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pCMV-HA-MyD88

Plasmid

#12287

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

MyD88 (MYD88 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_H-Luc

Plasmid

#113924

Purpose

Plasmid encoding the bacterial expression of the chimeric HaloTag7-NanoLucCP fusion dubbed H-Luc, for BRET

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

H-Luc

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-TOM20_Halo_T2A_EGFP

Plasmid

#135443

Purpose

Mamalian cell expression of mictochondrial HaloTag7 and cytosolic EGFP

Depositor

Kai Johnsson

Article

Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

Insert

TOM20-HaloTag7 and EGFP

Tags

TOM20

Expression

Mammalian

Promoter

CMV/TetO

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pYS29

Plasmid

#202086

Purpose

Parent vector for producing universal minicircle donors for HaloTag N-terminal knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

NT HygR-T2A-Halo Minicircle Parent

Use

CRISPR

Tags

HaloTag

Promoter

None

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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mRuby2-N1

Plasmid

#54614

Purpose

Localization: N1 Cloning Vector, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Type

Empty backbone

Tags

mRuby2

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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mRuby2-C1

Plasmid

#54768

Purpose

Localization: C1 Cloning Vector, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Type

Empty backbone

Tags

mRuby2

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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mTFP1-N1

Plasmid

#54521

Purpose

Localization: N1 Cloning Vector, Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Type

Empty backbone

Tags

mTFP1

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pZF43x6_luciferase

Plasmid

#240267

Purpose

Circuit-responsive (ZF43RE promoter) expression of firefly luciferase. Used for evaluation of Nb139-VP64 transgene activity.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

firefly luciferase

Use

Luciferase

Expression

Mammalian

Promoter

ZF43x6-C

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb

Plasmid

#175544

Purpose

Expression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

IgKchL_HA-HaloTag9-myc_PDGFRtmb

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_CalR_HaloTag7_KDEL

Plasmid

#175530

Purpose

Expression of HaloTag7 in the endoplasmic reticulum of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

CalR-HaloTag7-KDEL

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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mOrange2-pBAD

Plasmid

#54531

Purpose

Localization: Protein Expression Vector , Excitation: 549, Emission: 565

Depositor

Michael Davidson, Nathan Shaner, Roger Tsien

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Tags

6xHis and mOrange2

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pYS17

Plasmid

#202083

Purpose

Parent vector for producing universal minicircle donors for mNeonGreen N-terminal knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

NT PuroR-T2A-mNG Minicircle Parent

Use

CRISPR

Tags

mNeonGreen

Promoter

None

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_CoA-Snifit(V97T)

Plasmid

#197944

Purpose

Expression of CoA-Snifit in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Expression

Mammalian

Mutation

PanK=PanK-V97T, D127A,L277W,F252Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_CoA-Snifit(G41)

Plasmid

#197942

Purpose

Expression of CoA-Snifit in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Expression

Mammalian

Mutation

PanK=PanK-D127A,L277W,F252Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_COX8-CoA-Snifit(G41S)

Plasmid

#197943

Purpose

Expression of CoA-Snifit in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Expression

Mammalian

Mutation

PanK=PanK-G41S, D127A,L277W,F252Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CoA-Snifit(V97T)

Plasmid

#197947

Purpose

Expression of CoA-Snifit in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Expression

Mammalian

Mutation

PanK=PanK-V97T, D127A,L277W,F252Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_HaloTag9_SKL

Plasmid

#175542

Purpose

Peroxysomal expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag9-SKL

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

Plasmid

#175534

Purpose

Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

IgKchL_HA-HaloTag7-myc_PDGFRtmb

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMEX174-hCALML3

Plasmid

#47600

Depositor

Emanuel Strehler

Article

Qian et al Protein Sci. 1998 Nov;7(11):2421-30.

Insert

CALM3 (CALML3 Human)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
mTagRFP-T-Endosomes-14

Plasmid

#58014

Purpose

Localization: Endosomes, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Endosomes (RHOB Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits