19,653 results

1. pBT277

   Plasmid

   #122608

   Purpose

   express evoCDA1-BE4max in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   evoCDA1-BE4max
   

   Expression

   Mammalian

   Mutation

   F23S A123V I195F

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSQT1601

   Plasmid

   #53369

   Purpose

   human codon optimized Csy4 and FokI-dCas9 expression plasmid

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   hCsy4-T2A-NLS-hFokI-dCas9-NLS
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. Active Wnt3A-V5

   Plasmid

   #43810

   Depositor

   Xi He

   Article

   MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

   Insert

   WNT3A (WNT3A Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCh-Climp63

   Plasmid

   #136293

   Purpose

   expression of Climp63

   Depositor

   Gia Voeltz

   Article

   Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

   Insert

   Cytoskeletal-linking protein 63 (Ckap4 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMAZ-SK

   Plasmid

   #73962

   Purpose

   self-killing gRNA plasmid pMAZ-SK

   Depositor

   Alex Nielsen

   Article

   Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

   Insert

   gRNA
   

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG PSAM4 GlyR IRES EGFP

   Plasmid

   #119739

   Purpose

   Chemogenetic inhibitor expression plasmid

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Insert

   PSAM4 GlyR IRES eGFP (CHRNA7 Synthetic, Human)
   

   Expression

   Mammalian

   Mutation

   L131G, Q139L, Y217F

   Promoter

   CAG

   Available Since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. repressilator (pZS1-lTlrLLtCL)

   Plasmid

   #26489

   Depositor

   Michael Elowitz

   Article

   Elowitz et al Nature. 2000 Jan 20. 403(6767):335-8.

   Insert

   LacI, tetR
   

   Expression

   Bacterial

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX TRE 3G BFP

   Plasmid

   #128070

   Purpose

   Lentiviral expression of Tet inducible TagBFP

   Depositor

   Oskar Laur

   Insert

   TagBFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   no

   Available Since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.2 YFP

   Plasmid

   #84910

   Purpose

   expression of YFP in mammalian cells

   Depositor

   Aaron Gitler

   Article

   Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

   Insert

   YFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-synPCB2.1

    Plasmid

    #139478

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    MTS-Fnr-Fd-P2A-MTS-PcyA-FLAG-P2A-MTS-HA-HO1
    

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTCF158 Human beta-2 microglobulin

    Plasmid

    #180457

    Purpose

    E. coli expression of class I MHC light chain

    Depositor

    John Altman

    Insert

    Human beta-2 microglobulin
    

    Expression

    Bacterial

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMpGE014

    Plasmid

    #108682

    Purpose

    All-in-one genome editing vector in M. polymorpha (chlorsulfuron resistant)

    Depositor

    Takayuki Kohchi, Shigeo Sugano

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCCL-c-MNDU3-X

    Plasmid

    #81071

    Purpose

    Empty lentiviral backbone with the MNDU3 promoter

    Depositor

    Donald Kohn

    Article

    Logan et al Hum Gene Ther. 2004 Oct;15(10):976-88.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    MNDU3

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCh-Rab7A

    Plasmid

    #61804

    Purpose

    Human Rab7A fluorescently tagged with mCh on the N-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

    Insert

    Rab7A (RAB7A Human)
    

    Tags

    mCh

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. C1-F-tractin-mCherry

    Plasmid

    #155218

    Purpose

    Monitor global F-actin distribution

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa 9-52 of rat ITPKA) fused to mCherry (Itpka Rat)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. FLAG.PKCepsilon

    Plasmid

    #10795

    Depositor

    Alex Toker

    Article

    Cenni et al Biochem J. 2002 May 1. 363(Pt 3):537-45.

    Insert

    PKC epsilon (PRKCE Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRRL.SIN.EF1A.CD19-FMC63.218.CAR-2G

    Plasmid

    #194457

    Purpose

    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

    Depositor

    Ana Sofia Coroadinha

    Article

    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

    Insert

    Anti-CD19 CAR
    

    Use

    Lentiviral

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPolI_SARS-CoV-2_NLuc

    Plasmid

    #183953

    Purpose

    Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter

    Depositor

    AJ te Velthuis

    Article

    Vial et al bioRxiv 2021.06.28.450211

    Insert

    SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)
    

    Expression

    Mammalian

    Promoter

    Mammalian Pol I

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFRT/FLAG/HA-DEST QKI

    Plasmid

    #19891

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    quaking homolog, KH domain RNA binding (QKI Human)
    

    Tags

    FLAG/HA

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHEE401E

    Plasmid

    #71287

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    CRISPR; Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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