We narrowed to 7,358 results for: Ank
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Plasmid#112371PurposeCRISPR donor plasmid to tag human transcription factor ELF4 with GFPDepositorInsertELF4 homology arms flanking EGFP-IRES-Neo cassette (ELF4 Human)
UseCRISPRAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVEZF1-donor
Plasmid#112341PurposeCRISPR donor plasmid to tag human transcription factor VEZF1 with GFPDepositorInsertVEZF1 homology arms flanking EGFP-IRES-Neo cassette (VEZF1 Human)
UseCRISPRMutationrs999907002, hg38:17:57975308:A>T, hg38:17:579…Available SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNR1D2-donor
Plasmid#112314PurposeCRISPR donor plasmid to tag human transcription factor NR1D2 with GFPDepositorInsertNR1D2 homology arms flanking EGFP-IRES-Neo cassette (NR1D2 Human)
UseCRISPRMutationhg38:3:23977483:A>T, hg38:3:23977515:A>G, h…Available SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPS3911
Plasmid#113330Purposecontrol for Hermes transposition; TIR-flanked LEU2, transposase absentDepositorInsertbeta-isopropyl malate dehydrogenase
ExpressionYeastAvailable SinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNJB99
Plasmid#51521PurposeGateway entry vector with attL1 and attR5 sites flanking Zif268:FokI coding sequenceDepositorInsertZif268:FokI
UseGateway entry vectorTagsNLSAvailable SinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pimEJ5GFP
Plasmid#44026DepositorInsertEJ5GFP egfp-based chromosomal break reporter
ExpressionMammalianMutationpCAGGS promoter and eGFP separated by pgkPURO cas…PromoterpCAGGSAvailable SinceMay 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB2223
Plasmid#67544PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTVGFP
Plasmid#169445PurposeA vector with two different MCSs flanking an attP integration site and a floxable pax-GFP markerDepositorTypeEmpty backboneUseTargeting vectorAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHEN2-SG4
Plasmid#196071PurposeExpress wild type SG4 in E.coliDepositorInsertSG4
Tags3XFLAG and 6XHisExpressionBacterialPromoterT7Available SinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAM_Ec
Plasmid#102939PurposeTransposon mutagenesis and Tn-seqDepositorInsertmariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase
ExpressionBacterialAvailable SinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain
Plasmid#228663PurposeBacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6)DepositorInsertZika virus portion of NS2B protease coexpressed with NS3 protease
TagsHis-GST-TEVExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
WT FL USP7
Plasmid#227214PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialPromoterT7-promoterAvailable SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor_mU6
Plasmid#69350PurposesgRNA scaffold and mouse U6 promoterDepositorInsertmU6 promoter and sgRNA scaffold flanked by BbsI sites
UseCRISPRPromotermU6 promoterAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
EV-A71 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag
Plasmid#204816PurposeBacterial expresison of Enterovirus A71 3C proteaseDepositorInsertEV A71 3C protease
ExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCfB2224
Plasmid#67547PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XI-2 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease fusion with non cleavable linker (N-terminal Twin-Strep-tag)
Plasmid#228661PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Zika virus for biochemical assayDepositorInsertZika virus portion of NS2B protease fused to NS3
TagsHis-Twin Strep-TevExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
DENV2 NS2B-NS3 protease: Co-expression of DENV-2 NS2B and NS3
Plasmid#204808PurposeBacterial co-expression of Dengue2 NS2B and NS3 proteinsDepositorInsertDengue2 NS2B (POLY Dengue2)
ExpressionBacterialAvailable SinceDec. 18, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
EV-A71 isolate 4643-TW98 2A precursor protease with C110A and four aa at N-term to create a VP1-2A junction in active site
Plasmid#228633PurposeBacterial expression of the A71 2A protease with ITTL at the N-terminal and a C110A mutation used for crystallization in P 6 1 (PDB 9FGO)DepositorInsertEnterovirus Coxsackievirus A71 2A protease
TagsHis-SUMOExpressionBacterialMutationC110AAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL4.75-KRAS LCS6m
Plasmid#44571DepositorInsertKRAS 3'UTR (KRAS Human)
ExpressionMammalianMutationThe T allele at LCS6 (let-7 complementary site 6)…PromoterCMVAvailable SinceApril 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Tm2AG
Plasmid#26773DepositorInsertTdTomato-2A-H2BGFP-SV40pA
ExpressionMammalianMutationmutation in 2A peptide that renders it non-functi…Available SinceMarch 14, 2011AvailabilityAcademic Institutions and Nonprofits only