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pBW_0141

Plasmid

#102820

Purpose

pVec8 containing Sr45 gene

Depositor

Brande Wulff

Article

Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

Insert

wheat stem rust resistance gene Sr45 (NEWENTRY Synthetic)

Expression

Plant

Promoter

Sr33

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-A90V

Plasmid

#133754

Purpose

Lentiviral expression of human TDP-43 A90V disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TARDBP (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed alanine 90 to valine

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pUPD P35S (GB0552)

Plasmid

#160554

Purpose

35s promoter for N-terminal fusions

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Nucleic Acids Res. 2017 Feb 28;45(4):219

Insert

35s

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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P119 C-Check

Plasmid

#66817

Purpose

Dual-fluorescent surrogate reporter vector for in vitro functional assay of programable DNA nuclease and enrichment of genetically edited cells

Depositor

Yonglun Luo

Article

Zhou et al Cell Mol Life Sci. 2016 Jan 11.

Insert

truncated Enhanced Green Fluorescent Protein and asRED

Expression

Mammalian

Promoter

PGK and CMV

Available Since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-P112H

Plasmid

#133760

Purpose

Lentiviral expression of human TDP-43 P112H disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed proline 112 to histidine.

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-N-mEGFP-PARP11

Plasmid

#177998

Purpose

Expresses N-terminally mEGFP-tagged PARP11

Depositor

Karla Feijs

Article

Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

Insert

PARP11 (PARP11 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pICSL11055

Plasmid

#68252

Purpose

Level 1 Golden Gate Cassette: Plant kanamycin resistance cassette

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos

Use

Synthetic Biology

Expression

Plant

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-D169G

Plasmid

#133757

Purpose

Lentiviral expression of human TDP-43 D169G disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed aspartate 169 to glycine.

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsHelz_AG

Plasmid

#148799

Purpose

Mammalian Expression of HsHelz

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz (HELZ Human)

Expression

Mammalian

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe puro-HA-PIK3CA(H1047R;I848V)

Plasmid

#33253

Depositor

Thomas Roberts

Article

Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

Hemagglutinin (HA)

Expression

Mammalian

Mutation

changed histidine 1047 to arginine and isoleucine…

Promoter

SV40

Available Since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only
anxA10-GFP

Plasmid

#107199

Purpose

Expresses human annexin A10-GFP fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Quiskamp et al Cell Mol Life Sci. 2014 Jan;71(2):311-29

Insert

annexin A10 (ANXA10 Human)

Tags

Green Fluorescent Protein

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mCebpe

Plasmid

#79076

Purpose

Expression of mouse Cebpe

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Cebp epsilon (Cebpe Mouse)

Use

Lentiviral

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pLPC-Flag-CHES1L

Plasmid

#51836

Purpose

encodes the long isoform of human CHES1 and a Flag tag

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
SFG.CNa12_opt.IRES.eGFP

Plasmid

#22490

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

Insert

Codon optimised Calcineurin A mutant 12 (PPP3CA Human)

Use

Retroviral

Mutation

T351E; L354A

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCV-PIG-Lin28b

Plasmid

#64795

Purpose

Expresses human Lin28b in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Lin28b (LIN28B Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pPBpuro-ErbB1(DEL1011)

Plasmid

#197366

Purpose

Encoding human ErbB1 mutant.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

EGFR mutant. (EGFR Human)

Expression

Mammalian

Mutation

deleted amino acids 1011-1186

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe puro-HA-PIK3CA(H1047R;G320E)

Plasmid

#33254

Depositor

Thomas Roberts

Article

Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

Hemagglutinin (HA)

Expression

Mammalian

Mutation

changed histidine 1047 to arginine and glycine 32…

Promoter

SV40

Available Since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only
139H2_LC

Plasmid

#206202

Purpose

For recombinant expression of the light chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells.

Depositor

Joost Snijder

Article

Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202

Insert

anti-MUC1 antibody 139H2 light chain (Igkc Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC7A3

Plasmid

#132301

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Rebsamen et al Life Sci Alliance. 2022 Sep 16;5(11):e20

Insert

SLC7A3 (SLC7A3 Human)

Expression

Mammalian

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pMH-HT_CtCHAD-H253A-R256A-R260A-R296A-R418A-Y419A

Plasmid

#127790

Purpose

Expresses a CtCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain), mutations in His2…

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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