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pAS

Plasmid

#64746

Purpose

Yeast expression plasmid: ACC1 (ser1157ala)

Depositor

Jens Nielsen

Article

Shi et al MBio. 2014 May 6;5(3):e01130-14. doi: 10

Insert

ACC1 (ser1157ala)

Expression

Yeast

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pYFP.LIC.DHFR

Plasmid

#83114

Purpose

Plasmid contains LIC cassette upstream of an in-frame copy of YFP and DHFR-TS selectable marker cassette for endogenous tagging on the gene locus.

Depositor

Vernon Carruthers

Article

Huynh et al Eukaryot Cell. 2009 Apr;8(4):530-9. doi:

Insert

YFP

Use

Toxoplasma gondii

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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PB-PGK-teD-Cit-hcvs-DHFR

Plasmid

#116050

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

teD-Cit-hcvs-DHFR

Expression

Mammalian

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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PB-PGK-tvD-Cit-tevs-DHFR

Plasmid

#116053

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

tvD-Cit-tevs-DHFR

Expression

Mammalian

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pJW1506

Plasmid

#62360

Purpose

RPL16b HA-TEV-AVI pop-in/pop-out plasmid

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

RPL16B

Use

Yeast targeting

Tags

HA-TEV-AVI

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pQHD

Plasmid

#48102

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of HA-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

HA tag

Expression

Bacterial

Promoter

PQ5

Available Since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pQFD

Plasmid

#48101

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of 3xFLAG-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

3xFLAG

Expression

Bacterial

Promoter

PQ5

Available Since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pT3TS-TAL-apobb.1-PR2-RA

Plasmid

#128696

Purpose

Right arm of TALEN pair 2 for cleavage of apobb.1 STOP locus

Depositor

Steven Farber

Article

Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi:

Insert

TALEN

Use

TALEN

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pT3TS-TAL-apobb.1-PR2-LA

Plasmid

#128695

Purpose

Left arm of TALEN pair 2 for cleavage of apobb.1 STOP locus

Depositor

Steven Farber

Article

Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi:

Insert

TALEN

Use

TALEN

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pWT062d

Plasmid

#107911

Purpose

W2m.2-4 plasmid. Contains H1-TetR-sgRNA B and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

H1-TetR-sgRNA B

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pWT062e

Plasmid

#107910

Purpose

W2m.2-3 plasmid. Contains U6-LacI-sgRNA A and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

U6-LacI-sgRNA A

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry.LIC.HXGPRT

Plasmid

#83364

Purpose

Plasmid contains LIC cassette upstream of an in-frame copy of mCherry and HXGPRT selectable marker cassette for endogenous tagging on the gene locus

Depositor

Vernon Carruthers

Article

Huynh et al Eukaryot Cell. 2009 Apr;8(4):530-9. doi:

Insert

mCherry

Use

Toxoplasma gondii

Tags

mCherry

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pQYD

Plasmid

#48104

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of SYFP2-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

SYFP2

Expression

Bacterial

Promoter

PQ5

Available Since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pET28b-Ava3856

Plasmid

#45742

Depositor

Emily Balskus, Christopher T Walsh

Article

Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

Insert

Ava3856 (Ava_3856 Anabaena variabilis)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
pUC19 gene 60

Plasmid

#49090

Purpose

Template for generating full length gene 60 mRNA by in vitro transcription with T7 RNA pol

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Promoter

T7

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pKEW270-pTet-empty

Plasmid

#115669

Purpose

Vector for anti-CRISPR expression with pTet

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

pTet

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pMOD3-mWasabi-Sh_ble

Plasmid

#120332

Purpose

Bacterial mWasabi promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mWasabi

Expression

Bacterial

Promoter

(None)

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pWT060f

Plasmid

#107907

Purpose

W2m.1-2 plasmid. Contains U6-sgRNA D (CCR5) and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

U6-sgRNA D (CCR5)

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pWT007f

Plasmid

#107891

Purpose

W1.0.3 plasmid. Contains PTetO-sd2U-Cas9, PLac-sgRNA1 and is part of CAMERA 1.0.3

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-sd2U-Cas9, PLac-sgRNA1

Expression

Bacterial

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pexA2_ring

Plasmid

#89672

Purpose

Plasmid for the construction of DNA-protein hybrid nanostructures. Template for the ring shown in figure 3C of associated article.

Depositor

Hendrik Dietz

Article

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

ring

Use

Unspecified

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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