We narrowed to 6,203 results for: 338

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pCellFree_G03 TFAP4

Plasmid

#67073

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFAP4 (TFAP4 Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

P338A

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
EGFP-Rab7A T22N

Plasmid

#28048

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab7A (RAB7A Human)

Tags

2xHA and EGFP

Expression

Mammalian

Mutation

T22N

Available Since

May 27, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniGFP1-P2A-FusionRed

Plasmid

#188732

Purpose

Express miniGFP1 and FusionRed in mammalian cells

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP1

Use

AAV

Tags

FusionRed

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab7A Q67L

Plasmid

#28049

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab7A (RAB7A Human)

Tags

2xHA and EGFP

Expression

Mammalian

Mutation

Q67L

Available Since

May 27, 2011

Availability

Academic Institutions and Nonprofits only

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Flag-Rab7A Q86L

Plasmid

#28050

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab7A (RAB7A Human)

Tags

2xHA and 3xFlag

Expression

Mammalian

Mutation

Q86L

Available Since

June 22, 2011

Availability

Academic Institutions and Nonprofits only
pGY53

Plasmid

#221709

Purpose

Empty Cas12a vector for multiplex gene editing in fungi

Depositor

Kyle Pomraning

Article

Yuan et al Front Bioeng Biotechnol. 2024 Oct 16;12:

Type

Empty backbone

Use

CRISPR; Aspergillus

Expression

Bacterial

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET-APOBEC1-YTH

Plasmid

#200158

Purpose

For bacterial expression of the APOBEC1-YTH fusion protein for protein purification

Depositor

Kate Meyer

Article

Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

Insert

APOBEC1-YTH

Tags

6xHis, HA, and Maltose Binding Protein

Expression

Bacterial

Mutation

Codon optimized for E coli expression

Promoter

LacZ

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pEff

Plasmid

#97423

Purpose

Transient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.

Depositor

Nikolai Ravin

Article

Mardanova et al Front Plant Sci. 2017 Feb 28;8:247. doi:

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pKL2299A

Plasmid

#222105

Purpose

A ternary vir helper plasmid that carries Agrobacterium tumefaciens virulence genes for enhanced plant genetic transformation.

Depositor

Kan Wang

Article

Aliu et al Front Plant Sci. 2024 Jul 23;15:1429353.

Insert

virA

Expression

Bacterial and Plant

Promoter

virA promoter

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET-APOBEC1-YTHmut

Plasmid

#200159

Purpose

For bacterial expression of the APOBEC1-YTHmut fusion protein for protein purification

Depositor

Kate Meyer

Article

Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

Insert

APOBEC1-YTHmut

Tags

6xHis, HA, and MBP

Expression

Bacterial

Mutation

Codon optimized for E coli expression

Promoter

LacZ

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-EGFP-WPRE-pA

Plasmid

#37084

Purpose

Cre-dependent expression of EGFP

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

EGFP

Use

AAV and Cre/Lox; Cre-on

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pYL-kasOp-FnCas12a2

Plasmid

#177874

Purpose

Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

Depositor

Yunzi Luo

Article

Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

Insert

Streptomyces codon optimized FnCas12a

Use

CRISPR

Promoter

kasOp*

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Plasmid L5 attB::Pleft* mCherry

Plasmid

#169411

Purpose

Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mCherry

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

pLeft
mCherry

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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hSyn1-sypHy-RGECO

Plasmid

#84078

Purpose

Combined fluorescent reporter for both presynaptic calcium influx and vesicle excoytosis

Depositor

Juan Burrone

Article

Jackson et al Front Synaptic Neurosci. 2016 Jul 26;8:2

Insert

sypHy-RGECO

Expression

Mammalian

Promoter

hSyn1

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pGY18

Plasmid

#221708

Purpose

Empty Cas9 vector for multiplex gene editing in fungi

Depositor

Kyle Pomraning

Article

Yuan et al Front Bioeng Biotechnol. 2024 Oct 16;12:

Type

Empty backbone

Use

CRISPR; Aspergillus

Expression

Bacterial

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pEC3105 (pSpy1C-PxylS2_ffluc)

Plasmid

#218512

Purpose

replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Mutation

deletion of Plac_mrfp cassette

Promoter

PxylS2

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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epi-BE4max-NG

Plasmid

#135977

Purpose

Expresses AncBE4max(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

Depositor

Yongming Wang

Article

Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

Type

Empty backbone

Expression

Mammalian

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only
px459 sgFIP200

Plasmid

#175024

Purpose

CRISPR-Cas9 plasmid targeting exon 3 of human FIP200.

Depositor

David Tumbarello

Article

Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

Insert

RB1CC1 (RB1CC1 Human)

Expression

Mammalian

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniGFP2-P2A-FusionRed

Plasmid

#188734

Purpose

Express miniGFP2 and FusionRed in mammalian cells

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP2

Use

AAV

Tags

FusionRed

Expression

Mammalian

Promoter

CAG

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pUB-Cas9

Plasmid

#86556

Purpose

For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)

Depositor

Andreas Weber

Article

Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

Insert

Cas9

Use

CRISPR

Expression

Plant

Mutation

Cas9 is codon optimized for C. reinhardtii

Promoter

UBIQUITIN10

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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