We narrowed to 10,047 results for: pET

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ZIKV_NASBA_27B

Plasmid

#75010

Purpose

Portion of Zika Virus genome (KU312312: 1248-1594) containing sensor 27B trigger sequence and NASBA amplification sites

Depositor

James Collins, Alexander Green

Publication

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_NASBA_27B

Expression

Bacterial

Promoter

T7

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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NEK1_HUMAN_D0

Plasmid

#79725

Purpose

This plasmid encodes the kinase domain of NEK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

NEK1 (NEK1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
STK3_HUMAN_D0

Plasmid

#79689

Purpose

This plasmid encodes the kinase domain of STK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

STK3 (STK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pCoofy17

Plasmid

#43991

Depositor

Sabine Suppmann

Publication

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His10-Sumo3

Expression

Bacterial

Promoter

T7

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pCoofy22

Plasmid

#43992

Depositor

Sabine Suppmann

Publication

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His10 and S

Expression

Bacterial

Promoter

T7

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pFX21

Plasmid

#44533

Depositor

Michael Grunstein

Publication

Xu et al Mol Cell. 2007 Sep 21;27(6):890-900.

Insert

SIR2-6xHis

Tags

His6

Expression

Bacterial

Available since

May 21, 2013

Availability

Academic Institutions and Nonprofits only
pBD-0082

Plasmid

#12369

Depositor

Axel Brunger

Publication

DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

Insert

VCP K524A (Vcp Mouse)

Tags

His6

Expression

Bacterial

Mutation

K524A

Available since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only
pBD-0050

Plasmid

#12362

Depositor

Axel Brunger

Publication

DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

Insert

VCP R586A (Vcp Mouse)

Tags

His6

Expression

Bacterial

Mutation

R586A

Available since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
HOXD4 (murine) MSCV

Plasmid

#8549

Depositor

Corey Largman

Publication

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXD4 (Hoxd4 Mouse)

Use

Retroviral

Tags

His and T7

Expression

Mammalian

Mutation

contains T7 and HIS tags from pET; makes protein …

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
CBS-6266

Plasmid

#253636

Purpose

dBa1Cas12a3 expression

Depositor

Chase Beisel

Publication

Dmytrenko et al Nature. 2026 Jan;649(8099):1312-1321. do

Insert

dBa1Cas12a2 E1032A C-terminal His tag

Tags

His

Expression

Bacterial

Mutation

E1032A

Promoter

T7

Available since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

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p300 KAT LIC 1M

Plasmid

#233587

Purpose

Bacterial expression of p300 KAT enzyme

Depositor

Scott Showalter

Publication

Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

Insert

p300 KAT (EP300 Human)

Tags

6xHis MBP

Expression

Bacterial

Promoter

T7

Available since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pEN017-SpCas9-hiNLS-t-M4

Plasmid

#234455

Purpose

Expresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGG

Depositor

Ross Wilson

Publication

Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

Insert

SpCas9 hiNLS t-M4

Use

CRISPR

Tags

His6, Nucleoplasmin, SV40, and c-Myc

Expression

Bacterial

Mutation

Cys at positions 80 and 574 have been mutated to …

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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RC(WW)-LgBit

Plasmid

#208931

Purpose

Recognition domain for in vitro MAPK-activity sensor

Depositor

Attila Reményi

Publication

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Insert

WW domain of PIN1 (PIN1 Human)

Tags

HISx6, LgBit, and MBP

Expression

Bacterial

Promoter

T7

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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p-G4PID

Plasmid

#209638

Purpose

Expression G4PID in mammalian cells

Depositor

Xiang Zhou

Publication

Lu et al Nucleic Acids Res. 2024 Mar 7:gkae126. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

G4PID (DHX36 Human)

Expression

Mammalian

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pRM053 His-TEV-Cas9 triNLS 0C

Plasmid

#196244

Purpose

Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

Depositor

Ross Wilson

Publication

Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

Insert

Cas9

Use

CRISPR

Tags

HA, His6 (N terminal on backbone), NLS, Nucleopla…

Expression

Bacterial

Mutation

Cys at positions 80 and 574 have been mutated to …

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pVSL10

Plasmid

#201182

Purpose

Bst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavable

Depositor

Jack Szostak

Publication

Lelyveld et al Science. 2023 Oct 27;382(6669):423-429.

Insert

Bst polymerase large fragment (BF) F710Y/D598A

Tags

HRV 3C protease cleavage site and His6

Expression

Bacterial

Mutation

F710Y D598A

Promoter

T5-lacO

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-88)-Strep

Plasmid

#196214

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-88)-Strep.

Depositor

Lalit Deshmukh

Publication

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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S100A6

Plasmid

#196217

Purpose

Codon-optimized bacterial expression plasmid. Expresses S100A6.

Depositor

Lalit Deshmukh

Publication

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

S100A6 (S100A6 Human)

Expression

Bacterial

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196, D40G)-Strep

Plasmid

#196210

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, D40G)-Strep.

Depositor

Lalit Deshmukh

Publication

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

D40G

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pHis-TEV-Nsp15

Plasmid

#187656

Purpose

E. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

Depositor

Debra Hansen

Publication

Jernigan et al Structure. 2022 Dec 30:S0969-2126(22)004

Insert

Nsp15 (N Severe acute respiratory syndrome coronavirus 2)

Tags

TEV protease cleavable His6-tag

Expression

Bacterial

Mutation

none

Promoter

T7lac

Available since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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