162,698 results

1. T7opt in pCAGGS

   Plasmid

   #65974

   Purpose

   Human codon-optimized T7 polymerase

   Depositor

   Benhur Lee

   Article

   Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

   Insert

   T7-opt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-HA-NONO

   Plasmid

   #127655

   Purpose

   Over-express HA-tagged human NONO

   Depositor

   Nicolas Manel

   Article

   Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

   Insert

   NONO (NONO Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLIB-HIS-TEV-CKS1

   Plasmid

   #177013

   Purpose

   Generate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEV

   Depositor

   Andrea Musacchio

   Article

   Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

   Insert

   CKS1 (CKS1B Human, Synthetic)
   

   Use

   Tags

   Polyhistidine-TEV

   Expression

   Insect

   Mutation

   codon-optimised for insect cell expression

   Promoter

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSAM_Cam

   Plasmid

   #91570

   Purpose

   Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.

   Depositor

   Harry Mobley

   Article

   Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pTRIPZ shSTING-Hsa

   Plasmid

   #128158

   Purpose

   Doxycyclin inducible shRNA knockdown of human STING gene

   Depositor

   Sandra Demaria

   Article

   Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

   Insert

   shSTING Hsa (STING1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNCS-Antares

   Plasmid

   #74279

   Purpose

   Constitutive expression of Antares in bacteria

   Depositor

   Michael Lin

   Article

   Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   Antares
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-LIC

   Plasmid

   #26094

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Tags

   6xHis and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   Aug. 20, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pMK289 (mAID-mClover-NeoR)

   Plasmid

   #72827

   Purpose

   C-terminal tagging with mAID-mClover using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-mClover-Neo
   

   Use

   CRISPR

   Tags

   mAID-mClover

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmal_c5x_RNAse_Inhib

   Plasmid

   #153314

   Purpose

   Bacterial expression of an RNase inhibitor

   Depositor

   Philippa Marrack, Drew Endy

   Insert

   RNAse Inhibitor
   

   Use

   Tags

   Maltose Binding Protein

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pX330 p53

    Plasmid

    #59910

    Purpose

    pX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoter

    Depositor

    Tyler Jacks

    Article

    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

    Insert

    sgRNA targeting p53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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