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Spacer Switch Plasmid

Plasmid

#215346

Purpose

Facilitates the change in spacer sequence to Neisseria gonorrhoeae encoding the Nla CRISPRi system.

Depositor

Hank Seifert

Article

Geslewitz et al mBio. 2023 Dec 21:e0302523. doi: 10.1128

Insert

OpaD spacer; Chloramphenicol Resistance Cassette

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAC150-KEIMA-RAMP4

Plasmid

#201929

Purpose

PiggyBac Vector expressing KEIMA-RAMP4

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

RAMP4 (SERP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAcBac1-Mb-sfGFP 150TAG

Plasmid

#197570

Purpose

Expression of sfGFP 150TAG with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP 150TAG
Pyl-tRNA (4 copies)

Use

Tags

V5-His6

Expression

Mammalian

Mutation

N150TAG

Promoter

CMV and U6/H1

Available since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAC1-V16-Luc

Plasmid

#122034

Purpose

AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and Luciferase expression

Depositor

Liliane Tenenbaum

Article

Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

Insert

Luciferase

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAc-GFPC1-Sec61beta

Plasmid

#15108

Depositor

Tom Rapoport

Insert

Sec61beta (SEC61B Human)

Use

Tags

Ac GFP

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only

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pAC5.1-dCry-V5His

Plasmid

#136237

Purpose

Protein expression of D. melanogaster (Dm) cryptochrome with C-terminal V5 and His tags

Depositor

Aziz Sancar, Steven Reppert

Article

Zhu et al Curr Biol. 2005 Dec 6;15(23):R953-4. doi

Insert

DmCry

Use

Tags

His and V5

Expression

Insect

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAcBac1.tR4-AcdRS82

Plasmid

#174101

Purpose

Expression of orthogonal Mb tRNA and Acd82 tRNA synthetase for incorporation of acridone in eukaryotic cells.

Depositor

Ryan Mehl

Article

Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

Insert

M. barkeri tRNA synthetase for acridone82

Use

Tags

Expression

Mammalian

Mutation

N311S, C313G, V366A, W382T

Promoter

CMV

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAC-U63-QtgRNA2.1-BR

Plasmid

#170513

Purpose

gRNA cloning vector containing a BFP Co-CRISPR reporter for tissue-specific CRISPR in somatic tissues.

Depositor

Chun Han

Article

Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

Type

Empty backbone

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

U6:3

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAC-U63-gRNA2.1

Plasmid

#170512

Purpose

gRNA cloning vector containing a U6:3 promoter and a gRNA2.1 scaffold.

Depositor

Chun Han

Article

Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

Type

Empty backbone

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

U6:3

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAcGFP-stop-IVT

Plasmid

#205146

Purpose

IVT template of AcGFP stop codon between the ORF and MCS and polyA

Depositor

Igor Ulitsky

Insert

AcGFP1

Use

In-vitro transcription

Tags

Expression

Mutation

Promoter

T7

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pAcBac1-BacMam-4x-PyOtR

Plasmid

#217364

Purpose

Expresses four copies pyrrolysyl tRNA variant "PyOtR" with U6 promoter; can be packaged in bacmid

Depositor

Abhishek Chatterjee

Article

Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

Insert

4 copies of pyrrolysyl-tRNA mutant PyOtR

Use

Baculoviral

Tags

Expression

Insect and Mammalian

Mutation

Promoter

U6

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-SpaCas12f1-gRNA_MS13

Plasmid

#190877

Purpose

The plasmid of CRISPR/SpaCas12f1(Syntrophomonas palmitatica Cas12f1) for genome editing in human cells

Depositor

Quanjiang Ji

Article

Wang et al Cell Rep. 2022 Sep 27;40(13):111418. doi

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAcBac1-GFP(39TAG)

Plasmid

#217360

Purpose

Expression of reporter EGFP with a TAG stop codon at 39 aa position; can be packaged into bacmid

Depositor

Abhishek Chatterjee

Article

Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

Insert

Enhanced green fluorescent protein

Use

Baculoviral

Tags

Expression

Insect and Mammalian

Mutation

Y39TAG

Promoter

CMV

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAC93-pmax-dCas9VP160

Plasmid

#48225

Purpose

dCas9VP160 on pmax expression vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP160 activation domain

Use

CRISPR

Tags

HA Tag and VP160

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pAcGP67A - murine TfR

Plasmid

#12392

Depositor

Pamela Bjorkman

Insert

transferrin receptor (Tfrc Mouse)

Use

Tags

6X-His tag, Factor Xa cleavage site, and leader p…

Expression

Insect

Mutation

See comments section

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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GFP-Epac1 CAAX

Plasmid

#118312

Purpose

Expression of human Epac1

Depositor

Philip Stork

Article

Wang et al Mol Cell Biol. 2006 Mar;26(6):2130-45. d

Insert

Epac1 CAAX (RAPGEF3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

C-terminal 20aa of KiRas

Promoter

CMV

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAcGP67A-mMD2-proA

Plasmid

#187878

Purpose

baculovirus protein expression of mouse MD-2 (residues 19–160) fused to a protein A tag

Depositor

Bruce Beutler

Article

Wang et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

MD2 (Ly96 Mouse)

Use

Tags

Expression

Insect

Mutation

includes residues 19–160 of mouse MD-2

Promoter

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pSYN-PACE-001

Plasmid

#42503

Depositor

Robert Peters

Article

Peters et al J Thromb Haemost. 2012 Dec 4. doi: 10.11

Insert

soluble PACE (FURIN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

cmv

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-mCherry-RspACFAST

Plasmid

#216349

Purpose

expresses mCherry-RspACFAST fusion cytosolically

Depositor

Alison Tebo

Insert

mCherry-RspACFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAC-Kan-alphaGal4

Plasmid

#13421

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

RNAP alpha-Gal4

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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