We narrowed to 63,598 results for: cell

1. Lenti-sgRNA(MS2)-puro-barcode

   Plasmid

   #89493

   Purpose

   Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

   Depositor

   Gary Hon

   Article

   Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

   Insert

   sgRNA (with MS2 loop)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP Tensin2

   Plasmid

   #105298

   Purpose

   To study localization and function of Tensin2

   Depositor

   Kenneth Yamada, David Critchley

   Article

   Clark et al J Cell Biochem. 2010 Mar 1;109(4):808-17

   Insert

   Tensin2 (TNS2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   lacks N-terminal C1 domain

   Promoter

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR_PGK_SNIPR_ADAM17 site

   Plasmid

   #188349

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a ADAM17site, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_ADAM17site_Notch1-TMD_Notch1-JMD_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFH2.20_mCherry-MS2x4

   Plasmid

   #205535

   Purpose

   Express mCherry-MS2x4 (export tag consisting of 4 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. UAS:Zebrabow-V

   Plasmid

   #118218

   Purpose

   For broad or variegated gal4 inducible expression of Brainbow in zebrafish

   Depositor

   Alex Schier, Albert Pan

   Article

   Pan et al Development. 2013 Jul;140(13):2835-46. d

   Insert

   Brainbow 1.0 L
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAG-Otx2-IRES-GFPd2

   Plasmid

   #73996

   Purpose

   Plasmid expressing Otx2(isoform b)-IRES-GFPd2

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   Otx2(isoform b)-Ires-GFP (Otx2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pILGFP95

   Plasmid

   #83550

   Purpose

   Used to evaluate the expression output of PDA1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   PDA1 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PDA1

   Available Since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pCFP-LacI-NLS

   Plasmid

   #103840

   Purpose

   Flourescent marker for lacO arrays

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CFP-LacI-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   LacI: C19T (silent, L7L), T264C (silent, A88A), C…

   Promoter

   SV2

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV U6-sgRNA UbC-tdTomato

   Plasmid

   #106949

   Purpose

   sgRNA cloning cassette using BsmBI/Esp3I enzymes with tdTomato marker

   Depositor

   Albert Edge

   Article

   Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

   Insert

   sgRNA BsmBI/Esp3I cloning site
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKI-cjACTB-IRES-ERT2CreERT2-IRES-Hyg

    Plasmid

    #159927

    Purpose

    Gene targeting

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Res. 2021 Jan 8;51:102164. doi

    Insert

    ERT2CreERT2
    

    Use

    Marmoset targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. ELF4_pet28a

    Plasmid

    #131661

    Purpose

    Bacterial expression of ETS gene ELF4

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ELF4 (ELF4 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXPR_212

    Plasmid

    #133457

    Purpose

    U6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistance

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    control guide
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAFNF-ECFP-Actb

    Plasmid

    #169787

    Purpose

    An actin FRET sensor. Must be expressed with Venus-actin for FRET.

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Actb (Actb Mouse)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-N1- Ncad-GFP

    Plasmid

    #35676

    Depositor

    James Jontes

    Article

    Biswas et al J Cell Biol. 2010 Nov 29;191(5):1029-41.

    Insert

    Ncad (cdh2 Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. L1-P941L

    Plasmid

    #15498

    Depositor

    Vance Lemmon

    Article

    Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

    Insert

    L1-P941L (L1CAM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Proline 941 changed to Leucine

    Promoter

    Available Since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pFH2.11_Gag-MCP_ZF2-P2A-eGFP

    Plasmid

    #205526

    Purpose

    Express HIV Gag-MCP_ZF2-P2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Gag-MCP_ZF2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GgVcl 1-1066 FRET tail probe 1

    Plasmid

    #46276

    Depositor

    Susan Craig

    Article

    Chen et al J Cell Biol. 2005 May 9;169(3):459-70.

    Insert

    Vcl 1-1066 + EYFP (VCL Chicken)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    addition of 9 bp linker and EYFP at aa 883

    Promoter

    CMV

    Available Since

    July 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF.mHES1.Ubc.GFP

    Plasmid

    #17625

    Depositor

    Linzhao Cheng

    Article

    Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

    Insert

    HES1 (Hes1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pXPR_213

    Plasmid

    #133456

    Purpose

    H1 promoter expresses customizable Saur-guide; EF1a promoter expresses SpyoCas9 and 2A site provides blasticidin resistance

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    Control guide
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHR-FRB:Akt2

    Plasmid

    #72899

    Purpose

    Recruitable Akt2 (LocaTOR2)

    Depositor

    Ivan Yudushkin

    Article

    Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

    Insert

    Serine/threonine protein kinase Bβ (AKT2 Human)
    

    Use

    Lentiviral

    Tags

    FRB(T2098L)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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