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We narrowed to 3,503 results for: cgas

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Showing: 1041 - 1080 of 3503 results

  3. AAV-KrasWT/sgKras/Cre

    Plasmid
    #99848
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template that serves as a control for HDR into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99848-plasmid-map-sequence-id-193786

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  4. AAV-KrasG12R/sgKras/Cre

    Plasmid
    #99852
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12R mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99852-plasmid-map-sequence-id-193774

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  6. AAV-KrasG12S/sgKras/Cre

    Plasmid
    #99853
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99853-plasmid-map-sequence-id-193755

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  7. AAV-KrasG13V/sgKras/Cre

    Plasmid
    #99860
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13V mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99860-plasmid-map-sequence-id-193770

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  9. AAV-KrasG13A/sgKras/Cre

    Plasmid
    #99855
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13A mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Available Since
    May 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    99855-plasmid-map-sequence-id-200262

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  10. hNTo2-qgRNA-pYJA5

    Plasmid
    #217782
    Purpose
    Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    217782-plasmid-map-sequence-id-466020

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  11. hNTa2-qgRNA-pYJA5

    Plasmid
    #217780
    Purpose
    Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    217780-plasmid-map-sequence-id-469426

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  13. gCH130 (crCD55-4_crB2M-1_crKIT-2_crCD81-1)

    Plasmid
    #217340
    Purpose
    crRNA array targeting CD81, B2M, KIT, CD55
    Depositor
    Luke Gilbert
    Article
    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    Use
    CRISPR and Lentiviral
    Promoter
    hU6
    Available Since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    217340-plasmid-map-sequence-id-426478

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  16. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118162
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available Since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118162-plasmid-map-sequence-id-240427

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  17. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide4

    Plasmid
    #118180
    Purpose
    CRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118180-plasmid-map-sequence-id-240371

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  19. pCGLH-luciferase

    Plasmid
    #194979
    Purpose
    pCGLH control plasmid
    Depositor
    Angelique Bordey
    Insert
    luciferase
    Expression
    Bacterial
    Available Since
    Feb. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    194979-plasmid-map-sequence-id-386135

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  20. pAAV Syn Barcode16

    Plasmid
    #226195
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    Syn Barcode16
    Use
    AAV
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226195-plasmid-map-sequence-id-447891

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  21. pAAV Syn Barcode19

    Plasmid
    #226196
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    Syn Barcode19
    Use
    AAV
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226196-plasmid-map-sequence-id-447886

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  22. pAAV Syn Barcode18

    Plasmid
    #226191
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    Syn Barcode18
    Use
    AAV
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226191-plasmid-map-sequence-id-447872

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  23. Lentiviral nontargeting shRNA GFP

    Plasmid
    #155285
    Purpose
    Lentiviral expression of nontargeting shRNA, GFP expression, based on Addgene 12247
    Depositor
    Martin Carroll
    Insert
    Nontargeting shRNA
    Expression
    Mammalian
    Available Since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155285-plasmid-map-sequence-id-311904

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  24. Banshee shTCF1 mCherry

    Plasmid
    #80154
    Purpose
    expresses shRNA targeting TCF1 and mCherry
    Depositor
    Ellen Rothenberg
    Insert
    TCF1
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    80154-plasmid-map-sequence-id-158534

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  25. lentiCRISPRv2_ZsG gRNA_4

    Plasmid
    #192688
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #4
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a, U6
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    192688-plasmid-map-sequence-id-379587

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  26. lentiCRISPRv2_ZsG gRNA_3

    Plasmid
    #192687
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #3
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a, U6
    Available Since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    192687-plasmid-map-sequence-id-379588

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  27. pTJK643

    Plasmid
    #138011
    Purpose
    SIX1 knockout
    Depositor
    Tami Kingsbury
    Insert
    SIX1 sgRNA1 (SIX1 Human)
    Use
    CRISPR and Lentiviral
    Mutation
    None
    Promoter
    U6
    Available Since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138011-plasmid-map-sequence-id-275015

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  28. PCRII-zSynA

    Plasmid
    #20121
    Depositor
    Aaron Gitler
    Insert
    zSynA (sncgb Zebrafish)
    Use
    In-situ
    Available Since
    Jan. 20, 2009
    Availability
    Academic Institutions and Nonprofits only

  29. Dox nontargeting shRNA GFP

    Plasmid
    #155290
    Purpose
    Lentivirus for doxycycline inducible expression of nontargeting shRNA, GFP marker, based on Addgene 11652
    Depositor
    Martin Carroll
    Insert
    Nontargeting shRNA
    Expression
    Mammalian
    Available Since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155290-plasmid-map-sequence-id-475763

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  30. Lentiviral nontargeting shRNA RFP i670

    Plasmid
    #155287
    Purpose
    Lentiviral expression of nontargeting shRNA, RFP i670 expression, based on Addgene 12247
    Depositor
    Martin Carroll
    Insert
    Nontargeting shRNA
    Expression
    Mammalian
    Available Since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155287-plasmid-map-sequence-id-310003

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  31. Dox nontargeting shRNA mKate

    Plasmid
    #155292
    Purpose
    Lentivirus for doxycycline inducible expression of nontargeting shRNA, mKate marker, based on Addgene 11652
    Depositor
    Martin Carroll
    Insert
    Nontargeting shRNA
    Expression
    Mammalian
    Available Since
    Sept. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    155292-plasmid-map-sequence-id-310436

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  32. pZIC3.1.0-gDNA

    Plasmid
    #176379
    Purpose
    Expresses spCas9 and gRNA sequence targeting ZIC3
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ZIC3 gRNA
    Use
    CRISPR
    Available Since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    176379-plasmid-map-sequence-id-347383

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  33. PCRII-zSynB

    Plasmid
    #20122
    Depositor
    Aaron Gitler
    Insert
    zSynB (sncga Zebrafish)
    Use
    In-situ
    Available Since
    Jan. 9, 2009
    Availability
    Academic Institutions and Nonprofits only

  34. BII-Sh-BnTW

    Plasmid
    #133365
    Purpose
    Piggybac vector for shRNA-mediated knockdown, with co-expression with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Tags
    Flag-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available Since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133365-plasmid-map-sequence-id-268571

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  35. HCP8

    Plasmid
    #166110
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Whi5
    Depositor
    Matthias Heinemann
    Insert
    Whi5-sg106 (WHI5 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166110-plasmid-map-sequence-id-330382

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  36. LT3GEPIR-NEK11shRNA1

    Plasmid
    #220558
    Purpose
    To inducibly knockdown NEK11 expression
    Depositor
    Sean Lee
    Insert
    NEK11 shRNA1 (NEK11 Human)
    Use
    Lentiviral
    Promoter
    TRE3G (TetOP) promoter
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220558-plasmid-map-sequence-id-437948

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  37. pENN.AAV.SV40-si-VAMP3

    Plasmid
    #187864
    Purpose
    a human siRNA-expressing plasmid targeting VAMP3
    Depositor
    Yan Zeng
    Insert
    si-VAMP3 (VAMP3 Human)
    Use
    AAV
    Available Since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    187864-plasmid-map-sequence-id-370195

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  38. pAAV.CMV.PI-si-VAMP3

    Plasmid
    #187860
    Purpose
    a human siRNA-expressing plasmid targeting VAMP3
    Depositor
    Yan Zeng
    Insert
    si-VAMP3 (VAMP3 Human)
    Use
    AAV
    Available Since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    187860-plasmid-map-sequence-id-370036

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  39. pCENPBD1.1.0-gDNA

    Plasmid
    #132460
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    CENPBD1 (CENPBD1 Human)
    Use
    CRISPR
    Available Since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    132460-plasmid-map-sequence-id-267023

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  40. pFG001

    Plasmid
    #62816
    Purpose
    Bacterial constitutive gRNA expression plasmid
    Depositor
    Sébastien Rodrigue
    Insert
    gRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    PN25
    Available Since
    Nov. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    62816-plasmid-map-sequence-id-123824

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Showing: 1041 - 1080 of 3503 results