We narrowed to 8,675 results for: App

1. pMGF172

   Plasmid

   #96998

   Purpose

   ish1 mCherry insertion cassette with ura4+ gene

   Depositor

   Wesley Sundquist, Adam Frost

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   ish1 (ish1 Fission Yeast)
   

   Use

   Tags

   GST and mCherry

   Expression

   Bacterial

   Mutation

   ish1 C-terminal domain fused with mCherry + ura4(…

   Promoter

   N/A

   Available since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDONR-IAA1_no stop

   Plasmid

   #83847

   Purpose

   Gateway donor vector for IAA1 with no stop codon

   Depositor

   Jennifer Nemhauser

   Article

   Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

   Insert

   IAA1 (IAA1 Mustard Weed)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   no stop codon

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFRT-TODestFLAGHA_RBPMS_KDR

   Plasmid

   #65091

   Purpose

   Destination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) for stable cell line generation

   Depositor

   Thomas Tuschl

   Article

   Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   changed sequence to render plasmid resistant to s…

   Promoter

   CMV, 2x Tet

   Available since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-EngHD

   Plasmid

   #64033

   Purpose

   Mammalian expression of TTF-1 homeodomain fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)

   Depositor

   Daniel Christophe

   Article

   Christophe-Hobertus et al Mol Cell Endocrinol. 2007 Jan 29;264(1-2

   Insert

   TTF-1 (TTF1 dog)
   

   Use

   Tags

   Eng (N-terminal repressor domain of the drosophi…

   Expression

   Mammalian

   Mutation

   homeodomain, aa 154-226

   Promoter

   CMV

   Available since

   April 23, 2015

   Availability

   Academic Institutions and Nonprofits only

5. Cs2+Etv4 S100D

   Plasmid

   #49094

   Purpose

   expression of Etv4 S100D mutant

   Depositor

   Michael Tsang

   Article

   Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

   Insert

   Etv4 S100D (etv4 Zebrafish)
   

   Use

   Xenopus/avian/zebrafish

   Tags

   Expression

   Mammalian

   Mutation

   S100D

   Promoter

   CMV

   Available since

   Nov. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCR4-*mRFP

   Plasmid

   #47118

   Purpose

   Can be used to make an RNA probe for whole-mount in situ hybridization, for in-frame fusion proteins or as a subcloning step for mRFP. Note that the coding sequence for mRFP lacks the start codon.

   Depositor

   Stephen Ekker

   Article

   Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

   Insert

   *mRFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   No start codon

   Promoter

   Lac

   Available since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSG5-H-Ras_V12-A38

   Plasmid

   #39513

   Depositor

   Julian Downward

   Article

   Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

   Insert

   HRAS V12 A38 (HRAS Human)
   

   Use

   Eukaryotic

   Tags

   Expression

   Mutation

   G12V and D38A

   Promoter

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pSG5-H-Ras_V12-E38

   Plasmid

   #39514

   Depositor

   Julian Downward

   Article

   Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

   Insert

   HRAS V12 E38 (HRAS Human)
   

   Use

   Eukaryotic

   Tags

   Expression

   Mutation

   G12V and D38E

   Promoter

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pMC-cIAP1-FL

   Plasmid

   #20209

   Depositor

   Martin Holcik

   Article

   Zhao et al Cell Death Differ. 2009 Feb . 16(2):244-

   Insert

   cIAP1 3'UTR (BIRC2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-FLAG-RNMT 1-120

    Plasmid

    #112706

    Purpose

    Expresses N-terminally FLAG-tagged human RNMT 1-120 in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    RNMT (RNMT Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    deleted amino acids 121-476

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-FLAG-RNMT 121-476

    Plasmid

    #112707

    Purpose

    Expresses N-terminally FLAG-tagged human RNMT 121-476 in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    RNMT (RNMT Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    deleted amino acids 2-120

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDSM-de-Ppgk1-PYC-Ttpi1

    Plasmid

    #127735

    Purpose

    Yeast pathway position 5. PYC transcription unit with the PGK1 promoter and TPI1 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    pyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Ppgk1

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDSM-de-Ppxr1-PYC-Tnat1

    Plasmid

    #127736

    Purpose

    Yeast pathway position 5. PYC transcription unit with the PXR1 promoter and NAT1 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    pyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Ppxr1

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV CAMKII PSAM4 GlyR IRES EGFP

    Plasmid

    #119744

    Purpose

    Chemogenetic inhibitor expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Has Service

    AAV5

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    CamKII

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV SYN PSAM4 GlyR IRES EGFP

    Plasmid

    #119742

    Purpose

    Chemogenetic inhibitor expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Has Service

    AAV5

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    synapsin

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p3E-WPRE-SV40-pA (JDW 922)

    Plasmid

    #224545

    Purpose

    A Gateway compatible 3' entry clone containing a woodchuck herpes simplex virus regulatory element (WPRE) to stabilize mRNA upstream of an SV40 polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    WPRE-SV40-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EGFR L858R

    Plasmid

    #11012

    Purpose

    Retroviral construct for expressing human EGFR with L858R mutation in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR L858R (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    L858R (tumor-derived)

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TK-GFP-Nluc-P2A-neo-TK

    Plasmid

    #134896

    Purpose

    Expresses GFP protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum TK locus

    Depositor

    David Sibley

    Article

    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

    Insert

    TK-GFP-Nluc-P2A-Neo-TK
    

    Use

    Cryptosporidium expression

    Tags

    Expression

    Mutation

    Promoter

    Cryptosporidium actin and enolase

    Available since

    Nov. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CROP-seq-puro-v2-no-scaffold

    Plasmid

    #228521

    Purpose

    CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences

    Depositor

    Jellert Gaublomme

    Article

    Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

    Inserts

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CROP-seq-mTurquoise2-v2-no-scaffold

    Plasmid

    #228522

    Purpose

    CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using mTurquoise2 as selection marker

    Depositor

    Jellert Gaublomme

    Article

    Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

    Insert

    mTurquoise2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge