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BLBP-GFP

Plasmid

#63174

Purpose

Expressing GFP from BLBP promoter which is a neural stem cell (radial glial cells) specific promoter

Depositor

Bart De Strooper

Article

Shariati et al J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77

Insert

BLBP (Fabp7 Mouse)

Expression

Mammalian

Promoter

1700bp upstream of BLBP gene

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pInducer20 TIFA T9A

Plasmid

#231590

Purpose

TIFA gene mutated in position T9A (mutation on phosphorylation site) under control of a doxycycline-inducible promoter

Depositor

Thomas Henry

Article

Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

Insert

TIFA (TIFA Human)

Use

Lentiviral

Tags

3X flag

Expression

Mammalian

Mutation

T9A

Promoter

doxycycline

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pInducer20 ALPK1 WT

Plasmid

#231585

Purpose

ALPK1 WT gene under control of a doxycycline-inducible promoter

Depositor

Thomas Henry

Article

Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

Insert

ALPK1 (ALPK1 Human)

Use

Lentiviral

Tags

3X flag

Expression

Mammalian

Promoter

doxycycline

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-RHO-IntC-dCas9C-VPR-polyA

Plasmid

#166692

Purpose

Express C-terminal part of split dCas9-VPR

Depositor

Stylianos Michalakis

Article

Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

Insert

IntC-dCas9C-VPR

Use

AAV and CRISPR

Expression

Mammalian

Promoter

short human rhodopsin promoter

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3

Plasmid

#89975

Purpose

Expresses GFP-tagged ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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Flag Depdc5 pRK5

Plasmid

#46340

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Depdc5 (DEPDC5 Human)

Tags

Flag

Expression

Mammalian

Mutation

Differs from Depdc5 isoform 1 by 10 amino acids …

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

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pHIV-INK4A-zsGreen

Plasmid

#110728

Purpose

Mammalian Expression of p16INK4A IRES zsGreen

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

p16INK4A IRES zsGreen (CDKN2A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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H11-mScarletSIIN

Plasmid

#172438

Purpose

Hipp11 targeting vector, expresses mScarlet-SIINFEKL

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Insert

mScarlet-SIIN

Use

Mouse Targeting

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ER-mRFP

Plasmid

#231176

Purpose

Expresses control plasmid for ER-mitochondrial linkers, tagged with mRFP, in mammalian cells

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

ER-mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV_EF1aUBA3_IRES_Puro

Plasmid

#235660

Purpose

human UBA3 cloned into pLV-EF1a-IRES-Puro vector (Addgene: 85132)

Depositor

Lorenz Studer

Article

Saurat et al Cell Stem Cell. 2024 Aug 1;31(8):1162-11

Insert

UBA3 (UBA3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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EphA2-MT

Plasmid

#108851

Purpose

Encodes for human EphA2 fluorescently labeled with mTurquoise on the C-Terminus via a 15 amino acid (GGS)5 flexible linker

Depositor

Kalina Hristova

Article

Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.

Insert

EPHA2 (EPHA2 Human)

Tags

Labeled with mTurquoise on the C-Terminus via a 1…

Expression

Mammalian

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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TPC2(N257A)-mCherry

Plasmid

#135186

Purpose

Pore-dead mutant of TPC2, tagged on C terminus with mCherry.

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TPC2 (Tpcn2 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Mutation of asparagine257 to alanine

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pNCST-AvicFP4

Plasmid

#129507

Purpose

Expresses AvicFP4 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AvicFP4

Expression

Bacterial

Mutation

One of two variants of AvicFP4 tested with essent…

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-30nmEML-mRFP

Plasmid

#231179

Purpose

Stabilizes the ER-mitochondrial distance at 30 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

30nmEML-mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCas-dCas12m

Plasmid

#192275

Purpose

Encodes MmdCas12m (D485A) under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmdCas12m

Use

CRISPR

Expression

Bacterial

Mutation

D485A

Promoter

PJ23108

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHE111-3xFlag-HSP70

Plasmid

#236055

Purpose

Mammalian expression of human HSP70 with a N-terminal 3x-FLAG tag

Depositor

Jonathan Schisler

Article

Stewart et al bioRxiv 2025.02.17.637997

Insert

HSP70 (HSPA1A Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-RiboL1-jGCaMP8f

Plasmid

#169249

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8f protein.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSyn

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221 MLK1

Plasmid

#60540

Purpose

Donor vector for human MLK1

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

MLK1 (MAP3K9 Human)

Use

Gateway donor vector

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pStreptagII 3C SMO

Plasmid

#234995

Purpose

Expression of a Strep-tag II labeled on its N-terminus receptor, with the Smoothened (Smo) receptor

Depositor

Monica Gonzalez-Magaldi, Daniel Leahy

Article

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

SMO (SMO Human)

Tags

Streptag-II, HRV 3C site

Expression

Mammalian

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pEABR NA 3C FLAG SA

Plasmid

#234987

Purpose

For production of Extracellular Vesicles (EVs), with the transmembrane region of Neuraminidase protein fused to Streptavidin on the vesicles surface

Depositor

Monica Gonzalez-Magaldi, Daniel Leahy

Article

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

EABR-NA

Tags

HRV 3C site, FLAG, Strep-Tactin (SA)

Expression

Mammalian

Mutation

In the Streptavidin coding sequence Glu44Val/Ser4…

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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