We narrowed to 146,921 results for: ins;

1. pDV02_linear_entry_TagBFP-2A-MCS-2A-mNeonGreen

   Plasmid

   #200401

   Purpose

   Entry vector for open-loop ADAR-based RNA sensors. Expresses TagBFP constitutively. Expresses mNeonGreen upon target detection

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   Open-loop DART VADAR sensor entry cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AICSDP-078:G3BP1-mEGFP

   Plasmid

   #193920

   Purpose

   Homology arms and mEGFP-SGLRS for N-terminus tagging of human G3BP stress granule assembly factor 1

   Depositor

   Allen Institute for Cell Science

   Insert

   G3BP1 Homology Arms with mEGFP-SGLRS (G3BP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available Since

   Dec. 22, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. p3xFlag-CMV7-Erk2_KR

   Plasmid

   #39224

   Depositor

   Melanie Cobb

   Article

   Robinson et al J Biol Chem. 2002 Apr 26;277(17):14844-5

   Insert

   Erk2(K52R) (Mapk1 Rat)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   K52R

   Promoter

   CMV

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pVk7-33G1-BioLHis

   Plasmid

   #58949

   Purpose

   backbone for mouse recombinant antibody with added C-terminal biotinylation site, linker, 6His tag

   Depositor

   Gavin Wright

   Article

   Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

   Type

   Empty backbone

   Use

   Tags

   6His tag and biotinylation peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a(+) 6xHis-TEV-IFIT1

   Plasmid

   #53557

   Purpose

   bacterial expression of IFIT1

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT1 (IFIT1 Human)
   

   Use

   Tags

   6xHIS and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 28, 2014

   Availability

   Academic Institutions and Nonprofits only

6. shCDK4/6(1)

   Plasmid

   #73554

   Purpose

   vector encoding both shRNA against CDK4(1) and shRNA against CDK6(1)

   Depositor

   Gerardo Ferbeyre

   Article

   Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

   Insert

   shRNA against CDK4 + shRNA against CDK6
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pBC155

   Plasmid

   #202274

   Purpose

   Level 2 for strain-specific barcode DNA tag with bc182 through Tn7 bacterial insertion, Spectinomycin and Streptomycin selectable marker and tagBFP fluorescent marker

   Depositor

   Paul Schulze-Lefert

   Article

   Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

   Insert

   bc182
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBC114

   Plasmid

   #202276

   Purpose

   Level 2 for strain-specific barcode DNA tag with bc141 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum

   Depositor

   Paul Schulze-Lefert

   Article

   Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

   Insert

   bc141
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQCXIH-PSM-Rb

   Plasmid

   #37106

   Depositor

   Joseph Nevins

   Article

   Angus et al Oncogene. 2012 Jan 16. doi: 10.1038/onc.

   Insert

   Rb (RB1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   aa 379-928 only; large pocket of human Rb with aa…

   Promoter

   CMV

   Available Since

   Sept. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

10. MSP977

    Plasmid

    #65774

    Purpose

    Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135E mutation in Cas9

    Promoter

    CMV & T7

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Vh-HA-3xFKBP

    Plasmid

    #20222

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    3xFKBP (FKBP1A Human)
    

    Use

    Tags

    HA and Vh

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

12. shCDK4/6(2)

    Plasmid

    #73555

    Purpose

    vector encoding both shRNA against CDK4(2) and shRNA against CDK6(2)

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    shRNA against CDK4 + shRNA against CDK6
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2

    Plasmid

    #182358

    Purpose

    Positive control for mosTI(unc-119). unc-119 rescue and germline nuclear GFP expression after mosTI insertion.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Pmex-5

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJ23119-sgRNA

    Plasmid

    #113654

    Purpose

    sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

    Depositor

    Jeffrey Barrick

    Article

    Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

    Insert

    promoter J23119 and sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PKG I alpha dNPS

    Plasmid

    #16391

    Depositor

    Bernard Weinstein

    Article

    Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

    Insert

    PKG1 alpha (PRKG1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N-terminal pseudosubstrate domain deletion: delet…

    Promoter

    Available Since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pSCM167-Venus-VF-hADAM10(aa220-654)

    Plasmid

    #189560

    Purpose

    Protein expression ADAM10 in mammalian cells. Contains metalloprotease, disintegrin, and cysteine rich domains--amino acids 220-654

    Depositor

    Haian Fu

    Insert

    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    contains aa 220-654

    Promoter

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Vh-HA-FKBP

    Plasmid

    #20220

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    FKBP (FKBP1A Human)
    

    Use

    Tags

    HA and Vh

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

18. MSP2262

    Plasmid

    #70703

    Purpose

    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    D10A & H557A in SaCas9

    Promoter

    T7

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNP1 eforRed toehold switch

    Plasmid

    #107353

    Purpose

    Expresses eforRed (an RFP derivative) in Ecoli off a T7 promoter when pNP1 eforRed trigger is co-expressed

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold switch 1 + eforRed
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFUGW-ZipACR-EYFP

    Plasmid

    #88844

    Purpose

    Fast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spiking

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    ZipACR-NotI-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge