We narrowed to 7,470 results for: addgene/1000

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pT7-EGFP-C1-HseIF4G1-Y619AL624AL625A_AD

Plasmid

#148522

Purpose

Mammalian Expression of HseIF4G1-Y619AL624AL625A

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1-Y619AL624AL625A (EIF4G1 Human)

Expression

Mammalian

Mutation

one non silent mutation M432V (natural variant is…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Cas9_ANKRD1_sgRNA2

Plasmid

#186669

Purpose

Plasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

ANKRD1 KO sgRNA Plasmid (ANKRD1 Human)

Expression

Mammalian

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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CIRC

Plasmid

#186343

Purpose

Contains VEGFA exon 7 and part of intronic flanking regions enabling expression of hsa_circ_0076611 (circVEGF)

Depositor

Giovanni Blandino

Article

Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi:

Insert

VEGFA (VEGFA Human)

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-PKAcat(G1A)-mCherry

Plasmid

#181852

Purpose

mCherry-tagged PKA catalytic subunit lacking N-terminal myristoylation site; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

PKAcat-mCherry (PRKACA Human)

Tags

mCherry

Expression

Mammalian

Mutation

Glycine residue immediately after start site in P…

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pME-MmCav1

Plasmid

#170527

Purpose

Entry clone for tagging MmCav1 at the C terminus. Parton lab clone BCJ

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Caveolin1 (Cav1 Mouse)

Use

Multisite gateway entry clone

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGMC00003

Plasmid

#166720

Purpose

sgRNA against mouse Kitl

Depositor

Raj Chari

Article

Rice et al Nat Commun. 2018 Nov 30;9(1):5099. doi:

Insert

Kitl (Kitl Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGMC00004

Plasmid

#166721

Purpose

sgRNA against mouse Kitl

Depositor

Raj Chari

Article

Rice et al Nat Commun. 2018 Nov 30;9(1):5099. doi:

Insert

Kitl (Kitl Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-f1(+)-ACTB-iTagRFP-T

Plasmid

#115915

Purpose

donor to insert iTagRFP-T at the ACTB locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

ACTB-TagRFP-T (ACTB Human)

Use

Donor template

Tags

TagRFP-T

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-H3/S3

Plasmid

#124627

Purpose

Chimeric ETS domain: H3/S3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 237 to 241 replaced with corresponding s…

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S824E-S828E

Plasmid

#91999

Purpose

Expresses FLAG-HA-AGO2 (S824E-S828E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E, S828E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S824E-S831E

Plasmid

#92000

Purpose

Expresses FLAG-HA-AGO2 (S824E-S831E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E, S831E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S828E-S831E

Plasmid

#92001

Purpose

Expresses FLAG-HA-AGO2 (S828E-S831E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S828E, S831E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S824E-S828E-S831E

Plasmid

#92002

Purpose

Expresses FLAG-HA-AGO2 (S824E-S828E-S831E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E, S828E, S831E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S824A-T830A

Plasmid

#92003

Purpose

Expresses FLAG-HA-AGO2 (S824A-T830A)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824A, T830A

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-ETS1

Plasmid

#98621

Purpose

Gateway entry TOPO TA cloning vector for ETS1

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ETS1 (Ets1 Mouse)

Use

Topo ta cloning vector

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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mIFP12-Caveolin-C-10

Plasmid

#56247

Purpose

Localization: Membrane, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Caveolin (CAV1 Human)

Tags

mIFP12

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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NKP40 RFP (794)

Plasmid

#62006

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

C17orf32 (C17orf32 Human)

Tags

mRFP

Expression

Mammalian

Promoter

pgk

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pscAAV- EF1α core -hKCNJ2-HA tag

Plasmid

#246995

Purpose

Can be used to generate AAV virus that will express human KCNJ2 with Ala115 HA tag from EF1α core promoter

Depositor

Ting Chen

Article

Chen et al Cell. 2025 Aug 14:S0092-8674(25)00857-8.

Insert

hKCNJ2 (KCNJ2 Human)

Use

AAV

Tags

HA tag (Internal at Ala115)

Promoter

EF1α

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pENTR221-BCL2

Plasmid

#251695

Purpose

Gateway-adapted BCL2 ORF

Depositor

Boyi Gan

Article

Lei et al Cancer Cell. 2025 Jun 9;43(6):1076-1092.

Insert

BCL2 BCL2 apoptosis regulator (BCL2 Human)

Use

Gateway donor vector

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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SS-Cyclin D3

Plasmid

#215116

Purpose

Expresses SS-Cyclin D3 in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND3 (CCND3 Human)

Tags

SS (STREP-STREP)

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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