We narrowed to 11,735 results for: pcdna

1. pcDNA3.1-MS2-HA-HsRck_296-472_AC

   Plasmid

   #148419

   Purpose

   Mammalian Expression of HsRck_296-472

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   HsRck_296-472 (DDX6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-PARL-D319N-FLAG C-t

   Plasmid

   #191957

   Purpose

   Expression of Presenilin Associated Rhomboid Like (PARL) D319N mutant

   Depositor

   Luca Scorrano

   Insert

   PARL D319N (PARL Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D319N

   Promoter

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-DEST47-Noa1-GFP

   Plasmid

   #191968

   Purpose

   Gateway Destination Vector, containing NOA1-GFP chimera coding sequence, for Gateway Cloning Technology

   Depositor

   Luca Scorrano

   Insert

   Noa1-GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO_Cep41-STf

   Plasmid

   #182010

   Purpose

   CMV overexpression of SNAP-tag fast in mammalian cell lines associated to microtubules

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   Cep41-STf
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO_H2B-STf

   Plasmid

   #181998

   Purpose

   CMV overexpression of SNAP-tag fast fusion to H2B for nuclear localization in mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   H2B-STf
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO_b4g-STf

   Plasmid

   #182005

   Purpose

   CMV overexpression of SNAP-tag fast in the golgi apparatus of mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   b4g-STf
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-HNF1B-FUS-IDR

   Plasmid

   #183240

   Purpose

   Expression of HNF1B fused to a intrinsically disordered regions of the FUS protein

   Depositor

   Jorge Ferrer

   Article

   Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

   Insert

   HNF1 homeobox B (HNF1B Human)
   

   Use

   Tags

   Fusion to an IDR from the FUS protein and FLAG ta…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-HNF1A-delta-IDR2

   Plasmid

   #183238

   Purpose

   Expression of FLAG tagged HNF1A with deletion of a intrinsically disordered regions (AA545-573)

   Depositor

   Jorge Ferrer

   Article

   Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

   Insert

   HNF1 homeobox A (HNF1A Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 545-573

   Promoter

   CMV

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.2-V5-DEST-hSFRP1-GST

   Plasmid

   #189543

   Purpose

   Protein expression of SFRP1 in mammalian cells.

   Depositor

   Haian Fu

   Insert

   secreted frizzled related protein 1 (SFRP1 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(-) HuTF-Fc-His tag

    Plasmid

    #183253

    Purpose

    Soluble expression of the extracellular domains of human tissue factor (TF)-Fc

    Depositor

    Alexander McLellan

    Insert

    Tissue factor (F3 Human)
    

    Use

    Tags

    8xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-PKAcat(G1A)-mCherry

    Plasmid

    #181852

    Purpose

    mCherry-tagged PKA catalytic subunit lacking N-terminal myristoylation site; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    PKAcat-mCherry (PRKACA Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Glycine residue immediately after start site in P…

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-NLS-YFP-CCTc

    Plasmid

    #184327

    Purpose

    Mammalian expression of the nuclear localized YFP-CCTd(peptide of Cav1.2)

    Depositor

    Xiaodong Liu

    Article

    Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

    Insert

    CCTc (CACNA1C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)-FluoSTEP-AKAR (mScarlet-I)

    Plasmid

    #181861

    Purpose

    FluoSTEP-AKAR using mScarlet-I as the FRET acceptor.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-AKAR (mScarlet-I)
    

    Use

    Tags

    mScarlet-I and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.3-hAP1 420-440

    Plasmid

    #183643

    Purpose

    To (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expression

    Depositor

    Sheng-cai Lin

    Article

    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

    Insert

    ATP6AP1 (ATP6AP1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    truncated ATP6AP1 mutant; contains only the trans…

    Promoter

    CMV

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-Prom1 Del EC2

    Plasmid

    #184025

    Purpose

    Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 273-287.

    Depositor

    Peter Stoilov

    Article

    Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

    Insert

    Prom1 SV8(-Ex19a) Del EC2 (Prom1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of exon 19a (amino acids 696-701), and d…

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-Prom1 SV8 (-Ex19a)

    Plasmid

    #184012

    Purpose

    Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701).

    Depositor

    Peter Stoilov

    Article

    Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

    Insert

    Prom1 SV8(-Ex19a) (Prom1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of exon 19a (amino acids 696-701).

    Promoter

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA T7-Gnat1 Mut-3UTR

    Plasmid

    #184027

    Purpose

    Expresses mouse Gnat1 protein with N-terminal T7 tag from cDNA that contains the mutant 3'-UTR where the TAG binding sites for MSI1 are mutated to TGA

    Depositor

    Peter Stoilov

    Article

    Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

    Insert

    Gnat1 (Gnat1 Mouse)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    TAG sites in the 3'-UTR are mutated to TGA

    Promoter

    CMV

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1_Hygro(+)_GRHL1-R9A-L3F6H

    Plasmid

    #177767

    Purpose

    Overexpression of 3xFLAG-6xHis tagged human GRHL1 (R9A mutation)

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R9A

    Promoter

    Available since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1_Hygro(+)_GRHL1-S95A-L3F6H

    Plasmid

    #177768

    Purpose

    Overexpression of 3xFLAG-6xHis tagged human GRHL1 (S95A mutation)

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S95A

    Promoter

    Available since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1_Hygro(+)_GRHL1-K116A-L3F6H

    Plasmid

    #177769

    Purpose

    Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K116A mutation)

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K116A

    Promoter

    Available since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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