We narrowed to 9,085 results for: mel

1. pCS2+8-asp-2-flag-tev-halo-his

   Plasmid

   #227153

   Purpose

   For T-REX experiments of asp-2.

   Depositor

   Yimon Aye

   Article

   Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

   Insert

   asp-2 (asp-2 Nematode)
   

   Tags

   Flag, HaloTag, and His6

   Expression

   Mammalian

   Promoter

   CMV and SP6

   Available Since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTwist Amp_d5-HT7R-7xsfGFP11-HA-HDR-donor

   Plasmid

   #221818

   Purpose

   Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT7R coding frame

   Depositor

   Quentin Gaudry

   Article

   Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

   Insert

   7xGFP11-HA donor with 5-HT7R homology arms
   

   Use

   CRISPR

   Expression

   Insect and Mammalian

   Available Since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTwist Amp_d5-HT7R-sfGFP11-HA-CRISPR-HDR-donor

   Plasmid

   #221819

   Purpose

   Donor plasmid for inserting GFP11-HA to the end of Drosophila 5-HT7R coding frame

   Depositor

   Quentin Gaudry

   Article

   Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

   Insert

   GFP11-HA donor with homology arms
   

   Use

   CRISPR

   Expression

   Insect and Mammalian

   Available Since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTwist Amp_d5-HT1AR-7xsfGFP11-HA-HDR-donor

   Plasmid

   #221832

   Purpose

   Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT1AR coding frame

   Depositor

   Quentin Gaudry

   Article

   Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

   Insert

   7xGFP11-HA donor with 5-HT1AR homology arms
   

   Use

   CRISPR

   Expression

   Insect and Mammalian

   Available Since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTwist Amp_d5-HT2BR-7xsfGFP11-HA-HDR-donor

   Plasmid

   #221833

   Purpose

   Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2BR coding frame

   Depositor

   Quentin Gaudry

   Article

   Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

   Insert

   7xGFP11-HA donor with 5-HT2BR homology arms
   

   Use

   CRISPR

   Expression

   Insect and Mammalian

   Available Since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTwist Amp_2xFlag-dSERT-HDR-donor-HDR

   Plasmid

   #221835

   Purpose

   Donor plasmid for inserting 2xFLAG at the beginning of Drosophila SERT coding frame

   Depositor

   Quentin Gaudry

   Article

   Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

   Insert

   2xFLAG with dSERT homology arms
   

   Use

   CRISPR

   Expression

   Insect and Mammalian

   Available Since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTwist Amp_d5-HT1BR-7xsfGFP11-HA-HDR-donor

   Plasmid

   #221837

   Purpose

   Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT1BR coding frame

   Depositor

   Quentin Gaudry

   Article

   Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

   Insert

   7xGFP11-HA donor with 5-HT1BR homology arms
   

   Use

   CRISPR

   Expression

   Insect and Mammalian

   Available Since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTwist Amp_d5-HT2AR(isoformD)-7xsfGFP11-HA-HDR-donor

   Plasmid

   #221838

   Purpose

   Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2AR isoform D coding frame

   Depositor

   Quentin Gaudry

   Article

   Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

   Insert

   7xGFP11-HA donor with 5-HT2AR (isoform D) homology arms
   

   Use

   CRISPR

   Expression

   Insect and Mammalian

   Available Since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTwist Amp_d5-HT2AR(isoformBFH)-7xsfGFP11-HA-HDR-donor

   Plasmid

   #221839

   Purpose

   Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2AR isoforms B, F and H coding frame

   Depositor

   Quentin Gaudry

   Article

   Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

   Insert

   7xGFP11-HA donor with 5-HT2AR (isoforms BFH) homology arms
   

   Use

   CRISPR

   Expression

   Insect and Mammalian

   Available Since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPOTv7-hyg-OsAID (p5110)

    Plasmid

    #239360

    Purpose

    pPOTv7 based plasmid template for addition of a Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using hygromycin

    Depositor

    Mark Carrington

    Article

    Alexandra et al bioRxiv 2025.06.17.660065

    Inserts

    Rice Auxin Inducible Degradation domain
    Auxin Inducible Degradation domain
    

    Use

    Bacterial

    Promoter

    none

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPOTv6-puro-3Ty:OsAID:3Ty (p5229)

    Plasmid

    #239361

    Purpose

    pPOTv6 based plasmid template for addition of Ty-epitope tagged Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using puromycin

    Depositor

    Mark Carrington

    Article

    Alexandra et al bioRxiv 2025.06.17.660065

    Insert

    Rice Auxin inducible degradation domain
    

    Use

    Bacterial

    Tags

    3 x Ty epitope tag

    Promoter

    none

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPOTv6-hyg-3Ty:OsAID:3Ty (p5230)

    Plasmid

    #239362

    Purpose

    pPOTv6 based plasmid template for addition of Ty-epitope tagged Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using puromycin

    Depositor

    Mark Carrington

    Article

    Alexandra et al bioRxiv 2025.06.17.660065

    Insert

    Rice Auxin inducible degradation domain
    

    Use

    Bacterial

    Tags

    3 x Ty epitope tag

    Promoter

    n/a

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMT-EGFP-V5

    Plasmid

    #240240

    Purpose

    Expression of EGFP-V5 under control of copper-inducible promoter.

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    EGFP-V5
    

    Expression

    Insect

    Promoter

    MT

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR_HA-spGFP11

    Plasmid

    #240224

    Purpose

    Gateway entry clone for cloning in insert sequences by gibson assembly to create a C-terminal HA-spGFP11 tag. No ATG start codon.

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Type

    Empty backbone

    Use

    Gateway shuttle vector

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. GPR89B_full_pGCS1

    Plasmid

    #238499

    Purpose

    IVT mRNA of human gene

    Depositor

    Megan Dennis

    Article

    Soto et al bioRxiv [Preprint]. 2024 Sep 26:2024.09.

    Insert

    GPR89B (GPR89B Human)
    

    Expression

    Mammalian

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOP823

    Plasmid

    #235718

    Purpose

    Protein expression of His6-SUMO-HsRAB5A (Q79L C19S,C63S) 1-212 in bacteria for maleimide conjugation

    Depositor

    Roger Williams

    Article

    Buckles et al Biophys J. 2020 Dec 1;119(11):2205-2218.

    Insert

    RAB5A (RAB5A Human)
    

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    Q79L, C19S, C63S, aa 1-212

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLJC6-NADK2(delta MTS)-3xFLAG

    Plasmid

    #233896

    Purpose

    lentiviral overexpression vector for NADK2 (delta MTS)

    Depositor

    Jason Cantor

    Article

    Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

    Insert

    NADK2 (NADK2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    delta MTS (see Depositor Comments)

    Promoter

    Ubc

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiCRISPR-v1-sgNADK2

    Plasmid

    #233897

    Purpose

    lentiviral vector expressing Cas9 and an sgRNA targeting NADK2

    Depositor

    Jason Cantor

    Article

    Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

    Insert

    sgRNA targeting NADK2 (NADK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYO1645

    Plasmid

    #235719

    Purpose

    Protein expression of GST-tagged PX domain (Cys at 2 and C85S) in bacteria

    Depositor

    Roger Williams

    Article

    Buckles et al Biophys J. 2020 Dec 1;119(11):2205-2218.

    Insert

    p40phox PX domain (NCF4 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Cys at 2, C85S, aa 2-149

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTub-split-intein-Gal4[1-20]-N-int

    Plasmid

    #199210

    Purpose

    For ubiquitous expression of split-intein Gal4-N-int component under control of the tubulin promoter

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    split-intein Gal4[1-20]-N-int
    

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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