We narrowed to 9,360 results for: CAG

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pCAG-T3-SpCAS9-NG-pA

Plasmid

#131465

Purpose

Expresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

Insert

Codon optimized SpCas9-NG

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-hCASeGFP-pA

Plasmid

#59766

Purpose

Expresses CAS9-eGFP fusion protein in mammalian cells.

Depositor

Wataru Fujii

Article

Fujii et al Nucleic Acids Res. 2013 Aug 30.

Insert

CAS9

Tags

Flag and eGFP

Expression

Mammalian

Promoter

CAG

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-hPPP3R1

Plasmid

#179136

Purpose

Expression vector of human protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1), CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1 Human)

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6015nes

Plasmid

#108657

Purpose

A S6 Kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

hyBRET-S6K-EV

Tags

XFP

Expression

Mammalian

Promoter

pCAGGS

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-mTHC

Plasmid

#112620

Purpose

expression plasmid with CAG promoter driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-mTFP1 (H2BC3 Anthozoa, Human)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Tags

mTeal (mTFP1)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Ssh1

Plasmid

#163602

Purpose

Expresses Protein phosphatase Slingshot homolog 1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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KA1158_pPBCAG-MerCreMer-IZ

Plasmid

#124184

Purpose

piggyBac-based expression of the tamoxifen-inducible Cre

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

MerCreMer-IRES-Zeo

Expression

Mammalian

Promoter

CAG

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA

Plasmid

#136652

Purpose

Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9
sgRNA for St1Cas9
APOBEC-1 (APOBEC1 Human)
UGI
HA tag

Use

CRISPR and Synthetic Biology

Tags

2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

Expression

Mammalian

Promoter

CAG and U6

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS::AtHAP2-V5

Plasmid

#132958

Purpose

Express AtHAP2 full length protein in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

Insert

AtHAP2 (GCS1, HAP2 Mustard Weed)

Tags

V5

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Trex2

Plasmid

#79246

Purpose

Expression vector for Trex2, which is a non-processive 3' exonuclease.

Depositor

Jeremy Stark

Article

Bennardo et al PLoS Genet. 2009 Oct;5(10):e1000683. doi

Insert

Trex2 (Trex2 Mouse)

Expression

Mammalian

Promoter

pCAGGS

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-mCherry2-G

Plasmid

#164162

Purpose

Expresses mCherry2 under the control of CAG promoter, basic vector, multiclonig site at C terminal

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mCherry2

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.iGABASnFR.F102G

Plasmid

#112167

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

AAV

Mutation

F102G

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KanR neo

Plasmid

#167869

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG.Myc-SARS2_3CL

Plasmid

#200120

Purpose

Expresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

SARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)

Tags

Myc

Expression

Mammalian

Promoter

CAG

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_KanR neo

Plasmid

#167861

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Gdf9

Plasmid

#163599

Purpose

Expresses GDF-9 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf9 (Gdf9 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Rad18

Plasmid

#119003

Purpose

Mammalian expression of human Rad18 and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Rad18

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pPN10-gCAG

Plasmid

#114384

Purpose

pPN10 with gRNA for spCRISPR-Cas9 targeted to CAG repeat, PAM: CAG

Depositor

Vincent Dion

Article

Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

Insert

gCAG

Use

CRISPR

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-st1CAS9-pA

Plasmid

#131542

Purpose

Expresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Fujii et al Biochem Biophys Res Commun. 2016 Jun 16.

Insert

Codon optimized st1Cas9

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-V5-NLGN2

Plasmid

#82538

Purpose

pCAG vector for expressing V5-NLGN2

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

NLGN2 (Nlgn2 Rat)

Tags

V5

Expression

Mammalian

Promoter

CAG

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only