We narrowed to 12,952 results for: sequence

1. D2_Ld_030320

   Plasmid

   #135818

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   DDRGK domain containing protein, putative
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. D1_Ld_290860

   Plasmid

   #135817

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   cysteine peptidase C (CPC)
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. D14_Ld_051070

   Plasmid

   #135830

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   Legume-like lectin family, putative
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. D10_Ld_292250

   Plasmid

   #135826

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   C-8 sterol isomerase-like protein
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. D17_Ld_090870

   Plasmid

   #135833

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   hypothetical protein, conserved
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRS424-ysg(G:H)

   Plasmid

   #138257

   Purpose

   Expression of GFP-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequence to be used in yeast dual reporter system

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   GFP targeting sgRNAs G and H
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19-hsg(G:H)

   Plasmid

   #138259

   Purpose

   Expression of humanized mCherry-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequences to be used in Traffic Light reporter system

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   mCherry targeting sgRNAs G and H
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. D8_Ld_340400

   Plasmid

   #135824

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   hypothetical protein, unknown function
   

   Use

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. actc1b-GalT-mKate2

   Plasmid

   #109506

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to the N-terminal sequence from zebrafish 1,4 galactosyltransferase 1 (GalT). Marks the Golgi complex.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   actc1b-GalT-mKate2
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p5E-161K enhancer-NR2F2-MUT

    Plasmid

    #138371

    Purpose

    Gateway compatible 5' entry clone contains human modified 161k enhancer sequence. with NR2F2 binding sites mutated

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    161k human enhancer with conserved NR2F2 binding sites mutated
    

    Use

    Gateway compatible

    Tags

    Expression

    Mutation

    NR2F2 sites mutated

    Promoter

    none

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBTK224

    Plasmid

    #110590

    Purpose

    BTK Type 3 coding sequence for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    E2-Crimson
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF1a-T2-GpNLuc

    Plasmid

    #135949

    Purpose

    Expresses the GpNLuc LumiFluor targeted to the mitochondira in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    GpNLuc LumiFluor
    

    Use

    Tags

    T2 mitochondrial localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF1a-T2-OgNLuc

    Plasmid

    #135951

    Purpose

    Expresses the OgNLuc LumiFluor targeted to the mitochondira in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    OgNLuc LumiFluor
    

    Use

    Tags

    T2 mitochondrial localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MTK4a_008

    Plasmid

    #123822

    Purpose

    Encodes Golgi Targeting Sequence as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    GTS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. phNFL-P22S

    Plasmid

    #132591

    Purpose

    Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E P22S mutation

    Depositor

    Anthony Brown

    Article

    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

    Insert

    NEFL (NEFL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    64C>A, 65C>G; Pro22Ser

    Promoter

    CMV

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHBS1507 [IBB-GFP-mCherry 3E]-[BFP-TDP43 VLIM-S]

    Plasmid

    #133331

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All VLIM to S in CTD of full-length TDP43

    Promoter

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHBS1508 [IBB-GFP-mCherry 3E]-[BFP-TDP43 VLIM-F]

    Plasmid

    #133332

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All VLIM to F in CTD of full-length TDP43

    Promoter

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFA0057

    Plasmid

    #131784

    Purpose

    Guide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.

    Depositor

    Frank Albert

    Article

    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

    Insert

    20mer GFP cassette guide (gGFP) and 5' sgRNA
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTAMACHis6_PiuD

    Plasmid

    #128953

    Purpose

    expresses PiuD in E.coli

    Depositor

    James Naismith

    Article

    Luscher et al Antimicrob Agents Chemother. 2018 May 25

    Insert

    Putative outer membrane receptor for iron transport (PiuD)
    

    Use

    Tags

    6xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-105)

    Promoter

    T7

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHBS1399 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G368W+G385W]

    Plasmid

    #118813

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G368W and W385G in CTD of full-length TDP43

    Promoter

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHBS1401 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 F-S]

    Plasmid

    #118808

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All F to S in CTD of full-length TDP43

    Promoter

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHBS1404 [IBB-GFP-mCherry 3E]-[BFP-P2A-TDP43 VLIM-S]

    Plasmid

    #118809

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All VLIM to S in CTD of full-length TDP43

    Promoter

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHBS1405 [IBB-GFP-mCherry 3E]-[BFP-P2A-TDP43 VLIM-F]

    Plasmid

    #118810

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All VLIM to F in CTD of full-length TDP43

    Promoter

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pVC-Ds-DrU6a:scrambled1-sgRNA-Ds

    Plasmid

    #119070

    Purpose

    Construct with scrambled-1 sgRNA sequence for microinjections in zebrafish

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:scrambled1-sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish U6a promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pVC-Ds-DrU6a:scrambled2-sgRNA-Ds

    Plasmid

    #119071

    Purpose

    Construct with scrambled-2 sgRNA sequence for microinjections in zebrafish

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:scrambled2-sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish U6a promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pVC-Ds-DrU6a:scrambled3-sgRNA-Ds

    Plasmid

    #119072

    Purpose

    Construct with scrambled-3 sgRNA sequence for microinjections in zebrafish

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:scrambled3-sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish U6a promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pVC-Ds-DrU6a:scrambled4-sgRNA-Ds

    Plasmid

    #119073

    Purpose

    Construct with scrambled-4 sgRNA sequence for microinjections in zebrafish

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:scrambled4-sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish U6a promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pT2K-CAGGS-U6-sgRNA-M7-IRES-CFP

    Plasmid

    #114730

    Purpose

    sgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc gene

    Depositor

    Martine Roussel

    Article

    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

    Insert

    sgRNA M7
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTEI041

    Plasmid

    #105414

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    Hormone-binding domain and regulatory sequences (estrogen receptor)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI/ BpiI restriction sites removed

    Promoter

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDNA-hTtAgo

    Plasmid

    #104383

    Purpose

    human codon optimized TtAgo; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al PLoS One. 2018 Mar 27;13(3):e0193818. do

    Insert

    hTtAgo
    

    Use

    Tags

    3XFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge