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pFOSGE_COF2H07

Plasmid

#70908

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CG7179 (CG7179 Fly)

Use

Gateway entry vector

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2H09

Plasmid

#70924

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Mnn1 (Mnn1 Fly)

Use

Gateway entry vector

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-5'UTR-MAVS-ORF3,4(KaganE50)

Plasmid

#52007

Purpose

Expresses the human MAVS CDS and contains the 5'UTR sequence with a mutation in the start codons 61 and 67 nt downstream of the FL MAVS start codon

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

5utr-MAVS-ORF2,3mut

Expression

Mammalian

Mutation

mutated start codons for ORF3 and 4

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
7a sgRNA for EJ7-GFP and 4-μHOM reporters

Plasmid

#113620

Purpose

sgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reporters

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7a sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
7b sgRNA for EJ7-GFP reporter

Plasmid

#113624

Purpose

sgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporter

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7b sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
7i sgRNA for 4-μHOM reporter

Plasmid

#113626

Purpose

sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter 8 nts. upstream from the microhomology

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7i sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
pJL1-CRM197-4xDQNAT

Plasmid

#128395

Purpose

In vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Diphtheria toxin

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Mutation

Inactivating G52E mutation

Promoter

T7

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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7h sgRNA for 4-μHOM reporter

Plasmid

#113625

Purpose

sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter at the edge of the microhomology.

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7h sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
pCAGGS-I-SceI-Trex2

Plasmid

#44024

Depositor

Jeremy Stark

Article

Gunn et al J Biol Chem. 2011 Dec 9;286(49):42470-82

Insert

I-SceI fused to mTREX2

Tags

NLS-HA

Expression

Mammalian

Promoter

pCAGGS

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pSF-CjPglB-LpxE

Plasmid

#128389

Purpose

Expression of C. jejuni PglB and F. tularensis LpxE in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Inserts

PglB oligosaccharylktransferase
LpxE phosphatase

Tags

Flag tag

Expression

Bacterial

Promoter

Ptac and pBAD

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJL1-PD-4xDQNAT

Plasmid

#128391

Purpose

In vitro expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Protein D

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF4A02

Plasmid

#71021

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Ube3a (Ube3a Fly)

Use

Gateway entry vector

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pGL3_4xUAS_CP-candidate_luc+

Plasmid

#125154

Purpose

STAP-seq luciferase validation vector, which harbors an 4x UAS array upstream of the CP position (candidates are inserted at CP position) for the recruitement of GAL4-DBD tagged proteins

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

4 x upstream activating sequence (UAS)

Use

Luciferase

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCoofy62

Plasmid

#122014

Purpose

Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His6-Smt3Star (SumoStar*) and different C-tag options

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His6-Smt3Star and Multi3: depending on the LP2 pr…

Expression

Mammalian

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJL1-PorA-4xDQNAT

Plasmid

#128392

Purpose

In vitro expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

PorA

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJL1-TTc-4xDQNAT

Plasmid

#128393

Purpose

In vitro expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Tetanus toxin fragment C domain

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3H08

Plasmid

#70996

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Nap1 (Nap1 Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pSTAP-seq_fly-ctrl

Plasmid

#86380

Purpose

Vector to measure the basal activity of CP candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Type

Empty backbone

Use

Stap-seq screening vector

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFOSGE_DUM1A01

Plasmid

#71088

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

pnr (pnr Fly)

Use

Gateway entry vector

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only
pFOSGE_CUS1A07

Plasmid

#71083

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

grn (grn Fly)

Use

Gateway entry vector

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2A03

Plasmid

#70878

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Elp3 (Elp3 Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF4D06

Plasmid

#71048

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

mor (mor Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3E07

Plasmid

#70987

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

TfIIS (TfIIS Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pGFP-AUG-IRES-mCherry

Plasmid

#222109

Purpose

Start codon reporter (WT AUG)

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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300_pETcon_SARS2_FLip

Plasmid

#222230

Purpose

yeast surface display of the SARS-CoV-2 FLip variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

Insert

SARS-CoV-2 FLip RBD (S Budding Yeast, SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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297_pETcon_SARS2_Omicron-BA286

Plasmid

#222231

Purpose

yeast surface display of the SARS-CoV-2 BA.2.86 variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

Insert

SARS-CoV-2 BA.2.86 RBD (S Budding Yeast, SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
299_pETcon_SARS2_EG-5

Plasmid

#222232

Purpose

yeast surface display of the SARS-CoV-2 EG.5 variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

Insert

SARS-CoV-2 EG.5 RBD (S Budding Yeast, SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_fly_spike-in

Plasmid

#125151

Purpose

Vector to measure CP candidates (spike-in) expression by determining the abundance of transcripts originating from the candidate by a D.pse enhancer in fly cells

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

D. pseudoobscura peak #47 enhancer

Use

Stap-seq screening vector

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3_zfh1_CP-candidate_luc+

Plasmid

#86391

Purpose

Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates responsiveness to the upstream enhancer is reflected by luciferase activity

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Insert

D. melanogaster zfh1 enhancer

Use

Luciferase

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only
pFOSGE_DUM1B01

Plasmid

#71089

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Clk (Clk Fly)

Use

Gateway entry vector

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only