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pSico PGK GFP

Plasmid

#12093

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes the EGFP gene to be recombined out of the construct, activating shRNA expression.

Depositor

Tyler Jacks

Article

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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TEV Protease-mCherry

Plasmid

#243793

Purpose

Encodes for mammalian expression of TEV protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

TEV protease

Expression

Mammalian

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_35bpLinker

Plasmid

#157790

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 35 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_35bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pADH119

Plasmid

#90985

Purpose

NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb LEUpOUT CRISPR system.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Insert

gRNA conserved, C. albicans LEU2 2 of 2

Use

CRISPR

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO-mito-tagRFP

Plasmid

#185599

Purpose

Lentiviral construct for expression of tagRFP-T targeted to mitochondria

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

mito-tagRFP-T

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-SENP3-C526A

Plasmid

#115522

Purpose

Mammalian expression construct

Depositor

Martine Roussel

Article

Kuo et al Cell Cycle. 2008 Nov 1;7(21):3378-87. do

Insert

SENP3-C526A (Senp3 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

C526A

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaI

Plasmid

#115268

Purpose

Expresses EiCsm6 from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

EiCsm6

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCI-neo Fluc EGFP

Plasmid

#90170

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Expression

Mammalian

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_15bpLinker

Plasmid

#157786

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_15bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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H52-pUCM-hNGN2-inv

Plasmid

#110492

Purpose

Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm

Depositor

Michael Ward

Inserts

Neurogenin 2 (NEUROG2 Human)
mcherry
rtTA3G

Use

CRISPR, Cre/Lox, and TALEN

Expression

Mammalian

Promoter

CAG, EF-1 alpha, and TRE3G

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pBS MCK CRE

Plasmid

#12529

Depositor

Ronald Kahn

Article

Bruning et al Mol Cell. 1998 Nov . 2(5):559-69.

Insert

Cre recombinase

Use

Cre/Lox; Transgenic construct

Mutation

Cre is driven by the Muscle Creatine Kinase promo…

Available Since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_45bpLinker

Plasmid

#157791

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_45bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMF-TetTight

Plasmid

#193288

Purpose

Lentiviral construct that expresses rtTA-p2a-tTS for non-leaky dox-inducible expression of a transgene

Depositor

Rudolf Jaenisch

Article

Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

Insert

rtTA-P2A-tTS

Use

Lentiviral

Expression

Mammalian

Promoter

EF‐1α

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGEMT-PTE2A

Plasmid

#111773

Purpose

Quadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Type

Empty backbone

Use

Cloning intermediate

Promoter

T7

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-dCas9-VPR-pgk-hph

Plasmid

#233066

Purpose

Piggybac CRISPRa construct

Depositor

Markus Landthaler

Article

Vučićević et al bioRxiv 2024.09.12.612198

Insert

dCas9-VPR

Use

CRISPR

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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TRE-mCherry-p2A-bReaches-TS

Plasmid

#158701

Purpose

Reporter construct

Depositor

Alice Ting

Article

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

mCherry-p2A-bReaches-TS

Use

AAV

Tags

mCherry

Expression

Mammalian

Promoter

TRE

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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hPGK-rtTA3

Plasmid

#195883

Purpose

Lentiviral construct of hPGK driven rtTA3

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

rtTA3

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hPGK

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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Lyn-R-GECO1

Plasmid

#120410

Purpose

Expresses a fusion between myristoylation signal peptide and R-GECO1. This construct targets R-GECO1 to the plasma membrane.

Depositor

Won Do Heo

Article

Kim et al Proc Natl Acad Sci U S A. 2016 May 24;11

Insert

Lyn-R-GECO1

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pFUGW-hGtACR2-EYFP

Plasmid

#67877

Purpose

Mammalian expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone

Depositor

John Spudich

Article

Govorunova et al Science. 2015 Jun 25. pii: aaa7484.

Insert

hGtACR2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Promoter

UbC

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO-perox-tagRFP

Plasmid

#185600

Purpose

Lentiviral construct for expression of tagRFP-T targeted to peroxisomes

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

perox-tagRFP-T

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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