We narrowed to 22,195 results for: his

1. pEBTet-SNAP-Centrin2

   Plasmid

   #136784

   Purpose

   Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-Centrin2 (CETN2 Synthetic, Human)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pT7-agrBD-IV

   Plasmid

   #53440

   Purpose

   Derivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IV

   Depositor

   Cynthia Collins

   Article

   Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

   Insert

   agrBD locus (with D29Y mutation in agrD)
   

   Use

   Synthetic Biology

   Tags

   V5 epitope, 6x HIS (not expressed on agrB or agrD…

   Expression

   Bacterial

   Mutation

   D to Y mutation of residue 29 of the agrD gene (c…

   Promoter

   T7-lac

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET15b-Fab1 (Francisella tularensis)

   Plasmid

   #61690

   Purpose

   Inducible expression of the FabI enzyme (enoyl reductase) from Francisella tularensis

   Depositor

   Michael Johnson

   Article

   Hevener et al J Med Chem. 2012 Jan 12;55(1):268-79. do

   Insert

   enoyl-[acyl-carrier-protein] reductase (NADH)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

4. DCT.VV.Orf92

   Plasmid

   #12780

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf92
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 6, 2006

   Availability

   Academic Institutions and Nonprofits only

5. DCT.VV.Orf221

   Plasmid

   #12909

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf221
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 29, 2006

   Availability

   Academic Institutions and Nonprofits only

6. DCT.VV.Orf222

   Plasmid

   #12910

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf222
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 29, 2006

   Availability

   Academic Institutions and Nonprofits only

7. DCT.VV.Orf264

   Plasmid

   #12952

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf264
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 29, 2006

   Availability

   Academic Institutions and Nonprofits only

8. DCT.VV.Orf194

   Plasmid

   #12882

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf194
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pHM130Env

   Plasmid

   #137665

   Purpose

   Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

   Depositor

   Anjali Joshi

   Article

   Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

   Insert

   HIV-1 Envelope Glycoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-p160(1-200)

    Plasmid

    #43

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    p160 myb binding protein (Mybbp1a Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

11. hMEF2A K403R

    Plasmid

    #118355

    Purpose

    This plasmid expresses human MEF2A K403R mutant. This construct cannot undergo sumoylation on K403.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hMEF2A (MEF2A Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K403R

    Promoter

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTorPE-GEX-GECO1

    Plasmid

    #32464

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    GEX-GECO1.0
    

    Use

    Lab constructed

    Tags

    6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

    Expression

    Bacterial

    Mutation

    GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…

    Promoter

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET19b-hMED9

    Plasmid

    #15365

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Paoletti et al Proc Natl Acad Sci U S A. 2006 Dec 12. 1

    Insert

    hMED9 (MED9 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA-p160(400-580)

    Plasmid

    #45

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    p160 myb binding protein (Mybbp1a Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    expresses only aa400-580

    Promoter

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA-p160(200-400)

    Plasmid

    #44

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    p160 myb binding protein (Mybbp1a Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Expresses only aa200-400

    Promoter

    Available Since

    July 21, 2005

    Availability

    Academic Institutions and Nonprofits only

16. BRCA1-(1316-1538)

    Plasmid

    #35079

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 1316-1538

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

17. BRCA1-(498-663)

    Plasmid

    #35070

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 498-663

    Promoter

    T7

    Available Since

    April 6, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pSHS273 - Bacterial expression plasmid for SpCas9∆REC3 variant

    Plasmid

    #101202

    Purpose

    Bacterial expression plasmid for SpCas9∆REC3 variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant M1–N497,GGS,V713–D1368
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    M1–N497, GGS, V713–D1368

    Promoter

    T7

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. DF-UAS-spa-GCaMP6f

    Plasmid

    #67568

    Purpose

    Zebrafish expression of a photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6f
    

    Use

    Zebrafish transgenesis (see :a spinal opsin contr…

    Tags

    HIS-tag

    Expression

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant

    Plasmid

    #101233

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 2 variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant G582A/V583A/E584A/D585A/N588A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    G582A, V583A, E584A, D585A and N588A

    Promoter

    T7

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA6-E2F4CA-Myc

    Plasmid

    #80428

    Purpose

    Mammalian expression of constitutively active chick E2F4

    Depositor

    José María Frade

    Article

    Morillo et al Mol Cell Biol. 2012 Jul;32(14):2722-37.

    Insert

    E2F transcription factor 4 (E2F4 Chicken)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    T261E, T263E

    Promoter

    CMV

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28-p53 (1-73) (72R)

    Plasmid

    #62082

    Purpose

    Expression of human p53 (1-73) (72R) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    HIs

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; 72R

    Promoter

    T7

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

23. BRCA1-(540-758)

    Plasmid

    #35071

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 540-758

    Promoter

    T7

    Available Since

    May 14, 2012

    Availability

    Academic Institutions and Nonprofits only

24. DCT.VV.Orf76

    Plasmid

    #12764

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf76
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

25. pHM033Env

    Plasmid

    #137672

    Purpose

    Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

    Depositor

    Anjali Joshi

    Article

    Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

    Insert

    HIV-1 Envelope Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJSC272 - Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant

    Plasmid

    #101236

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K918A/V922A/R925A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K918A, V922A, R925A and Q926A

    Promoter

    T7

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. DCT.VV.Orf98

    Plasmid

    #12786

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf98
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

28. pQTEV-CUTC

    Plasmid

    #34801

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    cutC copper transporter homolog (E. coli) (CUTC Human)
    

    Use

    Tags

    His and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. DCT.VV.Orf78

    Plasmid

    #12766

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf78
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

30. pAC-luc-CLK

    Plasmid

    #33132

    Depositor

    Michael Rosbash

    Article

    Kadener et al Genes Dev. 2009 Sep 15;23(18):2179-91. E

    Insert

    CLK 3' UTR (Clk Fly)
    

    Use

    Luciferase

    Tags

    Firefly Luciferase

    Expression

    Insect

    Mutation

    Promoter

    Actin 5C

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

31. pET-22HT-MSI1(7-103)

    Plasmid

    #60357

    Purpose

    Express the RRM1 of mouse MSI1 in bacterial cells for protein purification

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-103) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    contains amino acids 7-103 of Msi1

    Promoter

    T7

    Available Since

    Nov. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

32. 4E2H.210_LUMABS

    Plasmid

    #155209

    Purpose

    Expresses 4E2H.210_LUMABS sensor in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    4E2H.210_LUMABS
    

    Use

    Tags

    6x His Tag

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. BRCA1-(360-498)

    Plasmid

    #35068

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 498-360

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

34. ADAP1

    Plasmid

    #163905

    Purpose

    Full length mouse ADAP1 for purification from insect cells

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    ADAP1 (Adap1 Mouse)
    

    Use

    Tags

    Cleavable 6 x HIS tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pQTEV-EPSTI1

    Plasmid

    #34826

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    epithelial stromal interaction 1 (EPSTI1 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSBL_DK198

    Plasmid

    #156425

    Purpose

    Insect cell expression of 3a delta N(aa 42-275)-sfGFP, needs to be introduced into baculoviral bacmid

    Depositor

    Stephen Brohawn

    Article

    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

    Insert

    SARS-CoV-2 3a protein (aa 42-275)
    

    Use

    Tags

    sfGFP-His

    Expression

    Insect

    Mutation

    aa 42-275

    Promoter

    Polyhedrin

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. FN(9-10)-I27-CC

    Plasmid

    #85394

    Purpose

    MTFM mechanosensor for integrin. Fibronectin domains 9&10 fused to titan immunoglobulin domain (I27) with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labeling

    Depositor

    Khalid Salaita

    Article

    Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:

    Insert

    Fibronectin (FN) domains 9 & 10 for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. BRCA1-(758-1057)

    Plasmid

    #35085

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 758-1057

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

39. pEBTetBl-CLIP-Cep57

    Plasmid

    #136866

    Purpose

    Mammalian expression of the centrosomal protein Cep57 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Cep57 (CEP57 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. DCT.VV.Orf86

    Plasmid

    #12774

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf86
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 6, 2006

    Availability

    Academic Institutions and Nonprofits only