We narrowed to 5,564 results for: chia

1. pLXI_TRC401 LacZ

   Plasmid

   #111183

   Purpose

   Control inducible vector with LacZ (V5 tagged)

   Depositor

   William Hahn

   Article

   Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

   Insert

   lacZ
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   TRE

   Available since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAav-TP53-T2A-BirA*

   Plasmid

   #115652

   Purpose

   rAAV-based donor template for genome engineering of the TP53 protein C-terminus containing a T2A-BirA* module and a selection cassette

   Depositor

   Sven Eyckerman

   Article

   Vandemoortele et al J Proteome Res. 2018 Dec 10. doi: 10.102

   Inserts

   TP53 (TP53 Human)
   TP53 (TP53 Human)
   T2A
   BirA*
   

   Use

   AAV

   Tags

   BirA* and T2A

   Expression

   Mutation

   Homology region 1 and Homology region 2

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXPR_BRD003 Luciferase

   Plasmid

   #117072

   Purpose

   single guide RNA targeting Luciferase

   Depositor

   William Hahn

   Article

   Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

   Insert

   Luciferase
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLY54

   Plasmid

   #130923

   Purpose

   A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Inserts

   dcas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   

   Use

   Synthetic Biology

   Tags

   ASV tag and pspFΔHTH::λN22plus

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Anderson promoter: J23106, PpspA-LEA2B2, and Ptet

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. N229A (EcQ) MetNIQ in pBAD

   Plasmid

   #118257

   Purpose

   N229A Mutation in Methionine Binding Protein (MetQ) and Wild Type Methionine ABC Transporter for Transport Activity Assay

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Inserts

   MetN (metN E. coli)
   MetI (metI E. coli)
   MetQ (N229A) (metQ E. coli)
   

   Use

   Tags

   10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

   Expression

   Bacterial

   Mutation

   Changed Asparagine (Asn) 229 to Alanine (Ala) and…

   Promoter

   None and araBAD Promoter

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. N295A (EcN), N229A (EcQ) MetNIQ in pBAD

   Plasmid

   #118581

   Purpose

   N295A Mutation of MetN in Methionine ABC Transporter and N229A Mutation in Methionine Binding Protein (MetQ) for Transport Activity Assay

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Inserts

   MetN (metN E. coli)
   MetI (metI E. coli)
   MetQ (metQ E. coli)
   

   Use

   Tags

   10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

   Expression

   Bacterial

   Mutation

   Changed Asparagine (Asn) 229 to Alanine (Ala) and…

   Promoter

   None and araBAD Promotoer

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. N295A (EcN) MetNIQ in pBAD

   Plasmid

   #118256

   Purpose

   N295A Mutation of MetN in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Inserts

   MetN (N295A) (metN E. coli)
   MetI (metI E. coli)
   MetQ (metQ E. coli)
   

   Use

   Tags

   10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

   Expression

   Bacterial

   Mutation

   Changed Asparagine (Asn) 295 to Alanine (Ala)

   Promoter

   None and araBAD Promotoer

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. N295A (EcN), Y160A (EcI) MetNIQ in pBAD

   Plasmid

   #118260

   Purpose

   N295A Mutation of MetN and Y160A in MetI in Methionine ABC Transporter for Transport Activity Assay

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Inserts

   MetN (N295A) (metN E. coli)
   MetI (Y160A) (metI E. coli)
   MetQ (metQ E. coli)
   

   Use

   Tags

   10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

   Expression

   Bacterial

   Mutation

   Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …

   Promoter

   None and araBAD Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-iGluSnFR4s-PDGFR-WPRE

   Plasmid

   #234449

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4s-PDGFR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3xFLAG-DGKδ2-ΔSAMD

    Plasmid

    #226507

    Purpose

    Express N-terminal three tandem FLAG epitope tags, followed by an enterokinase cleavage site and human DGKD gene deleted SAM domain

    Depositor

    Chiaki Murakami, Fumio Sakane

    Article

    Murakami et al J Biol Chem. 2020 Mar 6;295(10):2932-294

    Insert

    DGKD (DGKD Human)
    

    Use

    Tags

    Three tandem FLAG epitope tag and enterokinase cl…

    Expression

    Mammalian

    Mutation

    deleted sterile alpha motif domain (deleted amino…

    Promoter

    CMV

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAcBac3-OMeYRS-8xYtR-EGFP-3TAA-40TGA-152TAG-TEV-3xUAA

    Plasmid

    #228787

    Purpose

    EcTyr aaRS/tRNA system for TAG suppression with EGFP containing three stop codons for triple suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcTyrRS
    EcYtR
    EGFP***
    TEV protease
    

    Use

    Baculoviral

    Tags

    Expression

    Mammalian

    Mutation

    TAG suppressor

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPICZ A-SLC[∆2-83]-His+MFN2-Strep (SB259)

    Plasmid

    #227608

    Purpose

    Inducible coexpression of His-tagged SLC25A46 in its N-terminal region (∆2-83) and Strep-tagged MFN2 in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Inserts

    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    

    Use

    Tags

    10xHis and Twin-StrepTag

    Expression

    Yeast

    Mutation

    aa 2-83 deleted

    Promoter

    AOX1

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPICZ A-SLC-His+MFN2-Strep (SB255)

    Plasmid

    #227604

    Purpose

    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Inserts

    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    

    Use

    Tags

    10xHis and Twin-StrepTag

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Anti-MBP [N128A/4R]

    Plasmid

    #206531

    Purpose

    Mammalian Expression Plasmid of anti-Maltose binding protein (MBP) (E. coli). Derived from hybridoma N128A/4.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Maltose binding protein (MBP) (Escherichia coli) recombinant Mouse monoclonal antibody (malE Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Anti-Thioredoxin [N245/44R]

    Plasmid

    #206546

    Purpose

    Mammalian Expression Plasmid of anti-Thioredoxin (E. coli). Derived from hybridoma N245/44.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Thioredoxin (Escherichia coli) recombinant Mouse monoclonal antibody (trxA Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNB1-LOX

    Plasmid

    #154058

    Purpose

    Level 1, Position 3 Golden Gate vector. ZmUbi-5'UTR:loxP-GUS-nosT-loxP-NAM-B1-nosT

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    LoxP-flanked GUS and TtNAM-B1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    The TtNAM-B1 gene sequence was domesticated to re…

    Promoter

    ZmUbi

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLY9

    Plasmid

    #130907

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-2G6, and Ptet

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLY59

    Plasmid

    #130927

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::MCP controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    sfgfp
    pspFΔHTH::MCP
    

    Use

    Synthetic Biology

    Tags

    ASV tag and MCP (MS2 coat protein)

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-2G6, and Ptet

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLY57

    Plasmid

    #130926

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA5B5 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA5B5, and Ptet

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLY56

    Plasmid

    #130925

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA4B4 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA4B4, and Ptet

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge