We narrowed to 66,707 results for: nin
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Plasmid#213510PurposeattB-containing pDEST plasmid, for use in Multisite Gateway reactionDepositorTypeEmpty backboneUseZebrafish expressionAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only
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pJMP1237
Plasmid#119262Purposefor cloning new sgRNAsDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pACEC3GH (Hyg)
Plasmid#110084PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneTags3C cleavage site - GFP - His10 TagExpressionBacterialAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIK86
Plasmid#47933PurposeC. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.DepositorPublicationInsertCas9
UseCRISPR and UnspecifiedPromoterT7Available sinceOct. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pAzF[TAG]
Plasmid#164579PurposeMachinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codonsDepositorInsertsMj pCNFRS[TAG] (aaRS1)
Mj pCNFRS[TAG] (aaRS2)
Mj tRNA[TAG]
ExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoteraraBAD, glnS, and proKAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A
Plasmid#154773Purposeplasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integrationDepositorInsertG1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
TagsFLAGExpressionMammalianMutationY125A in G1PylS, hybrid PylT with A41AA and C55A …PromoterEF1Available sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5/FRT/TO-GAlpha11-RLuc8
Plasmid#140983PurposeEncodes a G alpha subunit (GNA11) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAlpha11-RLuc8 (GNA11 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV 3xFLAG-LC3B-GFP
Plasmid#123107PurposeExpresses 3xFLAG-LC3B-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3BDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
Tags3xFLAG and EGFPExpressionMammalianPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-FLAG-MTOR-R2505P
Plasmid#69015Purposeactivating MTOR mutationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMTOR-R2505P (MTOR Human)
TagsFLAGExpressionMammalianMutationchange Arginine 2505 to ProlineAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-FLAG-MTOR-I2500F
Plasmid#69014Purposeactivating MTOR mutationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMTOR-I2500F (MTOR Human)
TagsFLAGExpressionMammalianMutationchange Isoleucine 2500 to PhenylalanineAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-OGT_opt
Plasmid#190858PurposeFor mammalian expression of wild-type full-length human ncOGT. The ncOGT is not epitope-tagged, but is codon-optimized for human cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertO-GlcNAc Transferase (OGT Human)
ExpressionMammalianMutationCodon optimized for expression in human cells.PromoterCMVAvailable sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET30-RT1306
Plasmid#131521PurposeExpresses engineered RT-1306 with His-tagDepositorInsertRT-1306
TagsHis-tagExpressionBacterialPromoterT7Available sinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pETconNK
Plasmid#81169PurposepETconNK Empty BackboneDepositorTypeEmpty backboneTagsc-mycExpressionBacterial and YeastPromoterGAL1Available sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGMCtN-TSC10M1-sspB
Plasmid#49521PurposeNourseothricin resistant; expresses sspB under the control of a promoter containing PtetO and constitutively expressses single-chain TetR10 (tsc10); integrates into mycobacteriophage att-Tweety siteDepositorPublicationInsertTSC10M1-sspB
Available sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAK_DR30_EF1a_CasRx_Puro
Plasmid#134842PurposeLentiviral all in one expression of CasRx and guideRNADepositorInsertRfxCas13d
UseCRISPR and LentiviralTagsT2A PuromycinExpressionMammalianPromoterEF1aAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAM5411
Plasmid#132663PurposeRSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistanceDepositorInsertsaadA
YFP
ExpressionBacterialPromoterPaadA and PconIIAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM5409
Plasmid#132662PurposeRSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistanceDepositorInsertsaadA
YFP
ExpressionBacterialPromoterPaadA and PconIIAvailable sinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-30-His6-SUMO-AEY-CfaN
Plasmid#122949PurposeBacterial expression of N-inteinDepositorInsertCfaN
ExpressionBacterialAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CellTagA
Plasmid#124591PurposeExpressed barcode for sample multiplexing in single-cell analysisDepositorInsertCellTagA
UseLentiviralExpressionMammalianPromoterhCMVAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SpyTag003-mClover3
Plasmid#133453PurposeExpresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasmDepositorInsertSpyTag003-mClover3
TagsGSGSGSGS linker and His6ExpressionBacterialPromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by