We narrowed to 7,239 results for: ALP

1. pDGB3_alpha1-pAtUBQ10:HA-LRR-NELC492A-mcherry-Tnos

   Plasmid

   #211843

   Purpose

   Plant transient expression cassette for the E3-DART ligase under a UBQ10 promoter.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   LRR-NEL
   

   Use

   Synthetic Biology

   Tags

   HA and mCherry

   Expression

   Plant

   Mutation

   C492A

   Promoter

   UBIQUITIN10

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLVX-hSyn-Galpha-i3-IRESWT-Gbetagamma ONE-GO

   Plasmid

   #204343

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   hSyn Galpha-i3/Gbetagamma ONE-GO
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGB3_alpha1-AtUBQ10:HR1bR181/185A-GFP-T35S

   Plasmid

   #211839

   Purpose

   Plant transient expression cassette for GFP fused to the mutant HR1b degron sequence under UBQ10 promoter.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   HR1b
   

   Use

   Synthetic Biology

   Tags

   GFP

   Expression

   Plant

   Mutation

   R181A R185A

   Promoter

   UBIQUITIN10

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Stat1 alpha Y701F Flag pRc/CMV

   Plasmid

   #8702

   Depositor

   Jim Darnell

   Article

   Wen et al Cell 1995 Jul 28;82(2):241-50.

   Insert

   STAT1 alpha Y701F (STAT1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Y701F

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pAAV MP-135 mRFP-3MREs (AAV-alpha)

   Plasmid

   #206929

   Purpose

   Alpha cell-specific gene expression from AAV in human pancreatic islets

   Depositor

   Markus Grompe

   Insert

   mRFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MP-135

   Available since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

   Plasmid

   #128749

   Purpose

   Expresses control gRNA

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   Control sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PBS/pU6- HIF1 alpha RNAi plasmid 1

   Plasmid

   #21103

   Depositor

   Connie Cepko

   Article

   Chen et al Science. 2009 Jan 9. 323(5911):256-9.

   Insert

   RNAi against hypoxia inducible factor 1alpha
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Lenti-EF1alpha-VP64-dCas9-VP64-ZsG

   Plasmid

   #192667

   Purpose

   3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A ZsGreen1

   Depositor

   Jeffrey Miner

   Insert

   VP64-dCas9-VP64, ZsGreen1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Danio alpha A-crystallin 1kb promoter/AcGFP

   Plasmid

   #98096

   Purpose

   aA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

   Depositor

   Mason Posner

   Article

   Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

   Insert

   Alpha A-crystallin promoter 1 kb fragment, Danio rerio (cryaa Zebrafish)
   

   Use

   Gfp expressing

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Danio alpha A-crystallin 1 kb fragment (-1028/-1)

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLVX-hSyn-Galpha-s-IRESWT-Gbetagamma ONE-GO

    Plasmid

    #204342

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    hSyn Galpha-s/Gbetagamma ONE-GO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGB3 alpha1 P35S:PhiC31 integrase-RDF:T35S (GB2893)

    Plasmid

    #160633

    Purpose

    TU for the constitutive expression of the PhiC31 integrase, C-terminally fused with its reversion factor (RDF)

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31-RDF
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAGGS-mNG-eDHFR(69K6)-G-alpha-q(L254A)

    Plasmid

    #178851

    Purpose

    Mammalian expression of Gαq(L254A) fused to mNeonGreen-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    mNeonGreen-eDHFR(69K6)-Gαq(L254A) (GNAQ Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Gαq: C9S/C10S/Q209L/L254A

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-EF1alpha-scFv-p65-HSF1-Blast

    Plasmid

    #192652

    Purpose

    3rd generation lenti vector encoding scFV-p65-HSF1 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    scFv-p65-HSF1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-FKBP-C-mSOG-J-alpha-IRES-mCherry

    Plasmid

    #136603

    Purpose

    Encodes FKBP tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).

    Depositor

    John Ngo

    Article

    Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

    Insert

    FKBP-C-mSOG-J-alpha
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PBS/pU6-HIF1 alpha RNAi plasmid 2

    Plasmid

    #21104

    Depositor

    Connie Cepko

    Article

    Chen et al Science. 2009 Jan 9. 323(5911):256-9.

    Insert

    RNAi against hypoxia inducible factor 1alpha
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPICZ-pOst1-pro-alphaf(MUT1)-E2-Crimson

    Plasmid

    #117662

    Purpose

    Test intracellular localisation of E2-Crimson and study the secretion efficiency

    Depositor

    Benjamin Glick, Pau Ferrer

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pOst1-pro-af(MUT1)-E2-Crimson
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    This plasmid encodes the fluorescent protein E2-C…

    Promoter

    pAOX1

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. V308 pDNR MCS lacZ alpha - Donor

    Plasmid

    #11691

    Depositor

    Tony Pawson

    Article

    Colwill et al BMC Biotechnol. 2006 . 6():13.

    Type

    Empty backbone

    Use

    Cre/Lox; Donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pLenti-EF1alpha-IRES-EGFP-Flag-hRPGRIP1L

    Plasmid

    #122991

    Purpose

    Expresses Flag-tagged hRPGRIP1L in mammalian cells

    Depositor

    Ken-Ichi Takemaru, FENG-QIAN Li

    Insert

    RPGRIP1L (RPGRIP1L Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p(HA)HIF1alpha(401delta603) (L795V, C800S, L818S, L822V)

    Plasmid

    #52216

    Purpose

    mammalian expression of HA tagged HIF1alpha with a deletion of the N-terminal activation domain and LCLL mutation

    Depositor

    Eric Huang

    Article

    Koshiji et al EMBO J. 2004 May 5;23(9):1949-56. Epub 2

    Insert

    HIF1alpha (401delta603) LCLL (HIF1A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa 401-603 deleted, removes the oxygen-dependent …

    Promoter

    CMV

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pCMV5-Flag-EPLIN alpha delta LIM

    Plasmid

    #40933

    Depositor

    Scott Maul, Elizabeth Luna

    Article

    Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

    Insert

    EPLIN alpha (LIMA1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    LIM domain deleted- amino acids 221-295

    Promoter

    Available since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

21. pU6-mir144 EF1Alpha-puro-T2A-BFP

    Plasmid

    #164791

    Purpose

    Express miR-144 under the U6 promoter in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-144 (MIR144 Human)
    puro-T2A-BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha and U6

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV5-Flag-EPLIN alpha delta C-term

    Plasmid

    #40930

    Depositor

    Scott Maul, Elizabeth Luna

    Article

    Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

    Insert

    EPLIN alpha (LIMA1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 296-600

    Promoter

    Available since

    Dec. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

    Plasmid

    #164800

    Purpose

    For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    ERH CRISPRi gRNA1
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha and U6

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

    Plasmid

    #164801

    Purpose

    For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    ERH CRISPRi gRNA2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha and U6

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pU6-mir451 EF1Alpha-puro-T2A-BFP

    Plasmid

    #164792

    Purpose

    Express miR-451 under the U6 promoter in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    microRNA 451a (MIR451A Human)
    puro-T2A-BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha and U6

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Venus Cam KII alpha mutant T286D

    Plasmid

    #29429

    Depositor

    Steven Vogel

    Article

    Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

    Insert

    Venus CAM KII Alpha
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    T286D mutation in CamKIIa sequence

    Promoter

    Available since

    July 5, 2011

    Availability

    Academic Institutions and Nonprofits only

27. pALPS_3'LTR GFP@gag start SFFV-

    Plasmid

    #101337

    Purpose

    Repaired U3 allows LTR-based transcription by the provirus with GFP as a marker for expression. WPRE was deleted.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Venus Cam KII alpha mutant T286A

    Plasmid

    #29430

    Depositor

    Steven Vogel

    Article

    Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

    Insert

    Venus CAM KII Alpha
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    T286A mutation in CamKIIa sequence

    Promoter

    Available since

    June 3, 2011

    Availability

    Academic Institutions and Nonprofits only

29. pENTR/D-Topo genomic A. thaliana IMPORTIN ALPHA 1

    Plasmid

    #175814

    Purpose

    pENTR plasmid with genomic sequence of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 1 (AT3G06720) without stop codon for C-terminal epitope tags

    Depositor

    Mark Banfield, Lennart Wirthmueller

    Article

    Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1

    Insert

    IMPORTIN ALPHA ISOFORM 1 (IMPA-1 Mustard Weed)
    

    Use

    Pentr plasmid for gateway cloning

    Tags

    none

    Expression

    Mutation

    Promoter

    none

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDGB3 alpha1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1656)

    Plasmid

    #160618

    Purpose

    Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).

    Depositor

    Diego Orzaez

    Insert

    Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos, 35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA Flag-RARalpha (T181A,S445A,S461A)

    Plasmid

    #35556

    Depositor

    Jonathan Kurie, Harish Srinivas

    Article

    Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

    Insert

    RARalpha (RARA Human)
    

    Use

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    T181A, S445A, S461A

    Promoter

    Available since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

32. pJM450 EF1alpha EBFP2-P2A-BlastR (TUPV1)

    Plasmid

    #139230

    Purpose

    mMoClo TUPV1, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBSSK p85 alpha (delta SH3, iSH2)

    Plasmid

    #13433

    Depositor

    Ronald Kahn

    Article

    Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

    Insert

    p85 alpha (delta SH3, iSH2) (PIK3R1 Human)
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Flag tags replacing both the SH3 and iSH2 domains.

    Promoter

    Available since

    Nov. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

34. Danio alpha Bb-crystallin 1kb promoter/AcGFP

    Plasmid

    #98098

    Purpose

    aBb-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

    Depositor

    Mason Posner

    Article

    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

    Insert

    Alpha Bb-crystallin promoter 1 kb fragment, Danio rerio (cryabb Zebrafish)
    

    Use

    Gfp expressing

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Danio aBb-crystallin 1 kb fragment (-1074/-1)

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Danio alpha Ba-crystallin 3kb promoter/AcGFP

    Plasmid

    #98097

    Purpose

    aBA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

    Depositor

    Mason Posner

    Article

    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

    Insert

    Alpha Ba-crystallin promoter 3 kb fragment, Danio rerio (cryaba Zebrafish)
    

    Use

    Gfp expressing

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Danio alpha Ba-crystallin 3 kb fragment (-3000/-1)

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSG5-FLAG-CaMKK alpha with CaMKKbeta RP domain

    Plasmid

    #33331

    Depositor

    Anthony Means

    Article

    Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

    Insert

    Calcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    CaMKKalpha RP domain (167-189) was replaced with …

    Promoter

    SV40

    Available since

    April 10, 2012

    Availability

    Academic Institutions and Nonprofits only

37. V677 pDNR MCS BE lacZ alpha - Donor

    Plasmid

    #11694

    Depositor

    Tony Pawson

    Article

    Colwill et al BMC Biotechnol. 2006 . 6():13.

    Type

    Empty backbone

    Use

    Cre/Lox; Donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pCMV5-Flag-EPLIN alpha delta domain 7

    Plasmid

    #40940

    Depositor

    Scott Maul, Elizabeth Luna

    Article

    Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

    Insert

    EPLIN alpha delta Domain 7 (LIMA1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 562-600

    Promoter

    Available since

    Dec. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

39. pCMV5-Flag-EPLIN alpha delta domain 3

    Plasmid

    #40937

    Depositor

    Scott Maul, Elizabeth Luna

    Article

    Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

    Insert

    EPLIN alpha delta Domain 3 (LIMA1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 106-148

    Promoter

    Available since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

40. pCMV5-Flag-EPLIN alpha delta N54

    Plasmid

    #40934

    Depositor

    Scott Maul, Elizabeth Luna

    Article

    Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

    Insert

    EPLIN (LIMA1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-54

    Promoter

    Available since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only