We narrowed to 1,516 results for: pet-28a

1. pET28a-hDcp2 E147Q, E148Q

   Plasmid

   #72215

   Purpose

   bacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

   Depositor

   Megerditch Kiledjian

   Article

   Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

   Insert

   Dcp2 (DCP2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   E147Q, E148Q

   Promoter

   T7

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pET28a-Hook3 aa 1-160-GCN4

   Plasmid

   #74608

   Purpose

   Expresses artificially dimerized Hook domain of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-160 (HOOK3 Human)
   

   Use

   Tags

   6x His, GCN4, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. WT N-His Ana GvpC in pET28a

   Plasmid

   #153294

   Purpose

   Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

   Insert

   Gas vesicle protein C (N-terminal his-tagged)
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Added a hexahistidine tag after the methionine at…

   Promoter

   T7

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pF714-pET28a-Hs-FL-METTL20-NHis wt

   Plasmid

   #85115

   Purpose

   expression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human full-length METTL20 (METTL20 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

5. pET28a(+) 6xHis-TEV-IFIT5

   Plasmid

   #53560

   Purpose

   bacterial expression of IFIT5

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT5 (IFIT5 Human)
   

   Use

   Tags

   6xHIS and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pET28a-Hisx6-Sirt6-H133Y

   Plasmid

   #20279

   Depositor

   Leonard Guarente

   Article

   Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

   Insert

   Sirt6 (Sirt6 Mouse)
   

   Use

   Tags

   Hisx6

   Expression

   Bacterial

   Mutation

   Histidine 133 to Tyrosine

   Promoter

   Available Since

   March 24, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pET28a-Gluc-EL222-his

   Plasmid

   #172097

   Purpose

   Expresses the fusion protein of Gluc and the light-activated transcription factor EL222 in bacteria

   Depositor

   Avi Schroeder

   Article

   Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:

   Insert

   Gaussia Luciferase - EL222
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Gluc mutations - M43L, M110L

   Promoter

   T7

   Available Since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-28a(+)-MVK mazei

   Plasmid

   #108947

   Purpose

   heterologous expression of MVK of M. mazei in E. coli

   Depositor

   Frank Schulz

   Article

   Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

   Insert

   Mevalonate kinase (MVK)
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-MBP-(CBX1)3-eReader

   Plasmid

   #135090

   Purpose

   Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

   Depositor

   Marcey Waters

   Article

   Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

   Insert

   CBX1 chromobox protein 1, engineered x3
   

   Use

   Tags

   6xhis-tag and MBP

   Expression

   Bacterial

   Mutation

   lysine 43 mutated to alanine; aspartic acid 59 mu…

   Promoter

   T7

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-TAF4(827-909)

    Plasmid

    #105620

    Purpose

    bacterially express TAF4 HFD

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TAF4-HFD(827-909) (Taf4 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28A-p53 (1-73) (P12A P13A)

    Plasmid

    #62066

    Purpose

    Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains TAD residues 1-73; P12A, P13A

    Promoter

    T7

    Available Since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pET-28a(+)-MVK jannaschii

    Plasmid

    #108948

    Purpose

    heterologous expression of MVK of M. jannaschii in E. coli

    Depositor

    Frank Schulz

    Article

    Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

    Insert

    Mevalonate kinase (MVK)
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-Hisx6-Sirt6-S56A

    Plasmid

    #20278

    Depositor

    Leonard Guarente

    Article

    Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

    Insert

    Sirt6 (Sirt6 Mouse)
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Serine 56 to Alanine

    Promoter

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pET28a-His6-UROD-N367A

    Plasmid

    #236740

    Purpose

    Protein overexpression of human uroporphyrinogen decarboxylase N367A

    Depositor

    Christina Woo

    Article

    Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

    Insert

    Uroporphyrinogen decarboxylase human N367A (UROD Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    N367A

    Promoter

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-His6-GFP-UROD

    Plasmid

    #236741

    Purpose

    Protein overexpression of eGFP-human uroporphyrinogen decarboxylase

    Depositor

    Christina Woo

    Article

    Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

    Insert

    eGFP-Uroporphyrinogen decarboxylase human (UROD Human, Synthetic)
    

    Use

    Tags

    His6 and eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-28a PDZD8-T527D

    Plasmid

    #227851

    Purpose

    To express in E. coli PDZD8-T527D

    Depositor

    Sheng-cai Lin

    Article

    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

    Insert

    PDZD8 (PDZD8 Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    T527D

    Promoter

    Available Since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-28a PDZD8-T527E

    Plasmid

    #227852

    Purpose

    To express in E. coli PDZD8-T527E

    Depositor

    Sheng-cai Lin

    Article

    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

    Insert

    PDZD8 (PDZD8 Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    T527E

    Promoter

    Available Since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-hfYFP-TEV-Bcx

    Plasmid

    #191195

    Purpose

    Fusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    hfYFP & Bcx fusion
    

    Use

    Tags

    6xHis and Bacillus circulans xylanase

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-hfYFP-TEV-mScarlet-i

    Plasmid

    #191196

    Purpose

    Fusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    hfYFP & mScarlet-I fusion
    

    Use

    Tags

    6xHis and mScarlet-I

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-hfYFP-TEV-SAV

    Plasmid

    #191197

    Purpose

    Fusion of hfYFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    hfYFP & Streptavidin fusion
    

    Use

    Tags

    6xHis and Streptavidin core domain, amino acids 1…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge