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pPTK010-3a-Glucoamylase-αMFΔ

Plasmid

#84969

Purpose

Type 3a, Glucoamylase followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Glucoamylase followed by αMFΔ secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-NS

Plasmid

#75341

Purpose

Multisite gateway entry clone for adding N-terminal fusions of EGFP

Depositor

Rob Parton

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

EGFP

Expression

Mammalian

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pPTK020-7-attB

Plasmid

#84989

Purpose

Type 7, BxbI recognition site

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

BxbI recognition site

Use

Synthetic Biology

Expression

Yeast

Mutation

BsaI site removed (1711 t to a)

Promoter

Non

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK006-3a-αMF no EAEA

Plasmid

#84965

Purpose

Type 3a, α-mating factor no EAEA - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

α-mating factor no EAEA secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPath-pR-integrase

Plasmid

#200000

Purpose

Entry vector for biosynthetic pathway. pR-phiC31 integrase

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Pathways

Expression

Bacterial

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGE010-gR082

Plasmid

#188553

Purpose

Binary vector for CRISPR/Cas9 targeted to MpIGPD in Marchantia polymorpha (for Agrobacterium-mediated genetic transformation)

Depositor

Yutaka Kodama

Article

Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

Insert

gR082

Use

CRISPR; Entry vector

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pME-TagRFPt-NS

Plasmid

#75343

Purpose

Multisite gateway entry clone for adding N-terminal fusions of TagRFPt

Depositor

Rob Parton

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

TagRFPt

Expression

Mammalian

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Pink

Plasmid

#160445

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Pink chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Pink

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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C122-E53: mClover3

Plasmid

#162898

Purpose

Standard Gateway entry clone encoding mClover fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Clover3 fluorescent protein

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Orange

Plasmid

#160444

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Orange chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Orange

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCBLgRNA

Plasmid

#199726

Purpose

Gateway entry vector for single gRNA under OsU3 promoter.

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

sgRNA scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPTK018-3b-RFP_Sec

Plasmid

#84977

Purpose

Type 3b, Red fluorescent protein

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Red fluorescent protein

Use

Synthetic Biology

Expression

Yeast

Mutation

BsaI site removed (1677 c to g and 1794 a to g)

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pECNUS_P2

Plasmid

#194344

Purpose

sgRNA position entry clone 2

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Expression

Plant

Promoter

Ath U6 promoter

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pECNUS_P1

Plasmid

#194343

Purpose

sgRNA position entry clone 1

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Expression

Plant

Promoter

Ath U6 promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pECNUS_P3

Plasmid

#194345

Purpose

sgRNA position entry clone 3

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Expression

Plant

Promoter

Ath U6 promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pECNUS_P4

Plasmid

#194346

Purpose

sgRNA position entry clone 4

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Expression

Plant

Promoter

Ath U6 promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pECNUS_P5

Plasmid

#194347

Purpose

sgRNA position entry clone 5

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Expression

Plant

Promoter

Ath U6 promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pECNUS_P6

Plasmid

#194348

Purpose

sgRNA position entry clone 6

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Expression

Plant

Promoter

Ath U6 promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pECNUS_P7

Plasmid

#194349

Purpose

sgRNA position entry clone 7

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Expression

Plant

Promoter

Ath U6 promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pPTK001-2-pAOX1

Plasmid

#84960

Purpose

Type 2, pAOX1, Alcohol oxidase 1 promoter

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Alcohol oxidase 1 promoter

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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