We narrowed to 9,362 results for: Coli

1. pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2)

   Plasmid

   #169161

   Purpose

   To express nsp10-nsp14 fusion protein in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   14His-SUMO-nsp10-GGSGGS-nsp14 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-GFAP-iGluSnFR4s-PDGFR-WPRE

   Plasmid

   #234445

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4s-PDGFR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-GFAP-iGluSnFR4f-PDGFR-WPRE

   Plasmid

   #234446

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f-PDGFR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCJ208

   Plasmid

   #162681

   Purpose

   Lidded variant of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) removing a seven-residue loop of PETase (Trp185:Phe191, PETase numbering) and replacing it with Gly251:Thr472 from MHETase; codon optimized for expression in E. coli K12, with C-terminal His tag.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) incorporating the MHETase lid
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Replacement of W185-F191 with G251-T472 from MHET…

   Promoter

   T7/lac

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-syn-flex-iGluSnFR4s-PDGFR-WPRE

   Plasmid

   #234439

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4s-PDGFR
   

   Use

   AAV and Cre/Lox

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCJ217

   Plasmid

   #162685

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   G489C, S530C; codon optimized for E. coli K12

   Promoter

   T7/lac

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pK27Sumo_His-SUMO-nsp14-nsp10 fusion (SARS-CoV-2)

   Plasmid

   #169160

   Purpose

   To express nsp14-nsp10 fusion protein in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   14His-SUMO-nsp14-GGSGGS-nsp10 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   14His-SUMO and Fusion protein of SARS-CoV-2 nsp14…

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCJ209

   Plasmid

   #162682

   Purpose

   Lidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Replacement of G251-T472 with W185-F191 from PETa…

   Promoter

   T7/lac

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-CAG-iGluSnFR4s-PDGFR-WPRE

   Plasmid

   #234449

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4s-PDGFR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. YjdC-R82A_pET-14b

    Plasmid

    #233276

    Purpose

    Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 82 was substituted with alanine (R82A)

    Depositor

    Mohan Babu, Andrew Emili

    Article

    Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

    Insert

    YjdC (yjdC )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Arginine was replaced with Alanine at position 82…

    Promoter

    T7 promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. YjdC-R64A_pET-14b

    Plasmid

    #233263

    Purpose

    Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 64 was substituted with alanine (R64A)

    Depositor

    Mohan Babu, Andrew Emili

    Article

    Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

    Insert

    YjdC (yjdC )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Arginine was replaced with Alanine at position 64…

    Promoter

    T7 promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCJ200

    Plasmid

    #162673

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C and a sequence Tyr75:Phe104 to introduce a 6th disulfide bond as in AoFaeB-2.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    S136C and a sequence Y75-F104 to introduce a 6th…

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCJ210

    Plasmid

    #162683

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    S136C, G489C, S530C, and a sequence Y75-F104 to i…

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCJ220

    Plasmid

    #162686

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224W and C529S mutations from PETase active site.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with lid deletion and C224W and C529S mutations
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deltaG251-T472, C224W, C529S; codon optimized for…

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCJ221

    Plasmid

    #162687

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224H and C529F mutations from PETase active site.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with lid deletion and C224H and C529F mutations
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deltaG251-T472, C224H, C529F; codon optimized for…

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEANHISECArdB

    Plasmid

    #96967

    Purpose

    Expression of E. coli ArdB protein wirh an N-terminal TEV protease cleavable His tag

    Depositor

    James Naismith, Huanting Liu

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    E. coli ArdB
    

    Use

    Tags

    N-ter TEV cleavable 6His

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PS6K-mcherry-iATPsnFR1.1

    Plasmid

    #227238

    Purpose

    Inducible ATP sensor for E. coli

    Depositor

    Fuzhong Zhang

    Article

    Mu et al Nat Commun. 2024 Jun 21;15(1):5299. doi:

    Insert

    iATPsnFR1.1
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAIP4_3KT9

    Plasmid

    #234192

    Purpose

    Heterologous protein expression of aprataxin in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3KT9 (APTX Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pUK21

    Plasmid

    #49788

    Purpose

    E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)

    Depositor

    Joachim Messing

    Article

    Vieira et al Gene. 1991 Apr;100:189-94.

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    lacZ

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKM596

    Plasmid

    #8837

    Depositor

    David Waugh

    Article

    Fox et al FEBS Lett. 2003 Feb 27. 537(1-3):53-7.

    Type

    Empty backbone

    Use

    Tags

    E. coli MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge