We narrowed to 18,127 results for: Mut

1. pBEL1727

   Plasmid

   #139918

   Purpose

   Expression plasmid for LetB mutant Y814Bpa

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetB(43-877; Y814Amber)-6xHis

   Promoter

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBEL1726

   Plasmid

   #139917

   Purpose

   Expression plasmid for LetB mutant W476pa

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetB(43-877; W476Amber)-6xHis

   Promoter

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBEL1725

   Plasmid

   #139916

   Purpose

   Expression plasmid for LetB mutant F468Bpa

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetB(43-877; F468Amber)-6xHis

   Promoter

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBEL1811

   Plasmid

   #139915

   Purpose

   Expression plasmid for LetB mutant L358N

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetB(43-877; L359N)-6xHis

   Promoter

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBEL1810

   Plasmid

   #139914

   Purpose

   Expression plasmid for LetB mutant L358N

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetB(43-877; L358N)-6xHis

   Promoter

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBEL1809

   Plasmid

   #139913

   Purpose

   Expression plasmid for LetB mutant L355N

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetB(43-877; L355N)-6xHis

   Promoter

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBEL1808

   Plasmid

   #139912

   Purpose

   Expression plasmid for LetB mutant V247N

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetB(43-877; V247N)-6xHis

   Promoter

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBEL1807

   Plasmid

   #139911

   Purpose

   Expression plasmid for LetB mutant L246N

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetB(43-877; L246N)-6xHis

   Promoter

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBEL1806

   Plasmid

   #139910

   Purpose

   Expression plasmid for LetB mutant L243N

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetB(43-877; L243N)-6xHis

   Promoter

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBEL1862

    Plasmid

    #139909

    Purpose

    Complementation plasmid for LetAB with LetB mutant L359N

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(L359N)

    Promoter

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBEL1861

    Plasmid

    #139908

    Purpose

    Complementation plasmid for LetAB with LetB mutant L358N

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(L358N)

    Promoter

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBEL1860

    Plasmid

    #139907

    Purpose

    Complementation plasmid for LetAB with LetB mutant L355N

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(L355N)

    Promoter

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBEL1857

    Plasmid

    #139904

    Purpose

    Complementation plasmid for LetAB with LetB mutant L243N

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(L243N)

    Promoter

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENTR-R2-pA-L4

    Plasmid

    #141062

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pExo84-CRU 303

    Plasmid

    #131165

    Purpose

    For C-terminal genomic integration of Cub-R-URA3, HIS3 complements the histidine auxotrophy of S. cerevisiae his mutant

    Depositor

    Nils Johnsson

    Article

    Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

    Insert

    ScExo84
    

    Use

    Tags

    CRU

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAW228

    Plasmid

    #113242

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader tAggtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    TTGGTAG->tAggtag

    Promoter

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAW227

    Plasmid

    #113241

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Atggtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    TTGGTAG->Atggtag

    Promoter

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. relAE335Q_NS1

    Plasmid

    #100917

    Purpose

    expresses E.coli relAE335Q from the NS1 site in the S. elongatus PCC7942 genome

    Depositor

    Erin O'Shea

    Article

    Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

    Insert

    RelA fragment from E.coli with point mutation E335Q
    

    Use

    Cyanobacteria cloning vector

    Tags

    FLAG

    Expression

    Mutation

    E 335 converted to Q

    Promoter

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUG-UBC26

    Plasmid

    #104691

    Purpose

    Cloning Module

    Depositor

    Marco Trujillo

    Article

    Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

    Insert

    AtUBC26 (AT1G53025 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BpiI/BsaI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDB325

    Plasmid

    #113669

    Purpose

    expresses Ulp1_R1 in Escherichia coli

    Depositor

    Christopher Bahl

    Article

    Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

    Insert

    Ulp1_R1
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    contains solubility enhancing mutations

    Promoter

    T7

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pre-Ubc4 BrG pUC19

    Plasmid

    #111322

    Purpose

    for transcription to Ubc4 pre-mRNA

    Depositor

    Christine Guthrie

    Article

    Abelson et al Methods Enzymol. 2010;472:31-40. doi: 10

    Insert

    UBC4 (UBC4 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    intron has branch-site A to G mutation

    Promoter

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAW217

    Plasmid

    #113239

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtaC mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->ggtaC

    Promoter

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAW215

    Plasmid

    #113237

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggAag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->ggAag

    Promoter

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAW214

    Plasmid

    #113236

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader gCtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->gCtag

    Promoter

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAW213

    Plasmid

    #113235

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Cgtag mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->Cgtag

    Promoter

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAW216

    Plasmid

    #113238

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtTg mutant

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GGTAG->ggtTg

    Promoter

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHBS1333 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 G368W W385G)

    Plasmid

    #107855

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G368W and W385G in splicing reporter

    Promoter

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCS2TAL3_DD_NvFP-7R_Ex2-LEFT

    Plasmid

    #90036

    Purpose

    TALEN-mediated mutagenesis of Exon 2 in NvFP-7R

    Depositor

    Matthew Gibson

    Article

    Ikmi et al Nat Commun. 2014 Nov 24;5:5486. doi: 10.

    Insert

    7R_Ex2-LEFT
    

    Use

    TALEN

    Tags

    3x Flag and Fok1-DD domain

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCS2TAL3_RR_NvFP-7R_Ex2-RIGHT

    Plasmid

    #90037

    Purpose

    TALEN-mediated mutagenesis of Exon 2 in NvFP-7R

    Depositor

    Matthew Gibson

    Article

    Ikmi et al Nat Commun. 2014 Nov 24;5:5486. doi: 10.

    Insert

    7R_Ex2-RIGHT
    

    Use

    TALEN

    Tags

    Fok1-RR domain and HA

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNR166

    Plasmid

    #79547

    Purpose

    Output plasmid: contains 5-state register that regulates expression of GFP. Doesn't behave as expected.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Insert

    GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMZ036

    Plasmid

    #66973

    Purpose

    Expresses zebrafish Chk1

    Depositor

    Michael Lampson

    Article

    Zhang et al Cell Cycle. 2014;13(24):3828-38. doi: 10

    Insert

    zebrafish Chk1
    

    Use

    Zebrafish mrna injection

    Tags

    Expression

    Mammalian

    Mutation

    Q341H mutation compared to GenBank reference sequ…

    Promoter

    CMV

    Available Since

    Jan. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. c7-87/199_HLL

    Plasmid

    #63122

    Purpose

    Mm-cpn cysteine double mutant for crosslinking

    Depositor

    Tanja Kortemme

    Article

    Hoersch et al Nat Nanotechnol. 2013 Dec;8(12):928-32.

    Insert

    Mm-cpn c7_87/199_HLL (NEWENTRY )
    

    Use

    Tags

    6x HIS-tag

    Expression

    Bacterial

    Mutation

    C140S-C237S-C286A-C359T-C393A-C470S-C484S-K87C-S1…

    Promoter

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. c7-132/202_HLL

    Plasmid

    #63123

    Purpose

    Mm-cpn double cysteine mutant for crosslinking

    Depositor

    Tanja Kortemme

    Article

    Hoersch et al Nat Nanotechnol. 2013 Dec;8(12):928-32.

    Insert

    Mm-cpn c7_132/202_HLL (NEWENTRY )
    

    Use

    Tags

    6x HIS-tag

    Expression

    Bacterial

    Mutation

    C140S-C237S-C286A-C359T-C393A-C470S-C484S-Q132C-S…

    Promoter

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFA6a-VN155(I153L)-TRP1

    Plasmid

    #53193

    Purpose

    chromosomal tagging with Venus

    Depositor

    John Pringle

    Article

    Onishi et al J Cell Biol. 2013 Jul 22;202(2):311-29.

    Insert

    Venus
    

    Use

    Yeast genomic targeting

    Tags

    TRP1

    Expression

    Mutation

    aa 1–155, I to L mutation at aa 153

    Promoter

    Available Since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET29b-ClbP-S95A

    Plasmid

    #48245

    Purpose

    Untagged, full-length protein with active site mutation Serine95 to Alanine for in vivo peptidase assays

    Depositor

    Emily Balskus

    Article

    Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

    Insert

    ClbP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed Serine 95 to Alanine

    Promoter

    T7

    Available Since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pICH71411

    Plasmid

    #50345

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    3'UTR, polyadenylation signal/terminator, RbcS3C (S. lycopersicum )
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pICH44233

    Plasmid

    #50288

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    5'UTR CMV2 (Cucumber Mosaic Virus)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pICH44179

    Plasmid

    #50290

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    5' UTR, RbcS2B (AT5g38420, A. thaliana)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pICH37326

    Plasmid

    #50307

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    signal peptide, NbCRT calreticulin (Nicotiana benthamiana)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pICH45180

    Plasmid

    #50260

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    promoter, RbcS3B (AT5g38410, A. thaliana)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge