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pLKO_HOXB13_#2

Plasmid

#70094

Purpose

Expression of shRNA to human HOXB13, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

HOXB13 (HOXB13 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
EF1a_HOXA10_P2A_Hygro_Barcode

Plasmid

#120452

Purpose

Barcoded lentiviral vector to express HOXA10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HOXA10 (HOXA10 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-KCNQ5__ALK

Plasmid

#116825

Purpose

Lentiviral expression of KCNQ5__ALK fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

KCNQ5__ALK

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe puro-HA-PIK3CA(H1047R;F486S;del)

Plasmid

#33255

Depositor

Thomas Roberts

Article

Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

Hemagglutinin (HA)

Expression

Mammalian

Mutation

changed histidine 1047 to arginine and phenylalan…

Promoter

SV40

Available Since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only
DDX11

Plasmid

#155502

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX11 (DDX11 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-HTATSF1__BRS3

Plasmid

#116822

Purpose

Lentiviral expression of HTATSF1__BRS3 fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

HTATSF1__BRS3

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSLIK JunD-HA neo

Plasmid

#58515

Purpose

Lentiviral expression vector for an inducible HA-tagged mouse JunD

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

JunD (Jund Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only
pMT[Hygro]_GBP-3xFlag-DEST

Plasmid

#157813

Purpose

A gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selection

Depositor

Zoltan Lipinszki

Article

Torras-Llort et al Commun Biol. 2020 Aug 19;3(1):454. doi:

Insert

GFP-binding protein

Use

For drosophila cells

Tags

3xFlag

Expression

Insect

Promoter

metallothionein

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF9_P2A_Hygro_Barcode

Plasmid

#120495

Purpose

Barcoded lentiviral vector to express KLF9 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF9 (KLF9 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAcBac1-Ma-sfGFP WT

Plasmid

#197567

Purpose

Expression of sfGFP WT with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP WT
M. alvus Pyl-tRNA (6) (4x copies)

Tags

V5-His6

Expression

Mammalian

Promoter

CMV and U6/H1

Available Since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX Bmx-SH2

Plasmid

#46409

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Bmx (BMX Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 286-400

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pQE-80L 2xHis-LRRK2 Armadillo 1-552

Plasmid

#186017

Purpose

2xHis-LRRK2 Armadillo 1-552

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

2xHis-LRRK2 Armadillo 1-552 (LRRK2 Human)

Expression

Bacterial

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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EF1a_ASCL3_P2A_Hygro_Barcode

Plasmid

#120428

Purpose

Barcoded lentiviral vector to express ASCL3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ASCL3 (ASCL3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_HOXA11_P2A_Hygro_Barcode

Plasmid

#120453

Purpose

Barcoded lentiviral vector to express HOXA11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HOXA11 (HOXA11 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBacMam2-DiEx-LIC-C entry vector

Plasmid

#111752

Purpose

Intermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with C-term FLAG tag. Baculovirus transfer vector for insect and mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Type

Empty backbone

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pSUMO CPTP

Plasmid

#170735

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

Insert

CPTP (CPTP Human)

Tags

SUMO

Expression

Bacterial

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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DCP2

Plasmid

#155499

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DCP2 (DCP2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF11_P2A_Hygro_Barcode

Plasmid

#120497

Purpose

Barcoded lentiviral vector to express KLF11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF11 (KLF11 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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MKV937/nlp-1p::FlincG3

Plasmid

#140508

Purpose

This construct contains cGMP biosensor driven by nlp-1 promoter for expression in PHB sensory neuron in pSMdelta backbone.

Depositor

Miri VanHoven

Article

Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

Insert

FlincG3

Expression

Worm

Promoter

nlp-1

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAC4 Frk-SH2

Plasmid

#46428

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Frk (FRK Human)

Tags

AviTag, GST, and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 108-213

Promoter

Trc

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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