We narrowed to 9,085 results for: mel

1. pUBC_HA_PP7-mCherry

   Plasmid

   #107605

   Purpose

   Transient expression of non-aggregating delta-FG mutant, fused to mCherry in mammalian cells, under a hUBC promoter, HA tag

   Depositor

   John Rinn

   Article

   Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

   Insert

   PP7-mCherry
   

   Use

   Synthetic Biology

   Tags

   mCherry

   Mutation

   PP7: [delta]67_74 (non-aggregating)

   Promoter

   hUBC

   Available Since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFUGW-ZipACR-EYFP

   Plasmid

   #88844

   Purpose

   Fast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spiking

   Depositor

   John Spudich

   Article

   Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

   Insert

   ZipACR-NotI-EYFP
   

   Use

   Lentiviral

   Tags

   EYFP

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti_CRISPRi_sgNeg1

   Plasmid

   #166998

   Purpose

   Lentiviral expression of negative control sgRNA for CRISPRi knockdown

   Depositor

   Gian-Paolo Dotto

   Article

   Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

   Insert

   Negative control sgRNA 1
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. GST-MAGE-2-429

   Plasmid

   #89129

   Purpose

   bacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sites

   Depositor

   Elizabeth Wilson

   Article

   Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

   Insert

   MAGE-A11 (MAGEA11 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

5. FLAG-GAS41

   Plasmid

   #15428

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   GAS41 (Yeats4 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pCMV-BE4-RrA3F F130L

   Plasmid

   #138341

   Purpose

   Mammalian expression plasmid for BE4-RrA3F F130L

   Depositor

   Nicole Gaudelli

   Article

   Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

   Insert

   BE4-RrA3F F130L
   

   Use

   Synthetic Biology

   Tags

   BP-NLS

   Promoter

   CMV

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-GW182delta1

   Plasmid

   #11592

   Depositor

   Edward Chan

   Article

   Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

   Insert

   GW182 (TNRC6A Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains only aa1-498; SEE DEPOSITOR NOTES BELOW

   Available Since

   June 30, 2006

   Availability

   Academic Institutions and Nonprofits only

8. SNF-5 pXOOM

   Plasmid

   #48697

   Purpose

   Expressed SNF-5 in Xenopus oocytes or mammalian cells

   Depositor

   Dmitri Boudko

   Article

   Metzler et al J Exp Biol. 2013 Aug 1;216(Pt 15):2843-5

   Insert

   snf-5 (snf-5 Nematode)
   

   Use

   Xenopus oocyte expression

   Expression

   Mammalian

   Mutation

   K42E (when compared to NP_496735.1)

   Promoter

   T7

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459-sg-mGreenLantern/EGFP

   Plasmid

   #179913

   Purpose

   All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   mGreenLantern/EGFP sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGK:TRL-EFS:iRFP670

    Plasmid

    #68475

    Purpose

    Lentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    tTR-KRAB-2A-rtTA3-2A-Luciferase
    iRFP670
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    EFS and pGK

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a(TIGIT)

    Plasmid

    #177902

    Purpose

    Bacterial expression of his10-tagged human TIGIT Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    TIGIT (aa 163-244 only) (TIGIT Human)
    

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. p168

    Plasmid

    #62618

    Purpose

    ePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLAR

    Depositor

    Mattheos Koffas

    Article

    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

    Inserts

    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    

    Use

    Synthetic Biology

    Tags

    GBD, PDZ, and SH3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFUGW-RapACR-EYFP

    Plasmid

    #111075

    Purpose

    Fast anion channelrhodopsin (current half-decay time 3-10 ms depending on voltage and light intensity) for time-resolved inhibition of neuronal spiking

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin RapACR
    

    Use

    Lentiviral

    Tags

    EYFP

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMM0641

    Plasmid

    #129006

    Purpose

    Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    

    Expression

    Yeast

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCFJ1208

    Plasmid

    #44490

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, unc-119]
    

    Use

    Gateway expression clone

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFUGW-RapACR_T111C-EYFP

    Plasmid

    #119195

    Purpose

    Fast anion channelrhodopsin mutant RapACR_T111C for time-resolved inhibition of neuronal spiking

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin RapACR fast mutant T111C
    

    Use

    Lentiviral

    Tags

    EYFP

    Mutation

    changed threonine 111 to cysteine

    Promoter

    Ubiquitin

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. All-in-one split-ADAR2

    Plasmid

    #170136

    Purpose

    AAV vector carrying ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. K401-iLid

    Plasmid

    #122484

    Purpose

    Component of optically-induced motor dimerization system

    Depositor

    Matt Thomson

    Article

    Ross et al Nature. 2019 Aug;572(7768):224-229. doi:

    Insert

    Kinesin 1-401 iLid His tag
    

    Tags

    His6 and iLid

    Expression

    Bacterial

    Mutation

    Kinesin amino acids 1-401

    Promoter

    T7

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. K20‐scFv‐pSMBP2

    Plasmid

    #159940

    Purpose

    To generate baculovirus for insect cell expression of anti beta-1 integrin scFv K20

    Depositor

    Sandra Schmid

    Article

    Lakoduk et al Traffic. 2020 Sep;21(9):590-602. doi: 10

    Insert

    anti beta-1 integrin scFv
    

    Tags

    6xHis tag, FLAG tag, MBP fusion, Sortase A recogn…

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3.1(-) + SLNCR1

    Plasmid

    #86816

    Purpose

    mammalian expression of lncRNA LINC00673/SLNCR1

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    LINC00673 (LINC00673 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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