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pCEP4-BaLgp160-754*

Plasmid

#123212

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate; C-terminal truncation

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Premature stop co…

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLHCX-Flag-mPKM2(K433E)

Plasmid

#42514

Depositor

Dimitrios Anastasiou

Article

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

Pyruvate Kinase M2 Lys433->Glu

Use

Retroviral

Tags

Flag

Mutation

Changed lysine 433 to glutamate

Available Since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
pPotef-cdc3CB16-NatR

Plasmid

#86465

Purpose

Expresses an N-terminal Cdc3-mCherry fusion under the control of the constitutive Potef promotor. 16boxB stem-loops are integrated into the 3'UTR to visualize the mRNA.

Depositor

Michael Feldbrügge

Article

Baumann et al EMBO Rep. 2014 Jan;15(1):94-102. doi: 10

Insert

Cdc3

Use

Ustilago maydis expression

Tags

mCherry

Expression

Bacterial

Promoter

Potef

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-ING1b

Plasmid

#79052

Purpose

expression of p33ING1b in mammalian cells

Depositor

Karl Riabowol

Article

Scott et al J Cell Sci. 2001 Oct;114(Pt 19):3455-62.

Insert

ING1 (p33 ING1Ab isoform) (ING1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only
pEAK-bKal7

Plasmid

#145800

Purpose

expresses Kalirin-7 with the b-front in mammalian cells

Depositor

Betty Eipper, Richard Mains

Article

Miller et al J Neurochem. 2017 Mar;140(6):889-902. do

Insert

bKalirin-7 (Kalrn Rat)

Expression

Mammalian

Promoter

EIF2a

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-Arl13b2

Plasmid

#40871

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Gateway entry

Mutation

no stop codon

Available Since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only
pENTR223-BGLF5

Plasmid

#195813

Purpose

Gateway Entry Clone of Epstein-Barr virus BGLF5

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BGLF5 (BGLF5 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) neo with Ywhag CDS

Plasmid

#61799

Purpose

pcDNA3.1(+) with full length mouse Ywhag CDS

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

Ywhag CDS (Ywhag Mouse)

Expression

Mammalian

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-4SA(S109)

Plasmid

#33081

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S61A, S127A, S164A, S381A

Promoter

CMV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pMIG-Flag-RIPK1-wt

Plasmid

#102677

Purpose

retroviral expression plasmid for RIPK1-wt

Depositor

Hasam Habelhah

Article

Zhang et al Mol Cell Biol. 2015 Oct;35(19):3324-38.

Insert

RIPK1 (RIPK1 Human)

Use

Retroviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Promoter

unknown

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2-mCherry_SNAP_SNAPV_PACT_Dead

Plasmid

#173073

Purpose

Dead SNAP tag at the centrosome

Depositor

Heidi Hehnly

Article

Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

Insert

mCherry-SNAP-SNAP-Varied-PACT

Tags

mCherry-SNAP-SNAP-Varied

Expression

Mammalian

Mutation

AA144 in both SNAP tags changed from a cysteine t…

Promoter

CMV IE94

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-FKBP-mTagBFP2-SOScat

Plasmid

#178866

Purpose

Mammalian expression of SOS1(Rem-Cdc25) fused to FKBP-mTagBFP2

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

FKBP-mTagBFP2-SOS(Rem-Cdc25) (SOS1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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MAP2-CD4

Plasmid

#43916

Depositor

John Gearhart

Article

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Insert

CD4 (CD4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Human MAP2 promoter

Available Since

April 1, 2013

Availability

Academic Institutions and Nonprofits only
pSIREN-RetroQ-CDK4-shRNA

Plasmid

#25788

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

shRNA against human CDK4 (CDK4 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
pGL2-100bp

Plasmid

#16294

Depositor

Wafik El-Deiry

Article

Wang et al Cancer Res. 2006 Jul 15. 66(14):6982-9.

Insert

p53 promoter fragment (TP53 Human)

Use

Luciferase

Expression

Mammalian

Mutation

fragment -88 to +12 relative to first transcripti…

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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hSyn-GW-dCas9-SET2-IRES2-mCherry

Plasmid

#202089

Purpose

Neuron-specific expression vector of dCas9-Set2

Depositor

Elizabeth Heller

Article

Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

Insert

dCas9-Set2

Tags

3x FLAG and mCherry

Expression

Mammalian

Promoter

human Synapsin

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pKD280.7

Plasmid

#173172

Purpose

Expresses SO_4387

Depositor

Jeffrey Tabor

Article

Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

Insert

SO_4387

Expression

Bacterial

Promoter

J23107

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSIN-Skil-gRNA2

Plasmid

#180371

Purpose

targeting mouse Skil/SnoN gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Skil targeting gRNA (Skil Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

human U6

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pVPS13D-I-EGFP

Plasmid

#176464

Purpose

express internally GFP-tagged VPS13D in mammalian cells

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Tags

GFP-Ub

Expression

Mammalian

Mutation

EYFP is internally inserted

Promoter

CMV

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pET41a iGluh

Plasmid

#119830

Purpose

Production of recombinant iGluSnFR E25A high affinity variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluh

Tags

GST

Expression

Bacterial

Mutation

E25A

Promoter

T7

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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Talin Head

Plasmid

#186140

Purpose

For expression of recombinant Talin head domain in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

TLN1 (TLN1 Human)

Tags

His6 (tev cleavage)

Expression

Bacterial

Promoter

Lac

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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ELC-Fc

Plasmid

#8636

Depositor

Timothy Springer

Article

Manjunath et al J Clin Invest 2001 Sep;108(6):871-8.

Insert

CCL19 (CCL19 Human)

Expression

Mammalian

Available Since

June 23, 2005

Availability

Academic Institutions and Nonprofits only
pMH-SFB-RNF169

Plasmid

#99392

Purpose

Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged RNF169

Depositor

Michael Huen

Article

Chen et al J Biol Chem. 2012 Aug 10;287(33):27715-2

Insert

RNF169 (RNF169 Human)

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pETM33_PEX14_Pex14

Plasmid

#178484

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

PEX14_Pex14 (PEX14 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+MT-hToca-1-MGD

Plasmid

#33033

Depositor

Marc Kirschner

Article

Ho et al Cell. 2004 Jul 23;118(2):203-16.

Insert

Toca-1 (FNBP1L Human)

Tags

6xMyc

Expression

Mammalian

Mutation

MGD = amino acid substitutions M383I, G384S and D…

Promoter

simian CMV IE94

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-myc3-CUL4A delta N52

Plasmid

#19952

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CUL4A (cullin 4A) (CUL4A Human)

Tags

myc3

Expression

Mammalian

Mutation

Missing 52 residues from N terminal CUL4A.

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only
plko.1/shMCT4 477

Plasmid

#99598

Purpose

Knockdown of MCT4 expression

Depositor

Eli Bar

Article

Lim et al Oncogene. 2014 Aug 28;33(35):4433-41. do

Insert

MCT4 (SLC16A3 Human)

Use

Lentiviral

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pAcGP67A-rCL1-HormR-GAIN

Plasmid

#37847

Depositor

Axel Brunger

Article

Arac et al EMBO J. 2012 Feb 14;31(6):1364-78. doi:

Insert

CL1 (Adgrl1 Rat)

Use

Baculovirus transfer vectors

Tags

8xHIS

Mutation

HormR and GAIN domains only (aa460–849

Promoter

Polyhedrin

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3.1(-) + SLNCR1

Plasmid

#86816

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pME DN-E-cadherin

Plasmid

#135209

Purpose

Dominate Negative zebrafish e-cadherin label

Depositor

David Tobin

Article

Cronan et al Immunity. 2016 Oct 18;45(4):861-876. doi

Insert

CDH1

Use

Synthetic Biology

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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