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We narrowed to 7,064 results for: poly

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Showing: 5461 - 5480 of 7064 results

  1. PiggyBac CAG eGFP-PolQ10A-FLAG P2A-BLAST

    Plasmid
    #249812
    Purpose
    Expresses the polymerase theta protein fused with N-terminal eGFP and C-terminal FLAG tag and mutated at serines 1289, 1482, 1486, 1488, 1493, 1555, 1563, 1628, 1635 and threonine 1755 into alanines
    Depositor
    Raphael Ceccaldi
    Article
    Gelot et al Nature. 2023 Sep;621(7978):415-422. doi:
    Insert
    Polymerase Theta (POLQ Human)
    Tags
    FLAG and eGFP
    Expression
    Mammalian
    Mutation
    serines 1289, 1482, 1486, 1488, 1493, 1555, 1563,…
    Promoter
    CAG
    Available Since
    March 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    249812-plasmid-map-sequence-id-502074

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  2. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid
    #61364
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61364-plasmid-map-sequence-id-99800

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  6. pcDNA3.1_ spike_del19

    Plasmid
    #155297
    Purpose
    Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles
    Depositor
    Raffaele De Francesco
    Article
    Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2
    Insert
    SARS-Cov2 spike_deleted (S SARS-Cov2)
    Use
    Generation of sars-cov-2-pseudotyped lentiviral p…
    Mutation
    Deletion in C-term 19 aa of spike
    Promoter
    CMV
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    155297-plasmid-map-sequence-id-307858

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  11. pLEW13

    Plasmid
    #24007
    Depositor
    George Cross
    Insert
    T7 RNA Polymerase
    Use
    Trypanosome expression
    Available Since
    May 11, 2010
    Availability
    Academic Institutions and Nonprofits only
    24007-plasmid-map-sequence-id-11278

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  12. pAcGFP-IVT

    Plasmid
    #205145
    Purpose
    IVT template of AcGFP with MCS in frame and polyA
    Depositor
    Igor Ulitsky
    Insert
    AcGFP1
    Use
    In-vitro transcription
    Promoter
    T7
    Available Since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    205145-plasmid-map-sequence-id-404881

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  13. pCS2FLAG

    Plasmid
    #16331
    Depositor
    Peter Klein
    Insert
    Flag tag
    Use
    Vertebrate expression
    Tags
    Flag
    Mutation
    Flag tag inserted into pCS2+ polylinker
    Available Since
    Dec. 21, 2007
    Availability
    Academic Institutions and Nonprofits only
    16331-plasmid-map-sequence-id-316908

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  14. pAcGFP-stop-IVT

    Plasmid
    #205146
    Purpose
    IVT template of AcGFP stop codon between the ORF and MCS and polyA
    Depositor
    Igor Ulitsky
    Insert
    AcGFP1
    Use
    In-vitro transcription
    Promoter
    T7
    Available Since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    205146-plasmid-map-sequence-id-404879

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  15. SV40-MCS-Z1

    Plasmid
    #127639
    Purpose
    Empty backbone plasmid with SV40 promoter and SV40 polyA with Zeocin/Bleocin bacterial resistance
    Depositor
    Jordan Green
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    SV40 promoter
    Available Since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    127639-plasmid-map-sequence-id-273360

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  17. pFFLuc-IVT

    Plasmid
    #205147
    Purpose
    IVT template of FireFly luciferase stop codon between the ORF and MCS and polyA
    Depositor
    Igor Ulitsky
    Insert
    FireFly luciferase
    Use
    Luciferase; In-vitro transcription
    Available Since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    205147-plasmid-map-sequence-id-495367

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  18. pRLuc-IVT

    Plasmid
    #205148
    Purpose
    IVT template of Renilla luciferase stop codon between the ORF and MCS and polyA
    Depositor
    Igor Ulitsky
    Insert
    Renilla Luciferase
    Use
    Luciferase; In-vitro transcription
    Promoter
    T7
    Available Since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    205148-plasmid-map-sequence-id-404883

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  19. pOpen-TthRT

    Plasmid
    #165566
    Purpose
    DNA polymerase that efficiently reverse-transcribes RNA in the presence of MnCl2
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Thermus thermophilus (Tth) RT
    Use
    Synthetic Biology
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165566-plasmid-map-sequence-id-340094

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  20. Hsp68-CreER(TM) (DM#264)

    Plasmid
    #15028
    Depositor
    Douglas Melton
    Insert
    Cre-ER(TM)
    Use
    Cre/Lox; For making transgenic constructs
    Tags
    Hsp68 promoter and polyA
    Expression
    Mammalian
    Available Since
    Oct. 18, 2007
    Availability
    Academic Institutions and Nonprofits only
    15028-plasmid-map-sequence-id-152167

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Showing: 5461 - 5480 of 7064 results