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pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA

Plasmid

#133344

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets cpaA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_cpaA

Use

CRISPR

Promoter

constitutive

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-PGK-SNAP-MDMUT-SOX2

Plasmid

#115688

Purpose

Constitutive expression of a SOX2 protein tagged with SNAP-tag and a non-functional mitotic degron (MDMUT) for control

Depositor

David Suter

Article

Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

Insert

SNAP-MDMUT-SOX2

Use

Lentiviral

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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Wnt2B2 Non-STOP

Plasmid

#35889

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt2B2 (WNT2B Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M1WT

Plasmid

#89327

Purpose

Inducible expression of WT M1 spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted

Promoter

Tet-responsive P tight

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M87 WT

Plasmid

#89328

Purpose

Inducible expression of WT M87 spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-460 bp deleted

Promoter

Tet-responsive P tight

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pSG 413

Plasmid

#63859

Purpose

Detect translation initiation from CUG start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

CUG start codon

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
Gal4pBS4-Pleum

Plasmid

#60329

Purpose

Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Feb. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMpGWB223

Plasmid

#68614

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Triple repeats of Citrine (3xCitrine)

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB226

Plasmid

#68617

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pMpGWB209

Plasmid

#68600

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

3xFLAG

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pTJK517

Plasmid

#121474

Purpose

CEN TRP1 CNA1

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNA1

Use

Yeast cen vector

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-FOXD4

Plasmid

#153119

Purpose

Expression of Flag tagged human FOXD4 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXD4 (FOXD4 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pMpGWB429

Plasmid

#68694

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

tdTomato

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pFbx15(-1120/+114)-Luc

Plasmid

#13411

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pO-macs-ISH

Plasmid

#15856

Depositor

Peter Mombaerts

Article

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

Olfactory specific medium-chain acyl CoA synthetase (Acsm4 Mouse)

Use

Cloning vector

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pHW-CIBN-MP-HRW-CRY2olig-VHH(GFP)

Plasmid

#126026

Purpose

express RFP-tagged LARIAT variant for optogenetic clustering

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CIBN-MP; CRY2 PHRolig-VHH(GFP)

Tags

RFP

Expression

Insect

Mutation

CRY2 E490G

Promoter

Hsp70

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSO7

Plasmid

#106365

Purpose

To insert a single copy of a construct at a site on Chr I, corresponding to the Shigella flexneri protein OspF (a MAP kinase inhibitor) expressed specifically in the epidermis.

Depositor

Jonathan Ewbank

Article

Lee et al Virulence. 2018 Dec 31;9(1):648-658. doi

Insert

ttTi4348_ SEC _pcol-19_OspF_3'unc54UTR

Expression

Worm

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pGCaMP5

Plasmid

#78705

Purpose

GCaMP5 under the Toxoplasma SAG1 5' UTR. No Toxoplasma selectable marker

Depositor

Sebastian Lourido

Article

Sidik et al J Biol Chem. 2016 Apr 29;291(18):9566-80

Insert

GCaMP5

Promoter

SAG1 5' UTR

Available Since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

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Wnt1 Non-STOP

Plasmid

#35887

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt1 (WNT1 Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pLL3.7 Mor2 shRNA

Plasmid

#59298

Purpose

3rd generation lentiviral vector expressing shRNA to mouse Opmr1

Depositor

Ulrike Heberlein

Article

Lasek et al Genes Brain Behav. 2007 Nov;6(8):728-35.

Insert

Mor2 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only