20,053 results

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pICH47742:: pRPS5a-PE2max-NC::tHSP

Plasmid

#227694

Purpose

Golden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.

Depositor

Jae-Yean Kim

Article

Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

Insert

PE2max-NC being driven by pRPS5a and terminated by tHSP

Use

CRISPR

Expression

Plant

Promoter

pRPS5a

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pH2B_mCherry_IRES_puro2

Plasmid

#21045

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

pH2B (H2BC16P Human)

Tags

mCherry

Expression

Bacterial and Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pCIN BRCA2 WT

Plasmid

#16245

Depositor

Mien-Chie Hung

Article

Wang et al Cancer Res. 2002 Mar 1. 62(5):1311-4.

Insert

BRCA2 (BRCA2 Human)

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pCS2 Flag-JunB

Plasmid

#29687

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

jun B oncogene (JUNB Human)

Tags

Flag

Expression

Mammalian

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mEGFP-TSG101

Plasmid

#116925

Purpose

expresses mEGFP-TSG101 in mammalian cells

Depositor

Sanford Simon

Article

Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

Insert

TSG101 (TSG101 Human)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pSBFP2-C1

Plasmid

#22880

Depositor

Dorus Gadella

Article

Kremers et al Biochemistry. 2007 Mar 27. 46(12):3775-8

Insert

SBFP2

Expression

Mammalian

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only

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Artichoke

Plasmid

#73320

Purpose

Artichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

SFFV and EF1a

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti6.2_TagBFP

Plasmid

#113724

Purpose

Expresses the fluorescent protein TagBFP in a lentiviral backbone

Depositor

Vanessa LaPointe

Insert

TagBFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-DHFR

Plasmid

#80329

Purpose

Allows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

DHFR-TSc3

Use

CRISPR

Promoter

DHFR-TS 5' UTR

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Klf4

Plasmid

#20322

Depositor

Rudolf Jaenisch

Article

Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

Insert

Klf4 (Klf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pHR_SFFv_Her2-BFP_RHL027

Plasmid

#164823

Purpose

Lentiviral vector for constitutive expression of HER2+TM(23-675)-BFP

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Insert

Extracellular and transmembrane region of ERBB2 (ERBB2 Human)

Use

Lentiviral

Tags

tagBFP

Promoter

SFFV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag FKHR

Plasmid

#13507

Depositor

Kunliang Guan

Article

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Insert

FKHR (FOXO1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HshnRNPK

Plasmid

#64923

Purpose

Expresses V5- and SBP-tagged hnRNPK in mammalian cells

Depositor

Elisa Izaurralde

Insert

hnRNPK (HNRNPK Human)

Tags

V5 and SBP

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCytERM_mScarlet-i_N1

Plasmid

#85068

Purpose

In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

CytERM

Tags

mScarlet-i

Expression

Mammalian

Mutation

aa1-29

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Peredox

Plasmid

#73807

Purpose

Expresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in mammalian cells (and can be packaged as AAV)

Depositor

Gary Yellen

Article

Mongeon et al Antioxid Redox Signal. 2016 Mar 18.

Insert

Peredox

Use

AAV

Expression

Mammalian

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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TLCV2-APOBEC1-YTHmut

Plasmid

#178950

Purpose

Lentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cells

Depositor

Kate Meyer

Article

Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

Insert

APOBEC1-YTHmut-T2A-GFP

Use

Lentiviral and Synthetic Biology; Inducible

Tags

HA

Expression

Mammalian

Promoter

tight TRE

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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DRD2-Tango

Plasmid

#66269

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

DRD2 (DRD2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR-53BP1 1-1972 WT

Plasmid

#53446

Purpose

Recombinational donor/master vector

Depositor

Daniel Durocher

Article

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Use

Gateway

Mutation

siRNA resistant and mutations A128V, T720A, A1347…

Available Since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA

Plasmid

#154867

Purpose

Flp-dependent hM4D(Gi) DREADD-mCherry

Depositor

Ulrik Gether

Article

Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03

Has Service

AAV Retrograde and AAV8

Insert

hM4D(Gi)-mCherry

Use

AAV; Flp-frt

Tags

mCherry

Expression

Mammalian

Promoter

Human Synapsin-1

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Flag-WTAP

Plasmid

#53741

Purpose

For expression of WTAP (Wilms tumor 1 associated protein) in human cells

Depositor

Chuan He

Article

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Wilms tumor 1 associated protein (WTAP Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

T7

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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