We narrowed to 4,269 results for: Bre;

1. pAG415Gal_Gcn4 SATtoG KtoR

   Plasmid

   #232961

   Purpose

   Galactose iduced expression of Gcn4 ATtoG KtoR in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 SATtoG KtoR
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG415Gal_Gcn4 ILVtoED solvvol

   Plasmid

   #232958

   Purpose

   Galactose iduced expression of Gcn4 LVtoED solvvol in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoED solvvol
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG415Gal_Gcn4 SATtoG KtoRGFP

   Plasmid

   #233006

   Purpose

   Galactose iduced expression of Gcn4 SATtoG KtoRGFP in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 SATtoG KtoR
   

   Use

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDest17_Gcn4 ILVtoED solvvol

   Plasmid

   #231858

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED solvvol

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoED solvvol
   

   Use

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

   Promoter

   T7

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. SBC015898

   Plasmid

   #226285

   Purpose

   Expresses PhCFAT, ObEGS1, and ObEOMT1 from trc promoter. Biosynthesis of methylated phenylpropenes from monolignol precursors.

   Depositor

   Rainer Breitling

   Article

   Chua et al Metab Eng Commun. 2024 May 15;18:e00237.

   Inserts

   Coniferyl alcohol acyltransferase
   Eugenol synthase 1
   Eugenol O-methyltransferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-PML RING

   Plasmid

   #185631

   Purpose

   expression of GST-tagged PML RING domain in bacterial cells

   Depositor

   Irina Bezsonova

   Article

   Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

   Insert

   promyelocytic leukemia protein (PML Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-PML RB1

   Plasmid

   #185636

   Purpose

   expression of GST-tagged PML fragment 49-168 (RING + B-box1) in bacterial cells

   Depositor

   Irina Bezsonova

   Article

   Bregnard et al Biophys Chem. 2022 Aug;287:106827. doi:

   Insert

   promyelocytic leukemia protein (PML Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-NHERF1 3xSA

   Plasmid

   #195207

   Purpose

   Mammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphorylation-deficient mutant (S162/339/340A)

   Depositor

   David Bryant, Anthony Bretscher

   Article

   Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

   Insert

   NHERF1 (NHERF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S162/339/340A

   Promoter

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-NHERF1 3xSD

   Plasmid

   #195208

   Purpose

   Mammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphomimetic mutant (S162/339/340D)

   Depositor

   David Bryant, Anthony Bretscher

   Article

   Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

   Insert

   NHERF1 (NHERF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S162/339/340D

   Promoter

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-NHERF1 PDZ1/2*

    Plasmid

    #195211

    Purpose

    Mammalian expression vector for GFP tagged NHERF1. Dual PDZ1/2 GYGF>GYAA motif mutant

    Depositor

    David Bryant, Anthony Bretscher

    Article

    Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

    Insert

    NHERF1 (NHERF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Dual PDZ1/2 GYGF>GYAA motif mutant

    Promoter

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pINDUCER21 (ORF-EG)

    Plasmid

    #46948

    Purpose

    Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

    Depositor

    Thomas Westbrook, Stephen Elledge

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC57-mitoLbNOX

    Plasmid

    #74448

    Purpose

    This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

    Depositor

    Vamsi Mootha

    Article

    Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

    Insert

    mitoLbNOX
    

    Use

    Tags

    Flag tag with linker and mitochondrial targeting …

    Expression

    Mutation

    Human codon optimized

    Promoter

    Available since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB_tre_dCas9_KRAB

    Plasmid

    #126030

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    dCas9 fusion with KRAB
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    EF1 alpha and TRE

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)Gα15-244-NLuc

    Plasmid

    #207154

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA15 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Dominique Schols, Tom Van Loy

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNA15 (GNA15 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position E(244)N

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB_tre_dCas9_VP160

    Plasmid

    #126031

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    dCas9 fusion with VP160
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    EF1 alpha and TRE

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pInducer10-mir-RUP-PheS

    Plasmid

    #44011

    Purpose

    Lentiviral shRNA vector (pGIPz based) for inducible knockdown

    Depositor

    Stephen Elledge

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Insert

    PheS Gly294
    

    Use

    Lentiviral

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE2

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pINDUCER11 (miR-RUG)

    Plasmid

    #44363

    Purpose

    Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interest

    Depositor

    Thomas Westbrook

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Insert

    PheS Gly294
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. p5e irg1

    Plasmid

    #188698

    Purpose

    Gateway promoter element for the zebrafish irg1 gene

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Insert

    irg1 (acod1 Zebrafish)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    irg1

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. CAGGS-EGFP-NLS-3XFLAG-V5

    Plasmid

    #196090

    Purpose

    Expresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    GFP
    

    Use

    Tags

    C-terminal 3xFLAG and C-terminal V5

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAGGs

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTETRIS-cargo

    Plasmid

    #126033

    Purpose

    Luciferase reporter construct for the study of cis-repressive activity in long noncoding RNAs. Also confers puromycin resistance.

    Depositor

    Mauro Calabrese

    Article

    Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do

    Type

    Empty backbone

    Use

    Luciferase; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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