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Showing: 1301 - 1350 of 17682 results

  5. pACYC Destination

    Plasmid
    #207675
    Purpose
    Trio “destination vectors” which harbor an operon for constitutive expression of the repressors for each inducible promoter (CymR, PhlF, and VanR for cumate, DAPG and vanillate, respectively).
    Depositor
    Ramon Gonzalez
    Article
    Lee et al Metab Eng. 2024 Mar;82:262-273. doi: 10.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    CymRC, PhlF, VanCC
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    207675-plasmid-map-sequence-id-421910

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  6. SUMO_MT3

    Plasmid
    #200303
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility
    Depositor
    Artur Krężel
    Article
    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.
    Insert
    metallothionein 3 (MT3 Human)
    Use
    Tags
    Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    200303-plasmid-map-sequence-id-394864

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  9. pJH4140

    Plasmid
    #191479
    Purpose
    Pacr-2s-loxP::EBFP::loxP::Chrimson::wCherry unc-54 3' UTR C.elegans A/B MN expression of Arch RFP
    Depositor
    Mei Zhen
    Article
    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e
    Insert
    Chrimson
    Use
    Tags
    wCherry
    Expression
    Worm
    Mutation
    Promoter
    Pacr-2s
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    191479-plasmid-map-sequence-id-377517

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  12. pJH3557

    Plasmid
    #191444
    Purpose
    Pttr-39-Chrimson::wCherry unc-54 3' UTR C.elegans D motor neurou expression of Chrimson RFP
    Depositor
    Mei Zhen
    Article
    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e
    Insert
    Chrimson
    Use
    Tags
    wCherry
    Expression
    Worm
    Mutation
    Promoter
    Pttr-39
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    191444-plasmid-map-sequence-id-377550

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  15. pJH2684

    Plasmid
    #192061
    Purpose
    Pacr-5-TeTx::wCherry unc-54 3' UTR C.elegans B-MN and other neurons expression of TeTx RFP
    Depositor
    Mei Zhen
    Article
    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e
    Insert
    Tetanus Toxin Light Chain
    Use
    Tags
    wCherry
    Expression
    Worm
    Mutation
    Promoter
    Pacr-5
    Available since
    Nov. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    192061-plasmid-map-sequence-id-375426

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  16. pJH4211

    Plasmid
    #191532
    Purpose
    Pacr-2s-LoxP::EBFP::LoxP::GtACR2::wCherry unc-54 3' UTR C.elegans A/B MN expression of GtACR2 RFP
    Depositor
    Mei Zhen
    Article
    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e
    Insert
    GtACR2
    Use
    Tags
    wCherry
    Expression
    Worm
    Mutation
    Promoter
    Pacr-2s
    Available since
    Nov. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    191532-plasmid-map-sequence-id-375273

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  17. pOP004

    Plasmid
    #185985
    Purpose
    Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly upstream of the intrinsic terminator
    Depositor
    Georgi Belogurov
    Article
    Huang et al Methods Enzymol. 2022;675:207-233. doi:
    Insert
    Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    185985-plasmid-map-sequence-id-378299

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  18. pOP005

    Plasmid
    #186030
    Purpose
    Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly downstream of the promoter
    Depositor
    Georgi Belogurov
    Article
    Huang et al Methods Enzymol. 2022;675:207-233. doi:
    Insert
    Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    186030-plasmid-map-sequence-id-371540

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  23. pSMART-Ala2

    Plasmid
    #71326
    Purpose
    NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression
    Depositor
    Michael Lynch
    Article
    Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.
    Insert
    NADPH Alanine Dehydrogenase (ald Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    D196A, L197R
    Promoter
    insulated waaHp from E. coli
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    71326-plasmid-map-sequence-id-132780

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  27. AlkB-D135G

    Plasmid
    #160434
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly
    Depositor
    Tao Pan
    Article
    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30
    Insert
    E. coli AlkB
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Glycine; deleted the…
    Promoter
    T7
    Available since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    160434-plasmid-map-sequence-id-318954

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  30. pTCW018

    Plasmid
    #83531
    Purpose
    Aromatic amino acid inducible expression of alpha pheromone, and pheromone inducible exression of an ARO7 RNA interference hairpin
    Depositor
    Claudia Vickers
    Article
    Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.
    Insert
    pARO9-mf_2-pFUS1J2-ARO7i-CYC1t
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pARO9/pFUS1J2
    Available since
    Oct. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    83531-plasmid-map-sequence-id-159463

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  31. pCas59

    Plasmid
    #82396
    Purpose
    sgRNA targeting YFP +787 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance
    Depositor
    Brian Pfleger
    Article
    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(
    Insert
    gRNA
    Use
    Tags
    Expression
    Mutation
    Promoter
    PEZ3
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82396-plasmid-map-sequence-id-156156

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  32. pCas57

    Plasmid
    #82395
    Purpose
    sgRNA targeting YFP +111 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance
    Depositor
    Brian Pfleger
    Article
    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(
    Insert
    gRNA
    Use
    Tags
    Expression
    Mutation
    Promoter
    PEZ3
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    82395-plasmid-map-sequence-id-161477

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  33. pCas56

    Plasmid
    #82394
    Purpose
    sgRNA targeting YFP +46 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance
    Depositor
    Brian Pfleger
    Article
    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(
    Insert
    gRNA
    Use
    Tags
    Expression
    Mutation
    Promoter
    PEZ3
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    82394-plasmid-map-sequence-id-162297

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  34. pCas55

    Plasmid
    #82393
    Purpose
    sgRNA targeting YFP +172 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance
    Depositor
    Brian Pfleger
    Article
    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(
    Insert
    gRNA
    Use
    Tags
    Expression
    Mutation
    Promoter
    PEZ3
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    82393-plasmid-map-sequence-id-162303

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  35. pCas52

    Plasmid
    #82392
    Purpose
    sgRNA targeting YFP +52 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance
    Depositor
    Brian Pfleger
    Article
    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(
    Insert
    gRNA
    Use
    Tags
    Expression
    Mutation
    Promoter
    PEZ3
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    82392-plasmid-map-sequence-id-162311

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  41. pEvol-MjaYRS

    Plasmid
    #153557
    Purpose
    Amber suppression for incorporating 3-aminotyrosine to proteins in E. coli
    Depositor
    Huiwang Ai
    Article
    Zhang et al Nat Chem Biol. 2020 Sep 14. pii: 10.1038
    Inserts
    MJaYRS (first copy)
    MJaYRS (second copy)
    amber suppression tRNA under proK promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    glnS and pBAD
    Available since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    153557-plasmid-map-sequence-id-306290

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  47. pC1-SypHer3s-IMS

    Plasmid
    #108120
    Purpose
    Mitochondrial ratiometric pH probe with enhanced brightness fused to Smac/DIABLO fragment. Probe is localized in mitochondrial intermembrane space. Mammalian cell expression.
    Depositor
    Vsevolod Belousov
    Article
    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.
    Insert
    SypHer3s-IMS
    Use
    Tags
    ratiometricYFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    108120-plasmid-map-sequence-id-262557

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Showing: 1301 - 1350 of 17682 results