We narrowed to 1,806 results for: pDest
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Plasmid#210485PurposepDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…ExpressionYeastPromoterADH1Available SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only
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pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term
Plasmid#182925PurposeExpress FLAG epitope and APEX2-tagged fusion protein in mammalian cells.DepositorTypeEmpty backboneTagsAPEX2 and FLAGExpressionMammalianPromoterCMVAvailable SinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-cDNA5-FRT/TO-3*Flag-APEX2 C-term
Plasmid#182926PurposeExpress FLAG epitope and APEX2-tagged fusion protein in mammalian cells.DepositorTypeEmpty backboneTagsAPEX2 and FLAGExpressionMammalianPromoterCMVAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST 3xFlag-pcDNA5-FRT/T0-53BP1 28A
Plasmid#52505Purposemammalian expression vector of 53BP1 28A mutantDepositorInsert53BP1 28A (TP53BP1 Human)
TagsFlagExpressionMammalianMutationAll 28 SQ/TQ motifs in N-term of 53BP1 have been …Available SinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST-CMV mCherry-GFP-LC3B G120A
Plasmid#123235PurposeExpresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
TagsEGFP and mCherryExpressionMammalianMutationGlycine 120 to AlaninePromoterCMVAvailable SinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Myc-FBW7 WD40 (All deleted)
Plasmid#197455PurposeThe plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 All are deleted. Used for mammalian overexpression and detection by western blotting.DepositorInsertFBW7 (FBXW7 Human)
TagsMycExpressionMammalianMutationFBW& dimerization domain deleted.PromoterCMVAvailable SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-mCherry-LacR-BRCA1 1366-1437
Plasmid#71118Purposemammalian expession of mCherry-LacR-BRCA1 1366-1437DepositorInsertBRCA1 (BRCA1 Human)
TagsmCherryExpressionMammalianMutationcontains amino acids 1366-1437 of BRCA1PromoterCMV/TOAvailable SinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSEM264 - pDESTR4-R3 mosTI Pmlc-2_GFP target
Plasmid#159842PurposeSingle copy or array insertion by MosTI using split Pmlc-2 GFP selectionDepositorInsertpDESTR4-R3 mosTI Pmlc-2_GFP target
ExpressionWormMutationNot applicableAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-K298M-Y419F-Y530F
Plasmid#140321PurposeExpresses Flag-tagged human Src-K298M-Y419F-Y530F in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
Tags2X FlagExpressionMammalianMutationK298M-Y419F-Y530FPromoterCMVAvailable SinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
hu-TLR4 299 SNP flag pLenti 4/to/v5 pDEST
Plasmid#42650Purposedestination vector for lentivirus-mediated expression of human TLR4 299 SNP in mammalian cellsDepositorInsertTLR4 299 SNP (TLR4 Human)
UseLentiviralTagsFlagExpressionMammalianMutationD299GPromoterCMV/TOAvailable SinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDN0605 (pDEST-DHFR F[1,2] N-term, NatR)
Plasmid#210487PurposepDEST vector to tag gene of interest with DHFR F[1,2] on the N-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…ExpressionYeastPromoterADH1Available SinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-K298M-Y419F-Y530F
Plasmid#140320PurposeExpresses HA-tagged human Src-K298M-Y419F-Y530F in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
Tags2X HAExpressionMammalianMutationK298M-Y419F-Y530FPromoterCMVAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-FRT/T0-GFP-BRCA1 1364-1437
Plasmid#71119PurposeMammalian expression of GFP-BRCA1 1364-1437DepositorInsertBRCA1 (BRCA1 Human)
TagsGFPExpressionMammalianMutationcontains amino acids 1364-1437 of BRCA1PromoterCMV/TOAvailable SinceDec. 9, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST-CMV 3xFLAG-LC3B-G120A-GFP
Plasmid#123108PurposeExpresses 3xFLAG-LC3B-G120A-EGFP in mammalian cells, negative control for monitoring ATG4 activity.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
Tags3xFLAG and EGFPExpressionMammalianMutationGlycine 120 to AlaninePromoterCMVAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CR-QUAS:mNeonGreen-NES-P2A-mKate2-NLS
Plasmid#241912PurposeZebrafish transgenesis plasmid encoding cytoplasmic mNeonGreen-NES and mKate2-NLS separated by the P2A peptide, driven by the QUAS enhancer, and including a cmlc2:mKate2 cardiac transgenesis markerDepositorInsertQUAS enhancer driving mNeonGreen-NES and mKate2-NLS
UseZebrafish tol2 transgenesisPromoterQUASAvailable SinceOct. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_EWSR1/ZNF384 fusion protein LCD (1-149)
Plasmid#235000PurposeBacterial expression of N-terminally 6His tagged EWSR1/ZNF384DepositorInsertTags6xHis-TEVExpressionBacterialPromoterT7Available SinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_THRAP3_THSD7A fusion protein LCD (663-955)
Plasmid#235002PurposeBacterial expression of N-terminally 6His tagged THRAP3_THSD7A (THRAP3) fusion protein LCDDepositorTags6xHis-TEVExpressionBacterialPromoterT7Available SinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::venus-RfA
Plasmid#186408PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter Venus under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-CFP
Plasmid#186412PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter CFP under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-venus
Plasmid#186409PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion N-ter Venus-gene of interest under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R4-bpFOG-B5::B1-NLSLacZ-B2
Plasmid#186414PurposeAttR3/R4 destination vector for ascidian electroporation with AttB1/B2 recombined NLSLacZ under control of AttB4/B5-recombined pFOG ectodermal regulatory sequence.DepositorInsertlacZ (lacZ E. coli)
UseGateway destination vectorTagsNuclear localization signalPromoterBasal promoter of the FOG geneAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV GFP-LC3B Q116P G120
Plasmid#129290PurposeExpresses GFP-tagged deconjugation-resistant LC3B in mammalian cellsDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
TagsGFPExpressionMammalianMutationChanged Glutamine 116 to Proline. Deleted amino a…PromoterCMVAvailable SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST15.1-hRPN13 (285-407) (Q337A/Q338A)
Plasmid#61935PurposeExpression of hRPN13 (285-407) (Q337A/Q338A) in E.coliDepositorInserthRPN13 (285-407) (ADRM1 Human)
TagsGSTExpressionBacterialMutationConstruct starts at residue 285, residues Q337 an…PromoterT7Available SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Tol2(Chien)-Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA
Plasmid#105643PurposeCo-expression of tdTomato and HyPer in differentiated keratinocytes of the periderm layer and ealy on also in the basal layer of the epidermisDepositorInsertKrt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA
ExpressionMammalianAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDest-Tol2 (Chien)-Krt4:Gal4VP16:tdTomato:5xUAS_MCS
Plasmid#105649PurposetdTomato expression in differentiated keratinocytes of the periderm layer and during early larval stages also in the basal layer of the epidermisDepositorInsertKrt4:Gal4VP16:tdTomato:5xUAS_MCS
ExpressionMammalianAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
hu-TLR4 299/399 dual snp flag pDEST40
Plasmid#42648Purposedestination vector for expression of human TLR4 299/399 SNP in mammalian cellsDepositorInsertTLR4 299/399 SNP (TLR4 Human)
TagsFlagExpressionMammalianMutationD299G and T399IPromoterCMVAvailable SinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CR-QUASM1:GCaMP7s-NLS-P2A-mKate2-NLS
Plasmid#241915PurposeZebrafish transgenesis plasmid encoding GCaMP7s-NLS and mKate2-NES separated by the P2A peptide, driven by the QUASM1 enhancer, and including a cmlc2:mKate2 cardiac transgenesis markerDepositorInsertQUASM1 enhancer driving GCaMP7s-NLS and mKate2-NLS
UseZebrafish tol2 transgenesisPromoterQUASM1Available SinceOct. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218)
Plasmid#229819PurposeA gateway compatible Tol2 destination vector containing the fli1a promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.DepositorTypeEmpty backboneUseTol2 destination vectorAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
Plasmid#159838PurposeThree-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arraysDepositorInsertpDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
ExpressionWormMutationNot applicableAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDest17_E3 ubiquitin-protein ligase (Topors) LCD (379-1045)
Plasmid#235005PurposeBacterial expression of N-terminally 6His tagged E3 ubiquitin-protein ligase (Topors) LCDDepositorInsertE3 ubiquitin-protein ligase (Topors) (TOPORS Human)
Tags6xHis-TEVExpressionBacterialPromoterT7Available SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only