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pDONR221-PARP7

Plasmid

#184474

Purpose

For gateway cloning of PARP7

Depositor

Karla Feijs

Article

Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

Insert

PARP7 (TIPARP Human)

Use

Gateway cloning

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-PARP15

Plasmid

#184471

Purpose

For gateway cloning of PARP15

Depositor

Karla Feijs

Article

Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

Insert

PARP15 (PARP15 Human)

Use

Gateway cloning

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA_SRR134.3_miRFP670

Plasmid

#163754

Purpose

gRNA expression vector containing the miRFP670 fluorescent marker to target the region downstream of the SRR134 SOX2 enhancer in human cells.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

gRNA_SRR134.3

Use

CRISPR

Tags

miRFP670

Expression

Mammalian

Promoter

U6

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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300_pETcon_SARS2_FLip

Plasmid

#222230

Purpose

yeast surface display of the SARS-CoV-2 FLip variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

Insert

SARS-CoV-2 FLip RBD (S SARS-CoV-2 virus, Budding Yeast)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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297_pETcon_SARS2_Omicron-BA286

Plasmid

#222231

Purpose

yeast surface display of the SARS-CoV-2 BA.2.86 variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

Insert

SARS-CoV-2 BA.2.86 RBD (S SARS-CoV-2 virus, Budding Yeast)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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299_pETcon_SARS2_EG-5

Plasmid

#222232

Purpose

yeast surface display of the SARS-CoV-2 EG.5 variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

Insert

SARS-CoV-2 EG.5 RBD (S SARS-CoV-2 virus, Budding Yeast)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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A99-ELP

Plasmid

#170760

Purpose

A99 laminin α1 peptide fused to I30-ELP (VPGIG)30

Depositor

Andrew MacKay

Article

Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

Insert

A99-ELP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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AG73-ELP

Plasmid

#170762

Purpose

AG73 laminin α1 peptide fused to I30-ELP (VPGIG)30

Depositor

Andrew MacKay

Article

Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

Insert

AG73-ELP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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MAC3_C_VPS35L(A830T)

Plasmid

#209693

Purpose

Expresses MAC3-tagged VPS35L(A830T) in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

VPS35L (VPS35L Human)

Tags

MAC3-tag

Expression

Mammalian

Mutation

A830T, changed Alanine 830 to Threonine

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAGK43

Plasmid

#222223

Purpose

Edits NPAS2 Gene.

Depositor

Seth Shipman

Article

Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

Eco1 RT; 112 nt donor; PAM -19 (NPAS2 Human)

Use

CRISPR

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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TCF4 - P381L in pENTR

Plasmid

#216570

Purpose

gateway cloning donor vector containing TCF4 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

TCF4 (TCF4 Human)

Use

Gateway entry plasmid

Mutation

P381L

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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TCF4 - D424N in pENTR

Plasmid

#216571

Purpose

gateway cloning donor vector containing TCF4 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

TCF4 (TCF4 Human)

Use

Gateway entry plasmid

Mutation

D424N

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-APP-CTF

Plasmid

#181732

Purpose

Galactose inducible expression of APP-CTF

Depositor

Alejandro Chavez

Insert

APP-CTF (APP Human)

Expression

Yeast

Mutation

C-terminal fragment (aa 671-770)

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb12622

Plasmid

#219929

Purpose

PrACT with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter ACT

Expression

Yeast

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.B-GS3

Plasmid

#204760

Purpose

Expression of Cas9 and human H2A.B

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AB1 (H2AFB1 Human)

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH3.2-GS3

Plasmid

#204763

Purpose

Expression of Cas9 and human H3.2

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H3.2 (HIST2H3PS2 Human)

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.L-GS3

Plasmid

#204756

Purpose

Expression of Cas9 and human H2A.L

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AL3 (H2AC6 Human)

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb12562

Plasmid

#219923

Purpose

PrGPD with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter GPD

Expression

Yeast

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13186

Plasmid

#219924

Purpose

PrTEF with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter TEF

Expression

Yeast

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb12561

Plasmid

#219922

Purpose

PrPGM with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter PGM

Expression

Yeast

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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