias Rose, Mark Huebener, Tobias Bonhoeffer

    Article

    Bauer et al Neuron. 2021 Aug 4;109(15):2457-2468.e12

    Insert

    Flip-recombinase
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. scFv-IFP2.0N

    Plasmid

    #215746

    Purpose

    Expresses single chain Fragment variable (scFv) fused to N-terminal part of split IFP2.0

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    IFP2.0N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAJT195

    Plasmid

    #202294

    Purpose

    Type IIS cloning plasmid for LPattB7 integrations w/ OriT

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. TC374

    Plasmid

    #122614

    Purpose

    Prpl-28::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

    Depositor

    Tian Chi

    Article

    Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

    Insert

    rtetR-QFAD::P2A::mKate::T2A::tetR-pie1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMGS58

    Plasmid

    #135311

    Purpose

    A HygR-P2A-AID cassette to tag the protein of interest with auxin-inducible degron at the n-terminal

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    HygR-P2A-AID
    

    Use

    N terminal tagging plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. (SUMO)5

    Plasmid

    #126949

    Purpose

    Bacterial expression plasmid for (SUMO)5 client to assess SUMO-SIM scaffolds.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    5 repeats of human SUMO3 separated by (GGS)4 (SUMO3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    unknown

    Available Since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFW5000

    Plasmid

    #224214

    Purpose

    E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin), tetQ

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. eSpCas9(1.1)_No_FLAG

    Plasmid

    #79877

    Purpose

    FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Untagged eSpCas9(1.1)

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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