We narrowed to 13,270 results for: cDNA

1. pWZL-hygro-FAKY861F

   Plasmid

   #216546

   Purpose

   This retroviral plasmid expresses a mutated cDNA (dominant negative: Y861F) of human PTK2

   Depositor

   Georgia Konstantinidou

   Article

   Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

   Insert

   PTK2 (Y861F) (PTK2 Human)
   

   Use

   Retroviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   FAK dominant negative mutation Y861F

   Promoter

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWZL-hygro-FAKS732A

   Plasmid

   #216541

   Purpose

   This retroviral plasmid expresses a mutated cDNA (dominant negative: S732A) of human PTK2

   Depositor

   Georgia Konstantinidou

   Article

   Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

   Insert

   PTK2 (S732A) (PTK2 Human)
   

   Use

   Retroviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   FAK dominant negative mutation S732A

   Promoter

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWZL-hygro-FAKS910A

   Plasmid

   #216542

   Purpose

   This retroviral plasmid expresses a mutated cDNA (dominant negative: S910A) of human PTK2

   Depositor

   Georgia Konstantinidou

   Article

   Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

   Insert

   PTK2 (S910A) (PTK2 Human)
   

   Use

   Retroviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   FAK dominant negative mutation S910A

   Promoter

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW57.1 WT1 +1 ATG

   Plasmid

   #217643

   Purpose

   Dox inducible WT1 cDNA - as transcribed from +1ATG (long isoform)

   Depositor

   Constanze Bonifer

   Article

   Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

   Insert

   WT1 long isoform (WT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57.1 WT1 +378 ATG

   Plasmid

   #217644

   Purpose

   Dox inducible WT1 cDNA - as transcribed from +378ATG (short isoform)

   Depositor

   Constanze Bonifer

   Article

   Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

   Insert

   WT1 short isoform (WT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW57.1 Ex8 mut WT1

   Plasmid

   #217645

   Purpose

   Dox inducible WT1 cDNA with exon 8 mutation

   Depositor

   Constanze Bonifer

   Article

   Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

   Insert

   WT1 (WT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Frameshift exon 8

   Promoter

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW57.1 Ex9 mut WT1

   Plasmid

   #217646

   Purpose

   Dox inducible WT1 cDNA with exon 9 mutation

   Depositor

   Constanze Bonifer

   Article

   Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

   Insert

   WT1 (WT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Frameshift exon 9

   Promoter

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXS-IRES-Blast CG1358

   Plasmid

   #218541

   Purpose

   Drosophila CG1358 cDNA (human codon optimized)

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   CG1358 (HisT Fly)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459-dEGF-5’UTR

   Plasmid

   #177260

   Purpose

   A knockout vector for the dog Egf

   Depositor

   Michiyuki Matsuda

   Article

   Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

   Insert

   A gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pN1mcherry-NKD2S31A

    Plasmid

    #174332

    Purpose

    encodes human NKD2 S31A mutant cDNA with a C-terminal mCherry fusion.

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    

    Use

    Tags

    mCherry tag

    Expression

    Mammalian

    Mutation

    changed Serine 31 to Alanine

    Promoter

    CMV

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEPQD0CM0065

    Plasmid

    #177019

    Purpose

    Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    1-deoxy-D-xylulose 5-phosphate synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    transit peptide removed, amplified from cDNA, con…

    Promoter

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPBpuro-UbC-loxP-canisNrg1

    Plasmid

    #173849

    Purpose

    Encoding dog Nrg1 before Cre-mediated recombination.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A cDNA of canis Nrg1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX459-dEgf

    Plasmid

    #173700

    Purpose

    A knock-out vector for the dog Egf

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiCRISPRv2-neo-dTgfa

    Plasmid

    #173842

    Purpose

    A knockout vector for the dog Tgfa.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiCRISPRv2-puro-dEgfr

    Plasmid

    #173844

    Purpose

    A knockout vector for the dog Egfr.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Egfr gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPBpuro-UbC-canisEgf

    Plasmid

    #173845

    Purpose

    Encoding dog Egf.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A cDNA of canis EGF.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R1127Q- please see dep. comments

    Promoter

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. zebrafish SLC2A2 (zfSLC2A2)

    Plasmid

    #65103

    Purpose

    Full length SLC2A2 cDNA (Danio rerio)

    Depositor

    Josep Planas

    Article

    Castillo et al Am J Physiol Regul Integr Comp Physiol.

    Insert

    SLC2A2 (slc2a2 Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

18. LP451

    Plasmid

    #90183

    Purpose

    pFLAG-CMV2 with cDNA encoding HsNPHP4 aa residues 841-1426

    Depositor

    Lotte Pedersen

    Article

    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

    Insert

    NPHP4 (NPHP4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    aa residues 841-1426

    Promoter

    CMV

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Puro-TTL-TTF-1-HDD

    Plasmid

    #74989

    Purpose

    Human TTF-1 (NKX2-1) cDNA with the homeodomain deleted

    Depositor

    David Mu

    Article

    Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

    Insert

    TTF-1 HDD mutant (NKX2-1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Homeodomain

    Promoter

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRII-Sema4b-ISH-2kb

    Plasmid

    #68030

    Purpose

    Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Semaphorin-4B (Sema4b Mouse)
    

    Use

    Mrna in situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCRII-Sema4f-ISH-2kb

    Plasmid

    #68033

    Purpose

    Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Semaphorin-4F (Sema4f Mouse)
    

    Use

    Mrna in situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pREG(R1-R4) #BGV059

    Plasmid

    #48991

    Purpose

    Retroviral Destination vector containing AttR1-AttR4 site. Use with 4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4)

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

23. HA-HIF2α wt-pBabe-Puro

    Plasmid

    #26055

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Promoter

    Available Since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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24. pHAGE_puro

    Plasmid

    #118692

    Purpose

    Empty vector to express overexpression cDNA

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCW57.1_AOX-FLAG

    Plasmid

    #177984

    Purpose

    All-in-one dox-inducible expression of Alternative Oxidase

    Depositor

    Jessica Spinelli, David Sabatini

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    AOX
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Codon optimized cDNA for expression in human cells

    Promoter

    TRE

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pICE-FLAG-NAT10-siR-WT

    Plasmid

    #59365

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of wild-type human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Steve Jackson

    Article

    Larrieu et al Science. 2014 May 2;344(6183):527-32. do

    Insert

    human NAT10 (NAT10 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Mutations to render cDNA resistant to a siRNA

    Promoter

    CMV-tet

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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27. pICE-FLAG-NAT10-siR-G641E

    Plasmid

    #59366

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of G641E mutant of human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Steve Jackson

    Article

    Larrieu et al Science. 2014 May 2;344(6183):527-32. do

    Insert

    NAT10 NM_024662 (NAT10 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    G641E and silent mutations to render the cDNA res…

    Promoter

    CMV-tet

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TetO-CDK5RAP2

    Plasmid

    #202840

    Purpose

    Doxycycline inducible expression of CDK5RAP2 cDNA

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    CDK5RAP2 (CDK5RAP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available Since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEGFP-N1_MeCP2(WT)

    Plasmid

    #110186

    Purpose

    Expression of mouse MeCP2 (WT) in mammalian cells

    Depositor

    Adrian Bird

    Article

    Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

    Insert

    MeCP2_e2 FL (mouse cDNA) (Mecp2 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GFP-GLT1b-V5

    Plasmid

    #162518

    Purpose

    Rat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loop

    Depositor

    Josef Kittler

    Article

    Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

    Insert

    GLT1b (Slc1a2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge