We narrowed to 9,384 results for: Coli

1. pUCXKT

   Plasmid

   #60682

   Purpose

   positive selection cloning and expression vector

   Depositor

   David Ackerley

   Article

   Prosser et al Biotechnol Lett. 2014 Sep 26.

   Type

   Empty backbone

   Use

   Positive selection e. coli expression vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-Strep-TEV-Nsp14

   Plasmid

   #154955

   Purpose

   Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli

   Depositor

   Justin Kollman

   Insert

   Strep-TEV-Nsp14 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Strep-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pET His10-Src42a

   Plasmid

   #126674

   Purpose

   Expression of His10-Src42a for purification from E. coli

   Depositor

   Ron Vale

   Article

   Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

   Insert

   Src42a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. enz.079-GCN5

   Plasmid

   #154046

   Purpose

   Expresses GCN5 in E. coli

   Depositor

   Christopher Voigt

   Article

   Wallace et al Advanced Functional Materials 2020

   Insert

   Histone acetyltransferase GCN5 (GCN5 Budding Yeast)
   

   Use

   Tags

   His, MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRD323

   Plasmid

   #198044

   Purpose

   Expression of HMfA K31A E35A in E. coli

   Depositor

   Remus Dame

   Article

   Erkelens et al Front Microbiol. 2023 Apr 18;14:1166608.

   Insert

   HMfA K31A E35A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-28a(+)-MVK mazei

   Plasmid

   #108947

   Purpose

   heterologous expression of MVK of M. mazei in E. coli

   Depositor

   Frank Schulz

   Article

   Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

   Insert

   Mevalonate kinase (MVK)
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCYP119

   Plasmid

   #50771

   Purpose

   expresses CYP 119 in e. coli

   Depositor

   Stephen Sligar

   Article

   McLean et al Biochem Biophys Res Commun. 1998 Nov 9;2

   Insert

   CYP119
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   April 28, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pET-28a(+)-HMGS

   Plasmid

   #108945

   Purpose

   heterologous expression of HMGS of E. faecalis in E. coli

   Depositor

   Frank Schulz

   Article

   Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

   Insert

   HMG-CoA synthase (HMGS)
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   A110G

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. GGDestX1-Amp

   Plasmid

   #157652

   Purpose

   Golden Gate assembly and cell-free (bacterial) expression of fusion-proteins

   Depositor

   Karmella Haynes

   Article

   Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

   Type

   Empty backbone

   Use

   Cell-free expression (e. coli sigma-70)

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   OR2-OR1-Pr

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSETA_ionKE

    Plasmid

    #172925

    Purpose

    Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

    Depositor

    Arnold Boersma

    Article

    Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

    Insert

    IonicStrengthSensorKE
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMAL-c5X-Dros16

    Plasmid

    #67004

    Purpose

    Expresses malE-Dros16 in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-Dros16
    

    Use

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-28a(+)-MVK jannaschii

    Plasmid

    #108948

    Purpose

    heterologous expression of MVK of M. jannaschii in E. coli

    Depositor

    Frank Schulz

    Article

    Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

    Insert

    Mevalonate kinase (MVK)
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28-Nsp14-TEV-Strep

    Plasmid

    #154953

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Nsp14-TEV-Strep (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    TEV-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pET22-Strep-TEV-Nsp10

    Plasmid

    #154958

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Strep-TEV-Nsp10 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Strep-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    July 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pET-28a(+)-IDI2

    Plasmid

    #108951

    Purpose

    heterologous expression of IDI2 of T. thermophilus in E. coli

    Depositor

    Frank Schulz

    Article

    Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

    Insert

    Isopentenyldiphosphat isomerase 2 (IDI2)
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCVD443

    Plasmid

    #66150

    Purpose

    cloning of EPEC E.coli eae gene

    Depositor

    James Kaper

    Article

    McDaniel et al Proc Natl Acad Sci U S A. 1995 Feb 28;92

    Insert

    eae
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pGEX-6P-1-GST-ATL(Cbx7)

    Plasmid

    #82526

    Purpose

    Expresses GST-CD (Cbx7) fusion proteins in E. coli. Cbx7 ATL region only, amino acids 66–81.

    Depositor

    Xiaojun Ren

    Insert

    Chromobox Homolog 7 (CBX7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    ATL(Cbx7), amino acids 66–81

    Promoter

    Available since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-28a(+)-AACT

    Plasmid

    #108944

    Purpose

    heterologous expression of AACT of M. sedula in E. coli

    Depositor

    Frank Schulz

    Article

    Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

    Insert

    Acetoacetyl-CoA synthase (AACT)
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMOD4 RT-G

    Plasmid

    #19184

    Depositor

    Al Fisher

    Article

    Zhang et al BMC Dev Biol. 2008 Dec 30;8(1):119.

    Insert

    rpsL tetA
    

    Use

    Recombineering

    Tags

    50 nucleotides of FLAG-GFP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMAL-c5X-CLP3.7

    Plasmid

    #67011

    Purpose

    Expresses malE-CLP3.7 in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-CLP3.7
    

    Use

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge