We narrowed to 54,988 results for: Ran

1. pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP

   Plasmid

   #185686

   Purpose

   Low-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERmembrane-GST-HaloTag-KKMP
   

   Expression

   Mammalian

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX408 Francisella tularensis subsp. novicida Cas9

   Plasmid

   #68705

   Purpose

   Francisella tularensis subsp. novicida Cas9

   Depositor

   Feng Zhang

   Insert

   FtCas9
   

   Use

   CRISPR

   Tags

   HA-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. delta PE OCT4-GFP-2A-PURO transgenic reporter

   Plasmid

   #52380

   Purpose

   BAC recombineering generated construct, used as a reporter for OCT4 expression

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Inserts

   GFP-2A-puro
   OCT4 (POU5F1 Human)
   

   Expression

   Mammalian

   Mutation

   deleted -1620 - -1011 upstream from OCT4 ATG

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)

   Plasmid

   #51656

   Purpose

   hsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and mOrange

   Depositor

   Adam Cohen

   Insert

   Quasar2-mOrange-Chr64-GFP
   

   Use

   Lentiviral

   Tags

   GFP and mOrange

   Expression

   Mammalian

   Promoter

   human synapsin

   Available Since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFUGW ORANGE Tubb3-GFP KI _ mCherry-KASH

   Plasmid

   #131508

   Purpose

   Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Tubb3, Tubb3 Rat, Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hUBC

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. delta DE OCT4-GFP-2A-PURO transgenic reporter

   Plasmid

   #52381

   Purpose

   BAC recombineering generated construct, used as a reporter for OCT4 expression

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Inserts

   GFP-2A-puro
   OCT4 (POU5F1 Human)
   

   Expression

   Mammalian

   Mutation

   deleted -3194 - -1768 upstream from OCT4 ATG

   Promoter

   OCT4

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

   Plasmid

   #128755

   Purpose

   Expresses CYRANO gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   CYRANO sgRNA (OIP5-AS1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. mouse deltaPE Oct4-GFP transgenic reporter

   Plasmid

   #52382

   Purpose

   BAC recombineering generated construct, used as a reporter for mouse Oct4 expression

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Inserts

   GFP pA
   Oct4 (Pou5f1 Mouse)
   PGK-gb2-Neo
   

   Expression

   Mammalian

   Mutation

   deleted -300 - -1300bp fragment upstream from Oct…

   Promoter

   Oct4 and PGK+gb2

   Available Since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. mouse Oct4-GFP GOF18 transgenic reporter

   Plasmid

   #60527

   Purpose

   BAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements)

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Insert

   GFPpA
   

   Expression

   Mammalian

   Promoter

   Oct4

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL4.23-IL2RA CaRE4 scramble (plus strand)

    Plasmid

    #91852

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0347)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE4 scramble (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Sequence scrambled from ch10:6094588-6094934

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid

    #116955

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMOS010: CheRiff-eGFP channelrhodopsin (plasma membrane)

    Plasmid

    #163044

    Purpose

    CheRiff-eGFP channelrhodopsin (plasma membrane)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    CheRiff
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTransgenesis p3 sv40 minimal promoter:: Katushka RFP

    Plasmid

    #131731

    Purpose

    Transposon based transgenesis

    Depositor

    Rene Maehr

    Article

    Stanney W et al Dev Biol. 2020 Mar 15;459(2):161-180. do

    Insert

    SV40MinPromoter
    

    Use

    Xenopus expression

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

    Plasmid

    #185687

    Purpose

    Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-GST-2xHaloTag-KKMP
    

    Expression

    Mammalian

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRK5 FLAG membrane-bound lipin-1 (TMD)

    Plasmid

    #120277

    Purpose

    Expression of ER/Golgi-tethered lipin-1 in mammalian cells

    Depositor

    Sirio Dupont

    Article

    Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

    Insert

    Lipin-1 (Lpin1 Mouse)
    

    Tags

    FLAG and in-frame fusion with the TMD Golgi-local…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Nuc-Orange Nano-lantern(Ca2+)/pcDNA3

    Plasmid

    #71349

    Purpose

    Express Nuc-Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cel

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Orange Nano-lantern(Ca2+)_H2B
    

    Expression

    Mammalian

    Available Since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pGL4.23-IL2RA CaRE4 SNP (plus strand)

    Plasmid

    #91851

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0346)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE4 SNP (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    rs61839660 C>T

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHDR-CDKN2A-Ex2-CMV-H2B-mOrange2

    Plasmid

    #110735

    Purpose

    Recombination template for replacing CDKN2A Exon2 with Ef1alpha-H2B-mOrange2

    Depositor

    Robert Judson-Torres

    Article

    Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

    Insert

    H2B-mOrange2
    

    Use

    Other

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFS_0272_pET30_pCat-tetR-Term-ptetA-Rluc-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid

    #116956

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-H2A-LSSmOrange2-2A-ERK-KTR-LSS-SGFP2

    Plasmid

    #129629

    Purpose

    Visualization of ERK activity

    Depositor

    Dorus Gadella

    Insert

    PB-H2A-LSSmOrange2-2A-ERK-KTR-LSS-SGFP2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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